Aquaculture Reports最新文献

Effects of dietary curcumin supplementation on growth performance, antioxidant capacity, and gene transcriptome in the juvenile Manila clam (Ruditapes philippinarum)

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2025-03-06 DOI: 10.1016/j.aqrep.2025.102727

Yang Zhang , Hailong Huang , Kai Liao , Yuxiang Zhu , Yang Liu , Deshui Chen , Bin Ma , Hui Ge , Jilin Xu

The complex conditions in aquaculture environments often lead to oxidative stress in juvenile bivalves, highlighting the need for effective mitigation strategies. This study evaluated the effects of dietary curcumin supplementation on growth performance, antioxidant capacity, and gene transcriptome in the juvenile clam (Ruditapes philippinarum). A total of 36 g of clams (individual body weight: 0.5 ± 0.17 mg, shell length: 1.15 ± 0.15 mm) were equally distributed into six groups (three replicates per group) and fed six microencapsulated diets containing 0, 50, 100, 200, 400, and 800 mg/kg of curcumin for three weeks. Results indicated that curcumin supplementation did not significantly influence growth performance. However, clams in the 200 mg/kg and 400 mg/kg groups exhibited markedly higher activities of catalase, superoxide dismutase (SOD), and glutathione, along with reduced malondialdehyde (MDA) levels compared to the 0 mg/kg group. Following treatment with hydrogen peroxide, the survival rate of clams in 400 mg/kg group was significantly higher than 0 mg/kg group. Meanwhile, the activities of SOD and Glutathione Peroxidase were significantly increased in the 400 mg/kg group, with a corresponding reduction in MDA levels compared to the 0 mg/kg group. The transcriptome analysis further revealed that supplementation with 400 mg/kg curcumin significantly upregulated the expression of genes related to autophagy and lysosomal pathways when compared to the 0 mg/kg group. In conclusion, our study suggested that supplementation with 400 mg/kg curcumin significantly enhanced the antioxidant capacity of clams, providing essential insights for mitigating oxidative stress in juvenile bivalve aquaculture.

{"title":"Effects of dietary curcumin supplementation on growth performance, antioxidant capacity, and gene transcriptome in the juvenile Manila clam (Ruditapes philippinarum)","authors":"Yang Zhang , Hailong Huang , Kai Liao , Yuxiang Zhu , Yang Liu , Deshui Chen , Bin Ma , Hui Ge , Jilin Xu","doi":"10.1016/j.aqrep.2025.102727","DOIUrl":"10.1016/j.aqrep.2025.102727","url":null,"abstract":"<div><div>The complex conditions in aquaculture environments often lead to oxidative stress in juvenile bivalves, highlighting the need for effective mitigation strategies. This study evaluated the effects of dietary curcumin supplementation on growth performance, antioxidant capacity, and gene transcriptome in the juvenile clam (<em>Ruditapes philippinarum</em>). A total of 36 g of clams (individual body weight: 0.5 ± 0.17 mg, shell length: 1.15 ± 0.15 mm) were equally distributed into six groups (three replicates per group) and fed six microencapsulated diets containing 0, 50, 100, 200, 400, and 800 mg/kg of curcumin for three weeks. Results indicated that curcumin supplementation did not significantly influence growth performance. However, clams in the 200 mg/kg and 400 mg/kg groups exhibited markedly higher activities of catalase, superoxide dismutase (SOD), and glutathione, along with reduced malondialdehyde (MDA) levels compared to the 0 mg/kg group. Following treatment with hydrogen peroxide, the survival rate of clams in 400 mg/kg group was significantly higher than 0 mg/kg group. Meanwhile, the activities of SOD and Glutathione Peroxidase were significantly increased in the 400 mg/kg group, with a corresponding reduction in MDA levels compared to the 0 mg/kg group. The transcriptome analysis further revealed that supplementation with 400 mg/kg curcumin significantly upregulated the expression of genes related to autophagy and lysosomal pathways when compared to the 0 mg/kg group. In conclusion, our study suggested that supplementation with 400 mg/kg curcumin significantly enhanced the antioxidant capacity of clams, providing essential insights for mitigating oxidative stress in juvenile bivalve aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102727"},"PeriodicalIF":3.2,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of hydroxyproline-taurine-cholesterol supplementation on the liver health and lipid metabolisms of turbot Scophthalmus maximus fed Chlorella vulgaris

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2025-03-05 DOI: 10.1016/j.aqrep.2025.102726

Daisong Ma , Nan Bai , Shihui Pan , Chuwen Chen , Min Gu

Microalgae, such as Chlorella vulgaris (CV), possess significant potential as a protein source for aquafeed. However, it has been demonstrated that CV can cause liver damage in carnivorous fish. The present study was aimed at evaluating a combination of hydroxyproline, taurine, and cholesterol (HTC, in a ratio of 1:1:2) for the purpose of alleviating this issue. Seven experimental diets were formulated. The control diet utilized fishmeal as the exclusive protein source. Three CV diets were designed, in which CV substituted 45 %, 60 %, or 75 % of the fishmeal protein. Moreover, three HTC diets were prepared by incorporating 2 % of HTC into the CV diets. Each of the seven diets was fed to three groups of turbot with an initial average weight of 211.6 ± 1.0 g. The results showed that CV at any assayed level reduced the growth performance and feed intake of turbot and induced inflammatory damage to the liver. CV also disrupted lipid metabolism, as indicated by the significantly lower levels of total triglyceride and total cholesterol in the plasma and bile acids in the liver (P < 0.05). The expression of cyp7α1, which encodes the rate-limiting enzyme for bile acid synthesis, was significantly reduced by CV (P < 0.05). Concurrent supplementation of HTC with CV completely restored bile acid metabolism. The bile acid levels and the expression of cyp7α1 in all HTC groups were at the same level as those in the control group (P > 0.05). HTC also increased the total triglyceride and total cholesterol levels in the plasma while reducing the liver lipid content. Moreover, HTC effectively reduced liver injury by alleviating the inflammatory condition and down -regulating the expression of inflammatory-related cytokines induced by CV. In conclusion, the present study provides evidence that the combination of hydroxyproline, taurine, and cholesterol has a hepatic protection function, possibly due to its ability to restore the bile acid status disturbed by CV.

{"title":"Effects of hydroxyproline-taurine-cholesterol supplementation on the liver health and lipid metabolisms of turbot Scophthalmus maximus fed Chlorella vulgaris","authors":"Daisong Ma , Nan Bai , Shihui Pan , Chuwen Chen , Min Gu","doi":"10.1016/j.aqrep.2025.102726","DOIUrl":"10.1016/j.aqrep.2025.102726","url":null,"abstract":"<div><div>Microalgae, such as <em>Chlorella vulgaris</em> (CV), possess significant potential as a protein source for aquafeed. However, it has been demonstrated that CV can cause liver damage in carnivorous fish. The present study was aimed at evaluating a combination of hydroxyproline, taurine, and cholesterol (HTC, in a ratio of 1:1:2) for the purpose of alleviating this issue. Seven experimental diets were formulated. The control diet utilized fishmeal as the exclusive protein source. Three CV diets were designed, in which CV substituted 45 %, 60 %, or 75 % of the fishmeal protein. Moreover, three HTC diets were prepared by incorporating 2 % of HTC into the CV diets. Each of the seven diets was fed to three groups of turbot with an initial average weight of 211.6 ± 1.0 g. The results showed that CV at any assayed level reduced the growth performance and feed intake of turbot and induced inflammatory damage to the liver. CV also disrupted lipid metabolism, as indicated by the significantly lower levels of total triglyceride and total cholesterol in the plasma and bile acids in the liver (<em>P</em> < 0.05). The expression of <em>cyp7α1</em>, which encodes the rate-limiting enzyme for bile acid synthesis, was significantly reduced by CV (<em>P</em> < 0.05). Concurrent supplementation of HTC with CV completely restored bile acid metabolism. The bile acid levels and the expression of <em>cyp7α1</em> in all HTC groups were at the same level as those in the control group (<em>P</em> > 0.05). HTC also increased the total triglyceride and total cholesterol levels in the plasma while reducing the liver lipid content. Moreover, HTC effectively reduced liver injury by alleviating the inflammatory condition and down -regulating the expression of inflammatory-related cytokines induced by CV. In conclusion, the present study provides evidence that the combination of hydroxyproline, taurine, and cholesterol has a hepatic protection function, possibly due to its ability to restore the bile acid status disturbed by CV.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102726"},"PeriodicalIF":3.2,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic analysis of the toxic effects and potential mechanisms of fenvalerate on the liver of Odontobutis potamophila

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2025-03-04 DOI: 10.1016/j.aqrep.2025.102721

Guoxing Liu , Tian Zhu , Zihan Zhou , You Zheng , Yanhua Zhao , Chen Hao , Shuyan Ding , Xiuhui Tan , Qichen Jiang

This study explored the toxic effects of short-term exposure (96 h) to fenvalerate (FEN) at environmental concentrations (C0.5: 0.5 µg/L) and application concentrations (C2: 2 µg/L) on the liver of Odontobutis potamophila and analyzed the potential toxicity mechanisms. The results showed that FEN exposure caused tissue damage and oxidative stress in O. potamophila liver in a concentration-dependent manner. Transcriptomic analysis revealed 2083 differentially expressed genes (DEGs) between control (CK) and C0.5 treatments and 2595 DEGs between CK and C2 treatments. Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed significant DEG enrichment in pathways such as serotonergic synapse, estrogen signaling, retrograde endocannabinoid signaling, etc. in C0.5, and in pathways such as oxidative phosphorylation, glutathione metabolism, retrograde endocannabinoid signaling, etc. in C2. Gene Ontology enrichment analysis showed significant DEGs enrichment in pathways such as DNA integration, obsolete oxidation-reduction process, regulation of DNA-templated transcription, etc. in C0.5, and in pathways such as obsolete oxidation-reduction process, translation, structural constituent of ribosome, etc. in C2. Investigation of genes related to liver mitochondrial function, including expression of cytochrome b (CYTB), cytochrome c oxidase subunit II (COX2), cytochrome c oxidase subunit III (COX3), and NADH dehydrogenase subunit 1 (ND1), demonstrated that CYTB and ND1 were significantly downregulated (P < 0.05) in a concentration-dependent manner, while COX2 and COX3 were significantly upregulated (P < 0.05) in C0.5 and C2, indicating that FEN could damage mitochondrial function in O. potamophila liver. In summary, this study revealed that the toxic effects of FEN on the liver of O. potamophila manifested as causing oxidative stress, resulting in tissue damage and mitochondrial dysfunction, and its key pathway of action is retrograde endocannabinoid signaling pathway. This results provide a theoretical basis for research on the toxic effects of FEN on aquatic organisms, and assessed the environmental risks of FEN exposure.

{"title":"Transcriptomic analysis of the toxic effects and potential mechanisms of fenvalerate on the liver of Odontobutis potamophila","authors":"Guoxing Liu , Tian Zhu , Zihan Zhou , You Zheng , Yanhua Zhao , Chen Hao , Shuyan Ding , Xiuhui Tan , Qichen Jiang","doi":"10.1016/j.aqrep.2025.102721","DOIUrl":"10.1016/j.aqrep.2025.102721","url":null,"abstract":"<div><div>This study explored the toxic effects of short-term exposure (96 h) to fenvalerate (FEN) at environmental concentrations (C0.5: 0.5 µg/L) and application concentrations (C2: 2 µg/L) on the liver of <em>Odontobutis potamophila</em> and analyzed the potential toxicity mechanisms. The results showed that FEN exposure caused tissue damage and oxidative stress in <em>O. potamophila</em> liver in a concentration-dependent manner. Transcriptomic analysis revealed 2083 differentially expressed genes (DEGs) between control (CK) and C0.5 treatments and 2595 DEGs between CK and C2 treatments. Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed significant DEG enrichment in pathways such as serotonergic synapse, estrogen signaling, retrograde endocannabinoid signaling, etc. in C0.5, and in pathways such as oxidative phosphorylation, glutathione metabolism, retrograde endocannabinoid signaling, etc. in C2. Gene Ontology enrichment analysis showed significant DEGs enrichment in pathways such as DNA integration, obsolete oxidation-reduction process, regulation of DNA-templated transcription, etc. in C0.5, and in pathways such as obsolete oxidation-reduction process, translation, structural constituent of ribosome, etc. in C2. Investigation of genes related to liver mitochondrial function, including expression of cytochrome b (<em>CYTB</em>), cytochrome c oxidase subunit II (<em>COX2</em>), cytochrome c oxidase subunit III (<em>COX3</em>), and NADH dehydrogenase subunit 1 (<em>ND1</em>), demonstrated that <em>CYTB</em> and <em>ND1</em> were significantly downregulated (<em>P</em> < 0.05) in a concentration-dependent manner, while <em>COX2</em> and <em>COX3</em> were significantly upregulated (<em>P</em> < 0.05) in C0.5 and C2, indicating that FEN could damage mitochondrial function in <em>O. potamophila</em> liver. In summary, this study revealed that the toxic effects of FEN on the liver of O<em>. potamophila</em> manifested as causing oxidative stress, resulting in tissue damage and mitochondrial dysfunction, and its key pathway of action is retrograde endocannabinoid signaling pathway. This results provide a theoretical basis for research on the toxic effects of FEN on aquatic organisms, and assessed the environmental risks of FEN exposure.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102721"},"PeriodicalIF":3.2,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimal feeding levels to enhance growth performance and gut microbiota balance in red swamp crayfish (Procambarus clarkii)

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2025-03-03 DOI: 10.1016/j.aqrep.2025.102717

Xuhong Yang , Zhanyang Tang , Kai Huang , Ruijie Guo , Dandan Wang , Shisong Jiang , Kai Yu

This study examined the impact of various feeding levels on the growth performance and intestinal microbiota composition of Procambarus clarkii. A total of 540 juvenile crayfish (initial weight: 5.95 ± 0.25 g) were allocated to six experimental groups (1 %, 2 %, 3 %, 4 %, 5 %, and 6 % of body weight), with three replicates per group (30 crayfish per replicate), and cultured for 45 days. Growth parameters, physiological indices, gene expression, and intestinal microbiota were assessed to identity the optimal feeding level. The results revealed that feeding level significantly influenced growth outcomes. The FL4 group (4.48 % feeding level) demonstrated the highest flesh content (FC), survival rate (SR), and overall growth performance. Both excessively high and low feeding levels impaired antioxidant capacity (SOD, CAT), immune function (ACP, AKP, LZM), and induced liver damage (increased AST and ALT) and oxidative stress (elevated MDA). Additionally, feeding level modulated liver digestive enzyme activity, with FL4 exhibiting the most favorable biochemical profile. Gene expression analysis highlighted that TGF-β and Mstn were inversely correlated with growth, with the lowest expression observed in FL4. Conversely, growth-related genes (ACTL, TNT, MCL, MHC) were most highly expressed in FL4. The molt-related gene MIH showed a negative correlation with feeding level, whereas E75, EcR, and RXR were optimally expressed in FL4. Intestinal microbiota analysis indicated that feeding level significantly impacted microbial diversity and abundance. Higher feeding levels promoted an increase in Bacteroidota, while Firmicutes and the Firmicutes/Bacteroidetes ratio were negatively correlated with feeding levels. Lower feeding levels favored the growth of potential pathogens, such as Aeromonas, Shewanella, and Tyzzerella, which were negatively correlated with both growth and molt-related gene expression. In conclusion, a feeding level of 4.48 % optimized growth performance andmaintained a balanced intestinal microbiota.

{"title":"Optimal feeding levels to enhance growth performance and gut microbiota balance in red swamp crayfish (Procambarus clarkii)","authors":"Xuhong Yang , Zhanyang Tang , Kai Huang , Ruijie Guo , Dandan Wang , Shisong Jiang , Kai Yu","doi":"10.1016/j.aqrep.2025.102717","DOIUrl":"10.1016/j.aqrep.2025.102717","url":null,"abstract":"<div><div>This study examined the impact of various feeding levels on the growth performance and intestinal microbiota composition of <em>Procambarus clarkii</em>. A total of 540 juvenile crayfish (initial weight: 5.95 ± 0.25 g) were allocated to six experimental groups (1 %, 2 %, 3 %, 4 %, 5 %, and 6 % of body weight), with three replicates per group (30 crayfish per replicate), and cultured for 45 days. Growth parameters, physiological indices, gene expression, and intestinal microbiota were assessed to identity the optimal feeding level. The results revealed that feeding level significantly influenced growth outcomes. The FL4 group (4.48 % feeding level) demonstrated the highest flesh content (FC), survival rate (SR), and overall growth performance. Both excessively high and low feeding levels impaired antioxidant capacity (SOD, CAT), immune function (ACP, AKP, LZM), and induced liver damage (increased AST and ALT) and oxidative stress (elevated MDA). Additionally, feeding level modulated liver digestive enzyme activity, with FL4 exhibiting the most favorable biochemical profile. Gene expression analysis highlighted that <em>TGF-β</em> and <em>Mstn</em> were inversely correlated with growth, with the lowest expression observed in FL4. Conversely, growth-related genes (<em>ACTL</em>, <em>TNT</em>, <em>MCL</em>, <em>MHC</em>) were most highly expressed in FL4. The molt-related gene <em>MIH</em> showed a negative correlation with feeding level, whereas <em>E75</em>, <em>EcR</em>, and <em>RXR</em> were optimally expressed in FL4. Intestinal microbiota analysis indicated that feeding level significantly impacted microbial diversity and abundance. Higher feeding levels promoted an increase in Bacteroidota, while Firmicutes and the Firmicutes/Bacteroidetes ratio were negatively correlated with feeding levels. Lower feeding levels favored the growth of potential pathogens, such as <em>Aeromonas</em>, <em>Shewanella</em>, and <em>Tyzzerella</em>, which were negatively correlated with both growth and molt-related gene expression. In conclusion, a feeding level of 4.48 % optimized growth performance andmaintained a balanced intestinal microbiota.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"41 ","pages":"Article 102717"},"PeriodicalIF":3.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA methylation provides new insights into the epigenetic regulation of Vibrio parahaemolyticus infection in Portunus trituberculatus

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2025-03-03 DOI: 10.1016/j.aqrep.2025.102693

Dongfang Sun , Xianfa Zhou , Shaoting Jia , Ping Liu , Jianjian Lv

Vibrio parahaemolyticus is the primary pathogen responsible for acute hepatopancreatic necrotic disease in crustaceans. Research on pathogenic mechanism of V. parahaemolyticus in crustaceans has predominantly concentrated on genetics, with limited exploration of epigenetic regulatory mechanisms. Here, to elucidate the epigenetic regulatory mechanisms of crustacean resistance to V. parahaemolyticus infection, we conducted an artificial infection study on P. trituberculatus. The results indicated a significant increase in the expression of DNA methyltransferase Dnmt1 following 72 h of V. parahaemolyticus infection. Subsequently, we depict for the first time DNA methylation profiles of the whole genome of P. trituberculatus at single-base resolution by using WGBS on hepatopancreatic tissues. The overall DNA methylation level was low at 2.2 % in P. trituberculatus. Methylation sites exhibited a mosaic distribution across the genome, predominantly of the CG type, in line with other invertebrates. The DNA methylation of P. trituberculatus changed significantly after pathogen infection, showing localized hypermethylation or hypomethylation. A total of 1832 DMRs, with 1072 being hypermethylated and 766 hypomethylated were identified. The DMRs were annotated across 1005 genes. Differently methylated genes were significantly enriched in immune-related signaling pathways, including enrichment in the NF-kappa β signaling pathway and platelet activation. Finally, we verified the inhibitory effect of DNA methylation on the expression of the immune-related gene Dad1 using a dual luciferase reporter assay. These findings will enhance our understanding of the regulatory mechanisms in crustacean innate immunity and offer epigenetic markers for breeding disease-resistant P. trituberculatus varieties, presenting significant theoretical and practical value.

{"title":"DNA methylation provides new insights into the epigenetic regulation of Vibrio parahaemolyticus infection in Portunus trituberculatus","authors":"Dongfang Sun , Xianfa Zhou , Shaoting Jia , Ping Liu , Jianjian Lv","doi":"10.1016/j.aqrep.2025.102693","DOIUrl":"10.1016/j.aqrep.2025.102693","url":null,"abstract":"<div><div><em>Vibrio parahaemolyticus</em> is the primary pathogen responsible for acute hepatopancreatic necrotic disease in crustaceans. Research on pathogenic mechanism of <em>V. parahaemolyticus</em> in crustaceans has predominantly concentrated on genetics, with limited exploration of epigenetic regulatory mechanisms. Here, to elucidate the epigenetic regulatory mechanisms of crustacean resistance to <em>V. parahaemolyticus</em> infection, we conducted an artificial infection study on <em>P. trituberculatus</em>. The results indicated a significant increase in the expression of DNA methyltransferase <em>Dnmt1</em> following 72 h of <em>V. parahaemolyticus</em> infection. Subsequently, we depict for the first time DNA methylation profiles of the whole genome of <em>P. trituberculatus</em> at single-base resolution by using WGBS on hepatopancreatic tissues. The overall DNA methylation level was low at 2.2 % in <em>P. trituberculatus</em>. Methylation sites exhibited a mosaic distribution across the genome, predominantly of the CG type, in line with other invertebrates. The DNA methylation of <em>P. trituberculatus</em> changed significantly after pathogen infection, showing localized hypermethylation or hypomethylation. A total of 1832 DMRs, with 1072 being hypermethylated and 766 hypomethylated were identified. The DMRs were annotated across 1005 genes. Differently methylated genes were significantly enriched in immune-related signaling pathways, including enrichment in the NF-kappa β signaling pathway and platelet activation. Finally, we verified the inhibitory effect of DNA methylation on the expression of the immune-related gene <em>Dad1</em> using a dual luciferase reporter assay. These findings will enhance our understanding of the regulatory mechanisms in crustacean innate immunity and offer epigenetic markers for breeding disease-resistant <em>P. trituberculatus</em> varieties, presenting significant theoretical and practical value.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"41 ","pages":"Article 102693"},"PeriodicalIF":3.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the effect of dietary nano selenium and/or Vitamin E and their interplay on reproductive physiology of Nile tilapia broodstock: Insights into spawning performance, haemato-biochemical profile, gene expression and gonadal architecture.

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2025-03-03 DOI: 10.1016/j.aqrep.2025.102722

Sara F. Ghanem , Muna Omer Alamoudi , Moaheda E.H. Eissa , Roshmon Thomas Mathew , Bassam A. Alahmadi , El-Sayed Hemdan Eissa , Heba E. Abd Elnabi

This research was conducted to explore the dietary effects of selenium nanoparticles (NSe), Vitamin E (VE) and their mixture supplementation on reproduction capacity, haemato-biochemical responses, spawning performance, gene expression and gonadal histology of Nile tilapia (Oreochromis. niloticus) broodstock. Fish brooders were allocated into four treatments in triplicates and served the formulated diets: basal diet (Control, G₀), 1 mg NSe kg⁻¹ (G₁),100 mg VE kg⁻¹ (G₂) and an inclusion of 1 mg NSe kg⁻¹ + 100 mg VE kg⁻¹ (G₃). Diets were fed to Nile tilapia (215 ± 34.56 g) stocked at a male: female ratio of 1: 3 for 60 days. The results revealed that hematological parameters (RBCs, WBCs, PCV, Hb), total protein, globulin and albumin were significantly increased (P < 0.05) in fish group fed dietary combination of 1 mg NSe kg⁻¹ + 100 mg VE kg⁻¹. While other indices (MCV, MCH, MCHC) remained unaffected among the different treated groups (P > 0.05). Optimum liver function enzyme activity and the highest reproductive hormones (MT, FSH, LH and progesterone) concentrations were found in the dietary inclusion of 1 mg NSe kg⁻¹ + 100 mg VE kg⁻¹. Organo-somatic indices, egg diameter and expression of reproductive genes (vasa, nanos1a, nanos2, dnd1, pum1, amh and VTG) are boosted (P < 0.05) in 1 mg NSe kg⁻¹ + 100 mg VE kg⁻¹ showing that this blend is required for the optimal reproductive capacity. Reproductive performance (mean fry number per female and mean fry weight) increased and gonadal architecture were improved in NSe-supplemented groups, with the highest recorded level in fish fed NSe/VE blend. In conclusion, our results revealed that dietary supplementation with 1 mg NSe kg⁻¹ and/or 100 mg VE kg⁻¹ can be effectively used for reproductive performance of Nile tilapia. The inclusion of NSe and VE at 1:100 mg/kg incorporated into fish diet seems to have a synergistic effect required for supporting haemato-biochemical indices and enhancing the overall spawning performance of Nile tilapia broodstock by increasing concentrations of sexual hormones, improving egg diameter, boosting gonadal puberty and up-regulating the expression of reproductive-related genes.

{"title":"Exploring the effect of dietary nano selenium and/or Vitamin E and their interplay on reproductive physiology of Nile tilapia broodstock: Insights into spawning performance, haemato-biochemical profile, gene expression and gonadal architecture.","authors":"Sara F. Ghanem , Muna Omer Alamoudi , Moaheda E.H. Eissa , Roshmon Thomas Mathew , Bassam A. Alahmadi , El-Sayed Hemdan Eissa , Heba E. Abd Elnabi","doi":"10.1016/j.aqrep.2025.102722","DOIUrl":"10.1016/j.aqrep.2025.102722","url":null,"abstract":"<div><div>This research was conducted to explore the dietary effects of selenium nanoparticles (NSe), Vitamin E (VE) and their mixture supplementation on reproduction capacity, haemato-biochemical responses, spawning performance, gene expression and gonadal histology of Nile tilapia (<em>Oreochromis. niloticus</em>) broodstock. Fish brooders were allocated into four treatments in triplicates and served the formulated diets: basal diet (Control, G<sub>0</sub>), 1 mg NSe kg<sup>−1</sup> (G<sub>1</sub>),100 mg VE kg<sup>−1</sup> (G<sub>2</sub>) and an inclusion of 1 mg NSe kg<sup>−1</sup> + 100 mg VE kg<sup>−1</sup> (G<sub>3</sub>). Diets were fed to Nile tilapia (215 ± 34.56 g) stocked at a male: female ratio of 1: 3 for 60 days. The results revealed that hematological parameters (RBCs, WBCs, PCV, Hb), total protein, globulin and albumin were significantly increased (<em>P < 0.05</em>) in fish group fed dietary combination of 1 mg NSe kg<sup>−1</sup> + 100 mg VE kg<sup>−1</sup>. While other indices (MCV, MCH, MCHC) remained unaffected among the different treated groups (<em>P</em> > 0.05). Optimum liver function enzyme activity and the highest reproductive hormones (MT, FSH, LH and progesterone) concentrations were found in the dietary inclusion of 1 mg NSe kg<sup>−1</sup> + 100 mg VE kg<sup>−1</sup>. Organo-somatic indices, egg diameter and expression of reproductive genes (<em>vasa</em>, <em>nanos1a, nanos2, dnd1, pum1, amh</em> and <em>VTG</em>) are boosted (<em>P < 0.05</em>) in 1 mg NSe kg<sup>−1</sup> + 100 mg VE kg<sup>−1</sup> showing that this blend is required for the optimal reproductive capacity. Reproductive performance (mean fry number per female and mean fry weight) increased and gonadal architecture were improved in NSe-supplemented groups, with the highest recorded level in fish fed NSe/VE blend. In conclusion, our results revealed that dietary supplementation with 1 mg NSe kg<sup>−1</sup> and/or 100 mg VE kg<sup>−1</sup> can be effectively used for reproductive performance of Nile tilapia. The inclusion of NSe and VE at 1:100 mg/kg incorporated into fish diet seems to have a synergistic effect required for supporting haemato-biochemical indices and enhancing the overall spawning performance of Nile tilapia broodstock by increasing concentrations of sexual hormones, improving egg diameter, boosting gonadal puberty and up-regulating the expression of reproductive-related genes.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"41 ","pages":"Article 102722"},"PeriodicalIF":3.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing Nile Tilapia growth and production costs in earthen ponds using multi-objective adaptive artificial intelligence systems

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2025-03-03 DOI: 10.1016/j.aqrep.2025.102716

Keartisak Sriprateep , Rapeepan Pitakaso , Surajet Khonjun , Peerawat Luesak , Achara Jutagate , Chutchai Kaewta , Thanatkij Srichok , Monika Kosacka-Olejnik , Surasak Matitopanum

The rising global demand for Nile tilapia necessitates innovative production strategies to address food security challenges and drive economic growth in aquaculture. Optimizing tilapia yields, especially in earthen ponds, is crucial but often hindered by inefficiencies like overfeeding and suboptimal stocking densities, escalating costs and environmental concerns. Traditional aquaculture approaches frequently fail to balance operational expenditures, environmental sustainability, and fish health due to an overreliance on single-factor analyses, overlooking the complex interplay between multiple variables. This study introduces a novel multi-objective optimization approach by integrating the Design of Experiments (DOE) method with the Adaptive Artificial Multiple Intelligence System (AAMIS) to optimize aquaculture parameters. The Taguchi DOE method efficiently designs experiments by selecting essential variables and their levels. Multi-objective regression models developed using statistical software and fine-tuned by AAMIS, balance maximizing yield with minimizing costs. Computational analysis validates AAMIS's superior performance, achieving leading Hypervolume and Average Ratio of Pareto Optimal Solutions scores, surpassing traditional and advanced methods. AAMIS reduces life cycle costs to $39,880 and achieves the lowest environmental impact score of 3460.76, setting new benchmarks for sustainable aquaculture practices. This approach demonstrates broad applicability across diverse aquaculture systems, promising global improvements in sustainability and efficiency.

{"title":"Optimizing Nile Tilapia growth and production costs in earthen ponds using multi-objective adaptive artificial intelligence systems","authors":"Keartisak Sriprateep , Rapeepan Pitakaso , Surajet Khonjun , Peerawat Luesak , Achara Jutagate , Chutchai Kaewta , Thanatkij Srichok , Monika Kosacka-Olejnik , Surasak Matitopanum","doi":"10.1016/j.aqrep.2025.102716","DOIUrl":"10.1016/j.aqrep.2025.102716","url":null,"abstract":"<div><div>The rising global demand for Nile tilapia necessitates innovative production strategies to address food security challenges and drive economic growth in aquaculture. Optimizing tilapia yields, especially in earthen ponds, is crucial but often hindered by inefficiencies like overfeeding and suboptimal stocking densities, escalating costs and environmental concerns. Traditional aquaculture approaches frequently fail to balance operational expenditures, environmental sustainability, and fish health due to an overreliance on single-factor analyses, overlooking the complex interplay between multiple variables. This study introduces a novel multi-objective optimization approach by integrating the Design of Experiments (DOE) method with the Adaptive Artificial Multiple Intelligence System (AAMIS) to optimize aquaculture parameters. The Taguchi DOE method efficiently designs experiments by selecting essential variables and their levels. Multi-objective regression models developed using statistical software and fine-tuned by AAMIS, balance maximizing yield with minimizing costs. Computational analysis validates AAMIS's superior performance, achieving leading Hypervolume and Average Ratio of Pareto Optimal Solutions scores, surpassing traditional and advanced methods. AAMIS reduces life cycle costs to $39,880 and achieves the lowest environmental impact score of 3460.76, setting new benchmarks for sustainable aquaculture practices. This approach demonstrates broad applicability across diverse aquaculture systems, promising global improvements in sustainability and efficiency.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"41 ","pages":"Article 102716"},"PeriodicalIF":3.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of Triclosan (TCS) on the survival, serum biomarkers, antioxidant defense, and apoptosis of mud crab (Scylla paramamosain)

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2025-03-01 DOI: 10.1016/j.aqrep.2025.102719

Yue Wang , Ting-Ting Hou , Xue-Feng Song , Zhi-Xing Su , Jia-Yuan Xu , Xiao-Kang Lv , Shuai Zhou , Bian-Bian Zhang , Yan-Ming Sui , Li-Guo Yang

Triclosan (TCS), a widely used antimicrobial agent, is known to have toxic effects on aquatic organisms, potentially disrupting reproduction, development, and behavior. However, its impact on marine invertebrates, particularly crustaceans, remains understudied. To evaluate the effects of TCS, juvenile mud crabs (Scylla paramamosain) were exposed to TCS concentrations ranging from 50 to 5000 μg/L over a 96-hour period in this study. Results showed that low TCS concentrations had no significant short-term effects on the crabs, whereas moderate to high concentrations caused disruptions in hemolymph parameters, increased oxidative stress, and reduced antioxidant enzyme activity, ultimately leading to mortality. Exposure to these higher concentrations also led to the upregulation of heat shock proteins (HSPs) and antioxidant enzyme mRNA expression as part of the stress response, alongside the increased expression of pro-apoptotic genes and suppression of anti-apoptotic genes. The effects were generally similar between sexes, although females showed greater susceptibility than males. Additionally, TCS exposure altered serum cholesterol levels in females but had no effect on males, suggesting a possible estrogenic effect of TCS that requires further investigation. This study provides important data for assessing the risks of TCS in aquatic environments and has significant implications for the conservation of estuarine ecosystems and fisheries resources.

{"title":"The effects of Triclosan (TCS) on the survival, serum biomarkers, antioxidant defense, and apoptosis of mud crab (Scylla paramamosain)","authors":"Yue Wang , Ting-Ting Hou , Xue-Feng Song , Zhi-Xing Su , Jia-Yuan Xu , Xiao-Kang Lv , Shuai Zhou , Bian-Bian Zhang , Yan-Ming Sui , Li-Guo Yang","doi":"10.1016/j.aqrep.2025.102719","DOIUrl":"10.1016/j.aqrep.2025.102719","url":null,"abstract":"<div><div>Triclosan (TCS), a widely used antimicrobial agent, is known to have toxic effects on aquatic organisms, potentially disrupting reproduction, development, and behavior. However, its impact on marine invertebrates, particularly crustaceans, remains understudied. To evaluate the effects of TCS, juvenile mud crabs (<em>Scylla paramamosain</em>) were exposed to TCS concentrations ranging from 50 to 5000 μg/L over a 96-hour period in this study. Results showed that low TCS concentrations had no significant short-term effects on the crabs, whereas moderate to high concentrations caused disruptions in hemolymph parameters, increased oxidative stress, and reduced antioxidant enzyme activity, ultimately leading to mortality. Exposure to these higher concentrations also led to the upregulation of heat shock proteins (HSPs) and antioxidant enzyme mRNA expression as part of the stress response, alongside the increased expression of pro-apoptotic genes and suppression of anti-apoptotic genes. The effects were generally similar between sexes, although females showed greater susceptibility than males. Additionally, TCS exposure altered serum cholesterol levels in females but had no effect on males, suggesting a possible estrogenic effect of TCS that requires further investigation. This study provides important data for assessing the risks of TCS in aquatic environments and has significant implications for the conservation of estuarine ecosystems and fisheries resources.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"41 ","pages":"Article 102719"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143518951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation between SNPs in promoter region of gene, L-Type lectin (LvLTLC1) and the resistance trait of shrimp, Litopenaeus vannamei, against vibrios

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2025-03-01 DOI: 10.1016/j.aqrep.2025.102725

Shuyang Wen , Lihong Yuan , Jiayue Yin , Chaoqun Hu , Xin Zhang , Yunyi Huang , Chujie Zhao , Liyan Liu , Peng Luo

Pacific white shrimp, Litopenaeus vannamei (L. vannamei), is the most economically valuable shrimp species in world. Bacterial diseases mainly caused by various vibrios bring huge economic loss in shrimp aquaculture. Previous research has confirmed that LvLTLC1 gene coding a L-type lectin of L. vannamei plays a pivotal role in the resistance of shrimp against pathogenic vibrios, which implies the potential of LvLTLC1 as a target gene to develop SNP markers for genetic breeding of the shrimp. In this study, pan-vibrio resistance (PVR) trait of L. vannamei was quantitatively evaluated by vibrios loads in hepatopancreas and shrimp individuals were divided into vibrios-resistance (VR) groups and vibrios-susceptible (VS) groups according to their vibrios-resistance performance (VRP). Expression assay of LvLTLC1 showed that mRNA levels of LvLTLC1 in the hepatopancreas of the VR group was significantly higher than that in the VS groups. Totally, 24 SNPs were identified in the promoter regions of LvLTLC1 in shrimp individuals. Correlation analysis between the genotypes of these single nucleotide polymorphism (SNPs) and the VRP revealed that a homozygous GG genotype in SNP8 (g.–256 A/G) were significantly correlated with the VRP (P < 0.05). Linkage disequilibrium (LD) analysis revealed a high linkage block of SNP9 (g.–249 T/G) and SNP10 (g.–235 A/G), and a TT/GG combined genotype of SNP9 and SNP10 was further discovered to be significantly associated with strong VRP (P < 0.05). Furthermore, the variations in motifs caused by SNP8, SNP9, and SNP10 led to changes of binding patterns of transcription factors. The present study first revealed the presence of abundant SNPs in a gene promoter region of shrimp L. vannamei and found that specific genotypes of SNPs in LvLTLC1 promoter confer strong VRP in the shrimp. The homozygous GG genotype in SNP8 and the TT/GG genotype combination of SNP9 and SNP10 can be served as two potential molecular markers for the PVR trait in genetic breeding of L. vannamei.

{"title":"Correlation between SNPs in promoter region of gene, L-Type lectin (LvLTLC1) and the resistance trait of shrimp, Litopenaeus vannamei, against vibrios","authors":"Shuyang Wen , Lihong Yuan , Jiayue Yin , Chaoqun Hu , Xin Zhang , Yunyi Huang , Chujie Zhao , Liyan Liu , Peng Luo","doi":"10.1016/j.aqrep.2025.102725","DOIUrl":"10.1016/j.aqrep.2025.102725","url":null,"abstract":"<div><div>Pacific white shrimp, <em>Litopenaeus vannamei</em> (<em>L. vannamei</em>), is the most economically valuable shrimp species in world. Bacterial diseases mainly caused by various vibrios bring huge economic loss in shrimp aquaculture. Previous research has confirmed that <em>LvLTLC1</em> gene coding a L-type lectin of <em>L. vannamei</em> plays a pivotal role in the resistance of shrimp against pathogenic vibrios, which implies the potential of <em>LvLTLC1</em> as a target gene to develop SNP markers for genetic breeding of the shrimp. In this study, pan-vibrio resistance (PVR) trait of <em>L. vannamei</em> was quantitatively evaluated by vibrios loads in hepatopancreas and shrimp individuals were divided into vibrios-resistance (VR) groups and vibrios-susceptible (VS) groups according to their vibrios-resistance performance (VRP). Expression assay of <em>LvLTLC1</em> showed that mRNA levels of <em>LvLTLC1</em> in the hepatopancreas of the VR group was significantly higher than that in the VS groups. Totally, 24 SNPs were identified in the promoter regions of <em>LvLTLC1</em> in shrimp individuals. Correlation analysis between the genotypes of these single nucleotide polymorphism (SNPs) and the VRP revealed that a homozygous GG genotype in SNP8 (g.–256 A/G) were significantly correlated with the VRP (<em>P</em> < 0.05). Linkage disequilibrium (LD) analysis revealed a high linkage block of SNP9 (g.–249 T/G) and SNP10 (g.–235 A/G), and a TT/GG combined genotype of SNP9 and SNP10 was further discovered to be significantly associated with strong VRP (<em>P</em> < 0.05). Furthermore, the variations in motifs caused by SNP8, SNP9, and SNP10 led to changes of binding patterns of transcription factors. The present study first revealed the presence of abundant SNPs in a gene promoter region of shrimp <em>L. vannamei</em> and found that specific genotypes of SNPs in <em>LvLTLC1</em> promoter confer strong VRP in the shrimp. The homozygous GG genotype in SNP8 and the TT/GG genotype combination of SNP9 and SNP10 can be served as two potential molecular markers for the PVR trait in genetic breeding of <em>L. vannamei.</em></div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"41 ","pages":"Article 102725"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143520147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of immune responses in juvenile Acanthopagrus latus to Aeromonas hydrophila infection revealed by multi-tissue transcriptomic analysis

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2025-03-01 DOI: 10.1016/j.aqrep.2025.102724

Hong-Di Fan , Min Liu , Yuan-Ming Zhao , Xu Wang , Jia Lin , Qing Zhu , Wen Huang , Jiang-Hai Wang

Yellowfin seabream (Acanthopagrus latus) is an economically important species extensively cultivated along the southeast coastal sea of China. This species is frequently afflicted by infections from Aeromonas hydrophila, a pathogen that causes widespread morbidity and mortality. In this study, an infection model was established by administering an intraperitoneal injection of A. hydrophila into yellowfin seabream. Subsequent observations and histopathological assessments revealed considerable damages of multi-tissues after the infection. Biochemical serum assays and measurements of immune-related enzyme activities indicated evident metabolic disturbances and inflammatory pathways in the affected fish. RNA sequencing analysis identified 1615 differentially expressed genes (DEGs) in the liver and 2578 DEGs in the intestine. KEGG mapping revealed significantly regulated pathways, including the C-type lectin receptor signaling pathway and MAPK signaling pathway in the liver, and calcium signaling pathway and cytokine-cytokine receptor interaction in the intestine. A protein-protein interaction network was also constructed for pivotal genes, such as cxcr4b and cd44b, which may play crucial roles in the immune and inflammatory pathways of yellowfin seabream. The transcriptomic results were further corroborated by quantitative real-time PCR tests of some specific genes. These novel data have revealed the immune-related pathways and key regulatory genes activated by A. hydrophila infection and may offer a valuable insight into the immune response mechanisms of yellowfin seabream.

{"title":"Mechanisms of immune responses in juvenile Acanthopagrus latus to Aeromonas hydrophila infection revealed by multi-tissue transcriptomic analysis","authors":"Hong-Di Fan , Min Liu , Yuan-Ming Zhao , Xu Wang , Jia Lin , Qing Zhu , Wen Huang , Jiang-Hai Wang","doi":"10.1016/j.aqrep.2025.102724","DOIUrl":"10.1016/j.aqrep.2025.102724","url":null,"abstract":"<div><div>Yellowfin seabream (<em>Acanthopagrus latus</em>) is an economically important species extensively cultivated along the southeast coastal sea of China. This species is frequently afflicted by infections from <em>Aeromonas hydrophila</em>, a pathogen that causes widespread morbidity and mortality. In this study, an infection model was established by administering an intraperitoneal injection of <em>A. hydrophila</em> into yellowfin seabream. Subsequent observations and histopathological assessments revealed considerable damages of multi-tissues after the infection. Biochemical serum assays and measurements of immune-related enzyme activities indicated evident metabolic disturbances and inflammatory pathways in the affected fish. RNA sequencing analysis identified 1615 differentially expressed genes (DEGs) in the liver and 2578 DEGs in the intestine. KEGG mapping revealed significantly regulated pathways, including the C-type lectin receptor signaling pathway and MAPK signaling pathway in the liver, and calcium signaling pathway and cytokine-cytokine receptor interaction in the intestine. A protein-protein interaction network was also constructed for pivotal genes, such as <em>cxcr4b</em> and <em>cd44b</em>, which may play crucial roles in the immune and inflammatory pathways of yellowfin seabream. The transcriptomic results were further corroborated by quantitative real-time PCR tests of some specific genes. These novel data have revealed the immune-related pathways and key regulatory genes activated by <em>A. hydrophila</em> infection and may offer a valuable insight into the immune response mechanisms of yellowfin seabream.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"41 ","pages":"Article 102724"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143527378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0