{"title":"Suitable dietary phospholipids improve lipid deposition, but excessive dietary phospholipids disorder lipid metabolism in juvenile leopard coral grouper (Plectropomus leopardus)","authors":"","doi":"10.1016/j.aqrep.2024.102265","DOIUrl":null,"url":null,"abstract":"<div><p>Limited investigation has been available on the effects of dietary phospholipids in leopard coral grouper (<em>Plectropomus leopardus</em>), hindering its potential for aquaculture. This study was intended to investigate the impact of various levels (0<img>5 %) of dietary soy lecithin (SL) on juvenile <em>P. leopardus</em> over a 9-week feeding trial. Results indicated that dietary inclusion of 2<img>3 % SL enhanced the lipid content in liver, muscle, and whole-body. The fatty acid composition of muscle strongly reflected the dietary fatty acid profile. Furthermore, dietary inclusion of 3 % SL enhanced the activities of hepatic lipid synthesis enzymes [fatty acid synthase (FAS), glucose-6-phosphate dehydrogenase (G6PD)] and lipolytic enzymes [hormone-sensitive lipase (HSL), carnitine palmitoyltransferase-1 (CPT-1)]. Additionally, the inclusion of 2<img>3 % SL upregulated the relative expression of lipid synthesis-related genes [<em>fas</em>, sterol regulatory element binding transcription factor 1 (<em>srebp1</em>)] and lipid catabolism-related genes [<em>hsl</em>, <em>cpt-1</em>, and lipoprotein lipase (<em>lpl</em>)] in muscle. However, increasing dietary SL levels up to 4<img>5 % led to a reduction in muscle and whole-body lipid content, and an enhance in hepatic lipid content and hepatocyte vacuolization. Moreover, dietary inclusion of 5 % SL downregulated the relative expressions of hepatic lipid synthesis-related genes (<em>g6pd</em> and <em>srebp1</em>) and lipid catabolism-related genes (<em>lpl</em> and <em>hsl</em>). Overall, including 2<img>3 % SL in the diet improved lipid metabolism and thereby enhanced lipid deposition in juvenile <em>P. leopardus</em>, but excessive SL (4<img>5 %) disrupted lipid metabolism.</p></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":null,"pages":null},"PeriodicalIF":3.2000,"publicationDate":"2024-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352513424003533/pdfft?md5=ecd678dbd61a76b62c9a0fcbbe87ae98&pid=1-s2.0-S2352513424003533-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquaculture Reports","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352513424003533","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FISHERIES","Score":null,"Total":0}
引用次数: 0
Abstract
Limited investigation has been available on the effects of dietary phospholipids in leopard coral grouper (Plectropomus leopardus), hindering its potential for aquaculture. This study was intended to investigate the impact of various levels (05 %) of dietary soy lecithin (SL) on juvenile P. leopardus over a 9-week feeding trial. Results indicated that dietary inclusion of 23 % SL enhanced the lipid content in liver, muscle, and whole-body. The fatty acid composition of muscle strongly reflected the dietary fatty acid profile. Furthermore, dietary inclusion of 3 % SL enhanced the activities of hepatic lipid synthesis enzymes [fatty acid synthase (FAS), glucose-6-phosphate dehydrogenase (G6PD)] and lipolytic enzymes [hormone-sensitive lipase (HSL), carnitine palmitoyltransferase-1 (CPT-1)]. Additionally, the inclusion of 23 % SL upregulated the relative expression of lipid synthesis-related genes [fas, sterol regulatory element binding transcription factor 1 (srebp1)] and lipid catabolism-related genes [hsl, cpt-1, and lipoprotein lipase (lpl)] in muscle. However, increasing dietary SL levels up to 45 % led to a reduction in muscle and whole-body lipid content, and an enhance in hepatic lipid content and hepatocyte vacuolization. Moreover, dietary inclusion of 5 % SL downregulated the relative expressions of hepatic lipid synthesis-related genes (g6pd and srebp1) and lipid catabolism-related genes (lpl and hsl). Overall, including 23 % SL in the diet improved lipid metabolism and thereby enhanced lipid deposition in juvenile P. leopardus, but excessive SL (45 %) disrupted lipid metabolism.
Aquaculture ReportsAgricultural and Biological Sciences-Animal Science and Zoology
CiteScore
5.90
自引率
8.10%
发文量
469
审稿时长
77 days
期刊介绍:
Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.