Aquaculture Reports最新文献

Transcriptomic responses to transportation stress in the juvenile Chinese sea bass (Lateolabrax maculatus) 中国鲈鱼幼鱼对运输压力的转录组反应

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2024-11-02 DOI: 10.1016/j.aqrep.2024.102467

Yue Su , Sarinpan Kokau , Xiao-Ning Zhang , Yun-Wei Dong

Transportation is a necessary aspect of the aquaculture industry that might induce inevitable stress and compromise product quality. In the present study, the transcriptomic profiles in the brain and liver of Chinese sea bass (Lateolabrax maculatus) were characterized after transportation stress at two different densities (low-density: 20 kg/m³ and high-density: 40 kg/m³). Transcriptome analysis revealed that 485 and 627 differentially expressed genes (DEGs) were determined in the brain and liver from sea bass after transportation stress, respectively. KEGG enrichment analysis demonstrated that the DEGs were significantly enriched in focal adhesion, PI3K-Akt, MAPK signaling pathway, apoptosis, and circadian rhythm under transportation stress. Protein-Protein Interaction (PPI) networks showed that COL1A1, COL1A2, COL5A2, COL10A1, and COL16A1 associated with protein digestion and absorption, together with LUM, SERPINH1, and ADAMTS2 were recognized as hub genes in the brain, while JUN, NFKB1, MAPK14, MAP3K7, HSPA1, HSP90AA1, PIK3CA and PIK3R1 tightly related to MAPK and PI3K-Akt signaling pathway and SMAD3 and PPARGC1A were considered as hub genes in the liver. These results indicated that the alterations in transcripts of sea bass are primarily involved in cell adhesion, signal transduction, immune response, and metabolic regulation in response to transportation stress. Our study provides new perspectives into the molecular mechanism underlying responses to transportation and theoretical support for optimizing transportation in the Chinese sea bass.

运输是水产养殖业的一个必要环节，可能会引起不可避免的应激并影响产品质量。本研究对中国鲈鱼（Lateolabrax maculatus）在两种不同密度（低密度：20 千克/立方米和高密度：40 千克/立方米）的运输应激后大脑和肝脏的转录组特征进行了分析。转录组分析表明，运输应激后，海鲈大脑和肝脏中分别有 485 和 627 个差异表达基因（DEGs）。KEGG富集分析表明，在运输应激下，DEGs在病灶粘附、PI3K-Akt、MAPK信号通路、细胞凋亡和昼夜节律等方面明显富集。蛋白质-蛋白质相互作用（PPI）网络显示，与蛋白质消化和吸收相关的COL1A1、COL1A2、COL5A2、COL10A1和COL16A1以及LUM、SERPINH1和ADAMTS2被认为是大脑中的枢纽基因、而与 MAPK 和 PI3K-Akt 信号通路密切相关的 JUN、NFKB1、MAPK14、MAP3K7、HSPA1、HSP90AA1、PIK3CA 和 PIK3R1 以及 SMAD3 和 PPARGC1A 被认为是肝脏中的枢纽基因。这些结果表明，海鲈转录本的变化主要涉及运输应激时的细胞粘附、信号转导、免疫应答和代谢调节。我们的研究为了解中国鲈鱼对运输反应的分子机制提供了新的视角，并为优化中国鲈鱼的运输提供了理论支持。

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引用次数: 0

Induced spawning of tropical sea cucumber, Stichopus fusiformiossa by neurotransmitters: Challenges and way forward 神经递质诱导热带海参产卵：挑战与前进之路

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2024-11-02 DOI: 10.1016/j.aqrep.2024.102464

Kah-Mei Cheong , Izzat Irfan , Mathan Ganasan , Musfirah Zulkurnain , Sau Pinn Woo , Annette Jaya-Ram

It is imperative to develop aquaculture methods for the tropical sea cucumber, Stichopus fusiformiossa as it is an essential component for the pharmaceutical and traditional medicine industry in Malaysia. With sea cucumber stocks harvested freely, conservation efforts should be in place to overcome future depletion or loss of populations in the wild. This study represents the first report to induce spawning of S. fusiformiossa using conventional methods (thermal shock, desiccation, microalgae stimulation) and neurotransmitter injections (serotonin, 1-methyladenine, epinephrine, norepinephrine) in an indoor based aquaculture system. Post induction, the sea cucumbers were observed for 8 hours. They displayed classic sea cucumber pre-spawning behaviours, namely, raising their head, swaying the anterior (side to side, back and forth, head spinning/circling) and prominent visibility of the gonopore. All neurotransmitters used successfully triggered the pre-spawning behaviours, with the group induced with 1-methyladenine remaining active throughout the observation period. During the first 10 minutes (0 hr) of neurotransmitter injection, 1-methyladenine induced group had more than 50 % of the animals with visible gonopores. Generally, body-raising behaviour was observed across all treatments at 0 hr. On the other hand, swaying behaviour was displayed more frequently in all neurotransmitter induced groups, although it remained below 40 % in each case. No swaying action was revealed in the group with raised temperature and microalgae stimulation treated group. While all treatments showed varying degrees of promising pre-spawning behaviour, none spawned during the observation duration. Histology of male and female gonad development stages were also examined randomly. This study describes challenges faced during spawning induction and general maintenance of stichopodid sea cucumbers which potentially undergo autolysis or melting of their external body wall due to physical stress. Utilization of neurotransmitters was unsuccessful; however, the findings from this study paves way for further avenues of research to elucidate the spawning induction techniques of S. fusiformiossa.

开发热带海参（Stichopus fusiformiossa）的水产养殖方法势在必行，因为它是马来西亚制药和传统医药行业的重要组成部分。随着海参种群的自由采捕，应开展保护工作，以克服未来野生种群的枯竭或损失。本研究是首次报道在室内水产养殖系统中使用传统方法（热冲击、干燥、微藻刺激）和神经递质注射（5-羟色胺、1-甲基腺嘌呤、肾上腺素、去甲肾上腺素）诱导 S. fusiformiossa 产卵。诱导后，对海参进行了 8 小时的观察。它们表现出典型的海参产卵前行为，即抬头、前部摇摆（左右摇摆、前后摇摆、头部旋转/盘旋）以及生殖孔明显可见。所有使用的神经递质都成功地触发了产卵前行为，其中使用1-甲基腺嘌呤诱导的组在整个观察期间保持活跃。在注射神经递质的前 10 分钟（0 小时），1-甲基腺嘌呤诱导组有 50% 以上的动物可见生殖孔。一般来说，在 0 小时内，所有处理都能观察到身体上扬的行为。另一方面，所有神经递质诱导组都更频繁地出现摇摆行为，但每组的摇摆率都低于 40%。温度升高组和微藻类刺激处理组则没有摇摆行为。虽然所有处理组在产卵前都表现出不同程度的产卵行为，但在观察期间均未产卵。此外，还对雌雄性腺发育阶段的组织学进行了随机检查。本研究描述了毛足类海参在产卵诱导和一般维护过程中面临的挑战，由于物理压力，毛足类海参的外体壁可能会发生自溶或融化。神经递质的使用并不成功；不过，本研究的发现为进一步研究阐明栉水母的产卵诱导技术铺平了道路。

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引用次数: 0

Possible involvement of Jun, Foxo1, Nobox in the regulation of smad2/3 transcription in the protogynous hermaphroditic ricefield eel (Monopterus albus) Jun、Foxo1和Nobox可能参与调控原雌雄同体稻田鳗（Monopterus albus）的smad2/3转录

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2024-11-02 DOI: 10.1016/j.aqrep.2024.102465

Zhi He , Haochen Wang , Li Zheng , Qiqi Chen, Jinxin Xiong, Mingqiang Chen, Wenxiang Ding, Junjie Huang, Kuo Gao, Bolin Lai, Zhijun Ma, Ziting Tang, Mingwang Zhang, Deying Yang, Taiming Yan

Small mothers against decapentaplegic2/3 (Smad2/3) are two signalling intermediates in the transforming growth factor beta (TGF-β) signalling pathway, which plays a critical role in gonad development. However, a systemic analysis of Smad2/3 in Monopterus albus (M. albus) has not been performed. The smad2 sequence in M. albus was characterized in our previous publication. In this study, we characterized the nucleotide sequence of smad3 and reported that smad3 is widely expressed in different tissues of M. albus. Importantly, the expression of smad3 significantly increased at the early vitellogenic stage in the ovary and decreased with oocyte maturation. Next, we aimed to identify the upstream factors that regulate Smad2/3 transcription. The 5′ flanking sequences of smad2/3 were cloned, and their core promoter regions were determined to be between −447 bp and −100 bp and between −1520 bp and −927 bp, respectively. The Jun and Foxo1 binding sites were conserved in the smad2 promoter region, and the Sp1, Bcl-6, and Nobox binding sites were conserved in the smad3 promoter region. Through dual-luciferase reporter gene experiments, we showed that Jun and Foxo1 binding sites may be involved in the regulation of smad2 transcription, whereas the Nobox binding site may be involved in the activation of the smad3 promoter.

抗癸型截瘫小母细胞2/3（Smad2/3）是转化生长因子β（TGF-β）信号通路中的两个信号中间体，在性腺发育中起着至关重要的作用。然而，尚未对白蝶猴（Monopterus albus）的 Smad2/3 进行系统分析。我们之前发表的文章对白鲑鱼的 smad2 序列进行了表征。在本研究中，我们对 smad3 的核苷酸序列进行了表征，并报告了 smad3 在白喉猴不同组织中的广泛表达。重要的是，smad3的表达在卵巢早期卵黄形成阶段显著增加，并随着卵母细胞的成熟而减少。接下来，我们旨在确定调控 Smad2/3 转录的上游因子。克隆了Smad2/3的5′侧翼序列，确定其核心启动子区域分别在-447 bp至-100 bp和-1520 bp至-927 bp之间。在smad2启动子区域，Jun和Foxo1结合位点是保守的；在smad3启动子区域，Sp1、Bcl-6和Noxbox结合位点是保守的。通过双荧光素酶报告基因实验，我们发现 Jun 和 Foxo1 结合位点可能参与了 smad2 的转录调控，而 Nobox 结合位点可能参与了 smad3 启动子的激活。

{"title":"Possible involvement of Jun, Foxo1, Nobox in the regulation of smad2/3 transcription in the protogynous hermaphroditic ricefield eel (Monopterus albus)","authors":"Zhi He , Haochen Wang , Li Zheng , Qiqi Chen, Jinxin Xiong, Mingqiang Chen, Wenxiang Ding, Junjie Huang, Kuo Gao, Bolin Lai, Zhijun Ma, Ziting Tang, Mingwang Zhang, Deying Yang, Taiming Yan","doi":"10.1016/j.aqrep.2024.102465","DOIUrl":"10.1016/j.aqrep.2024.102465","url":null,"abstract":"<div><div>Small mothers against decapentaplegic2/3 (Smad2/3) are two signalling intermediates in the transforming growth factor beta (TGF-β) signalling pathway, which plays a critical role in gonad development. However, a systemic analysis of Smad2/3 in <em>Monopterus albus</em> (<em>M. albus</em>) has not been performed. The <em>smad2</em> sequence in <em>M. albus</em> was characterized in our previous publication. In this study, we characterized the nucleotide sequence of <em>smad3</em> and reported that <em>smad3</em> is widely expressed in different tissues of <em>M. albus</em>. Importantly, the expression of <em>smad3</em> significantly increased at the early vitellogenic stage in the ovary and decreased with oocyte maturation. Next, we aimed to identify the upstream factors that regulate Smad2/3 transcription. The 5′ flanking sequences of <em>smad2/3</em> were cloned, and their core promoter regions were determined to be between −447 bp and −100 bp and between −1520 bp and −927 bp, respectively. The Jun and Foxo1 binding sites were conserved in the <em>smad2</em> promoter region, and the Sp1, Bcl-6, and Nobox binding sites were conserved in the <em>smad3</em> promoter region. Through dual-luciferase reporter gene experiments, we showed that Jun and Foxo1 binding sites may be involved in the regulation of <em>smad2</em> transcription, whereas the Nobox binding site may be involved in the activation of the <em>smad3</em> promoter.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102465"},"PeriodicalIF":3.2,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142578214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early mucosal responses in common carp (Cyprinus carpio) to the infection of Aeromonas hydrophila 鲤鱼对嗜水气单胞菌感染的早期粘膜反应

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2024-10-31 DOI: 10.1016/j.aqrep.2024.102466

Jiali Wang , Qi Zhou , Hongyu Zhang , Yanliang Jiang

Common carp (Cyprinus carpio), an economically important freshwater fish species, is worldwide farmed especially in Europe and Asia. The current high-density intensive rearing way leading to a high susceptibility to various pathogens, and Aeromonas hydrophila is one of the most frequently encountered bacteria, causing huge economic losses to the common carp industry. The mucosal barrier of fish constitutes the first line of defense against various pathogens, and some level of inter-connectivity exists among teleost mucosal tissues. However, the molecular basis for a common mucosal immune response at multiple sites or response at one site but during different timepoints following stimulation remains to be studied. In this study, we examined and systematically analyzed a total of 144 transcriptome files from three primary common carp mucosal tissues at four early timepoints following the infection of A. hydrophila. A total of 410 unigenes were significantly differentially expressed in all tested mucosal tissues. Through GO enrichment and KEGG pathway analysis, 28 key genes which might played critical roles in the common mucosal immune response of common carp during the early stage of bacterial infection were identified. Further WGCNA analysis showed that, besides the common response, three mucosal tissues exhibited tissue-specific gene regulatory network. Meanwhile, candidate hub genes in each tissue were identified, including trim3, rpl14, tln2, itpr1, and others. Our results will provide a fundamental basis for understanding the molecular mechanisms of teleost mucosal immunity, and might suggest strategies for developing novel teleost mucosal vaccines in aquaculture.

鲤鱼（Cyprinus carpio）是一种具有重要经济价值的淡水鱼种，全世界都有养殖，尤其是在欧洲和亚洲。目前的高密度集约化饲养方式导致了对各种病原体的高度敏感性，而嗜水气单胞菌（Aeromonas hydrophila）是最常遇到的细菌之一，给鲤鱼业造成了巨大的经济损失。鱼类的粘膜屏障是抵御各种病原体的第一道防线，而且远东鱼类粘膜组织之间存在一定程度的相互联系。然而，在多个部位产生共同的粘膜免疫反应，或在一个部位产生反应但在刺激后的不同时间点产生反应的分子基础仍有待研究。在本研究中，我们研究并系统分析了三种主要鲤鱼粘膜组织在感染纤毛虫后四个早期时间点的共 144 个转录组文件。在所有检测的粘膜组织中，共有 410 个单体基因有显著的差异表达。通过 GO 富集和 KEGG 通路分析，确定了 28 个关键基因，这些基因可能在细菌感染早期鲤鱼粘膜免疫反应中发挥关键作用。进一步的 WGCNA 分析表明，除了常见的反应外，三个粘膜组织还表现出组织特异性基因调控网络。同时，还发现了各组织中的候选枢纽基因，包括 trim3、rpl14、tln2、itpr1 等。我们的研究结果将为了解远东鱼类粘膜免疫的分子机制提供基础，并为开发新型远东鱼类粘膜疫苗提供策略建议。

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引用次数: 0

Effects of dietary of bile acid on growth performance, antioxidant capacity, liver and intestinal health and immune function in loach (Misgurnus anguillicaudatus) 胆汁酸对泥鳅生长性能、抗氧化能力、肝脏和肠道健康以及免疫功能的影响

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2024-10-31 DOI: 10.1016/j.aqrep.2024.102455

Haihui Liu , Xinyu Zhang , Ke Li , Qiongya Fang , Xiaorui Liu , Li Sun , Guiliang Guo , Siyu An , Min Li , Guiqin Wang , Yidi Kong

Bile acid (BA) play an important role in nutrient absorption and are key regulators of antioxidant and immune response homeostasis. The purpose of this study was to evaluate the effects of dietary supplementation with 0 (CON), 100 (BA100), 200 (BA200), 300 (BA300) and 400 (BA400) mg/kg BA on the growth, digestive enzyme activity, antioxidant capacity and immune response of loach. The results of serum biochemical analysis showed that the serum markers of liver damage (ALT, AST, ALP and LDH) of loach were decreased by adding BA. The BA diets mainly affected the lipase activity in the liver and intestine of loach, but there was no significant difference on protease. Histological section results showed that dietary supplementation with 100, 200 and 300 mg/kg BA increased the number of liver nuclei, liver vacuolation, intestinal wall muscle thickness and intestinal villi. In addition, BA enhanced the antioxidant capacity of loach by increasing the activities of GSH, GSH-Px, CAT, SOD and GST in their tissues (liver and intestine) and the mRNA expression levels of antioxidant-related genes (cat, gsh-px, sod and nrf2). At the same time, BA increased serum immune parameters (C3, C4, IgM and LYS), up-regulated the mRNA expression levels of anti-inflammatory factors (il-10), key immune genes (lys) and down-regulated the mRNA expression levels of pro-inflammatory factors (il-1β and tnf-α) in tissues (liver and intestine). In conclusion, dietary BA supplementation could improve the liver and intestinal digestive enzyme activities, enhance the antioxidant capacity and immune function, and promote the healthy growth of loach. Based on the results of these studies, the optimal supplementation of BA in loach diets was 200–300 mg/kg.

胆汁酸（BA）在营养吸收中起着重要作用，是抗氧化和免疫反应平衡的关键调节剂。本研究的目的是评估膳食补充 0（CON）、100（BA100）、200（BA200）、300（BA300）和 400（BA400） mg/kg BA 对泥鳅生长、消化酶活性、抗氧化能力和免疫反应的影响。血清生化分析结果表明，添加 BA 后，泥鳅血清中的肝损伤指标（ALT、AST、ALP 和 LDH）均有所下降。BA日粮主要影响泥鳅肝脏和肠道中脂肪酶的活性，但对蛋白酶的影响无显著差异。组织学切片结果显示，日粮中添加 100、200 和 300 mg/kg 的 BA 会增加肝核数量、肝空泡化、肠壁肌肉厚度和肠绒毛。此外，BA 还能提高泥鳅组织（肝脏和肠道）中 GSH、GSH-Px、CAT、SOD 和 GST 的活性以及抗氧化相关基因（cat、gsh-px、sod 和 nrf2）的 mRNA 表达水平，从而增强泥鳅的抗氧化能力。同时，BA 能提高血清免疫参数（C3、C4、IgM 和 LYS），上调抗炎因子（il-10）和关键免疫基因（lys）的 mRNA 表达水平，下调组织（肝脏和肠道）中促炎因子（il-1β 和 tnf-α）的 mRNA 表达水平。总之，饵料中添加 BA 可提高泥鳅肝脏和肠道消化酶活性，增强抗氧化能力和免疫功能，促进泥鳅健康生长。根据这些研究结果，泥鳅日粮中最佳的 BA 添加量为 200-300 mg/kg。

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引用次数: 0

Mechanisms of Amomum villosum essential oil in enhancing tilapia (Oreochromis niloticus) intestinal health Amomum villosum 精油增强罗非鱼（Oreochromis niloticus）肠道健康的机制

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2024-10-31 DOI: 10.1016/j.aqrep.2024.102451

Xiangbing Zeng , Xiaoting Zheng , Chenghui Li , Junchao Ming , Hongbiao Dong , Jiasong Zhang

Amomum villosum is a member of the ginger family to treat gastrointestinal diseases in humans. Supplementing the diet of tilapia (Oreochromis niloticus) with essential oil (EOA) can enhance digestive and intestinal antioxidant capacity. We conducted a comparative experiment using tilapia to further understand the potential molecular response mechanism to dietary EOA. Fish were divided into four groups with different feeding doses of EOA: L (2 g/Kg) group, M (4 g/Kg) group, and H (8 g/Kg) group, along with a control group without EOA supplementation. The level of inflammatory factors (TNF-α, IL-1) declined significantly in the EOA group. Comparative transcriptome of the intestines revealed differentially expressed genes (DEGs) in each group, which further revealed that enriched terms were closely related to important pathways such as PI3K-Akt signaling, cell adhesion molecules, regulation of actin cytoskeleton, and autoimmune thyroid disease. These pathways play significant roles in regulating various cellular processes and immune responses of intestines. Intestine metabolomic analysis indicated that dietary EOA caused changes in metabolic processes, particularly in nucleotide metabolism, indicating the potential impact of EOA on these processes. To identify key factors regulating the molecular response to EOA supplementation, a weighted gene co-expression network analysis combined with transcriptome and differential metabolites to identify key genes and metabolites. Glycitein and genistein are substances related to the metabolism of soybean meal were identified. Hmgb2, Parp11, Bcoll is considered to be the key genes, and has the immune regulation function. This study provides new insights into the molecular basis underlying the protective effects of dietary EOA in tilapia. The results can benefit the aquaculture industry by developing dietary strategies to improve the health and welfare of tilapia in intensive farming.

Amomum villosum 是姜科植物，可治疗人类的胃肠道疾病。在罗非鱼（Oreochromis niloticus）膳食中添加精油（EOA）可提高消化系统和肠道的抗氧化能力。我们利用罗非鱼进行了一项比较实验，以进一步了解膳食 EOA 的潜在分子反应机制。我们将罗非鱼分为四组，分别投喂不同剂量的 EOA：L组（2 g/Kg）、M组（4 g/Kg）和H组（8 g/Kg），以及未添加EOA的对照组。EOA组的炎症因子（TNF-α、IL-1）水平明显下降。肠道转录组比较显示了各组的差异表达基因（DEGs），进一步发现富集的术语与重要通路密切相关，如 PI3K-Akt 信号、细胞粘附分子、肌动蛋白细胞骨架调节和自身免疫性甲状腺疾病。这些通路在调节肠道的各种细胞过程和免疫反应中发挥着重要作用。肠道代谢组学分析表明，膳食中的环氧乙烷会引起代谢过程的变化，尤其是核苷酸代谢的变化，这表明环氧乙烷对这些过程有潜在的影响。为了确定调节补充 EOA 分子反应的关键因素，加权基因共表达网络分析结合转录组和差异代谢物确定了关键基因和代谢物。鉴定出了与豆粕代谢有关的物质--亚甘氨酸和染料木素。Hmgb2、Parp11、Bcoll被认为是关键基因，具有免疫调节功能。本研究为了解膳食 EOA 对罗非鱼保护作用的分子基础提供了新的视角。研究结果可为水产养殖业带来益处，有助于制定日粮策略，改善集约化养殖罗非鱼的健康和福利。

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引用次数: 0

Impact of increasing temperatures on neuroendocrine and molecular responses of skeletal muscle and liver in fish: A comprehensive review 温度升高对鱼类骨骼肌和肝脏的神经内分泌和分子反应的影响：综述

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2024-10-31 DOI: 10.1016/j.aqrep.2024.102448

Alberto Sáez-Arteaga , Ivan Viegas , Mariana Palma , Patricio Dantagnan , Iván Valdebenito , Elías Figueroa Villalobos , Adrián Hernández , Jimena Guerrero-Jiménez , Isidoro Metón , Cristopher Heyser

Recent projections suggest that food security may face threats due to climate change and population growth. Global warming is altering ocean temperatures, impacting productivity and aquatic ecosystems. This review delves into the impact of heat stress on fish aquaculture, focusing on the neuroendocrine system, controlled by the hypothalamic-pituitary-interrenal (HPI) axis. This axis regulates stress responses via cortisol and catecholamines, influencing energy metabolism and stress adaptation. The review discusses molecular adaptations in skeletal muscle and liver, affecting gene expression related to growth and energy metabolism. On the other hand, heat stress provokes notable molecular responses, altering the expression of genes involved in glycolysis and oxidative phosphorylation, and hormonal pathways like growth hormone and insulin growth factors, which are vital for metabolic regulation and muscle growth. In summary, this review provides insights into the neuroendocrine and molecular mechanisms underlying fish responses to thermal stress, emphasizing the importance of skeletal muscle and liver tissues in mediating physiological adaptations. Furthermore, it analyzes the implications for protein synthesis, cellular signaling pathways, oxidative stress, and enzymatic responses in aquatic organisms, highlighting the importance of understanding these molecular adaptations to develop effective mitigation strategies that ensure the sustainability of aquaculture systems amidst changing conditions.

最近的预测表明，由于气候变化和人口增长，粮食安全可能面临威胁。全球变暖正在改变海洋温度，影响生产力和水生生态系统。本综述深入探讨了热应激对水产养殖的影响，重点是由下丘脑-垂体-肾上腺（HPI）轴控制的神经内分泌系统。该轴通过皮质醇和儿茶酚胺调节应激反应，影响能量代谢和应激适应。综述讨论了骨骼肌和肝脏的分子适应性，影响与生长和能量代谢有关的基因表达。另一方面，热应激会引起显著的分子反应，改变参与糖酵解和氧化磷酸化的基因表达，以及生长激素和胰岛素生长因子等激素途径，这些对新陈代谢调节和肌肉生长至关重要。总之，本综述深入探讨了鱼类对热应激反应的神经内分泌和分子机制，强调了骨骼肌和肝脏组织在介导生理适应方面的重要性。此外，它还分析了水生生物蛋白质合成、细胞信号通路、氧化应激和酶反应的影响，强调了了解这些分子适应性对制定有效缓解策略的重要性，以确保水产养殖系统在不断变化的条件下的可持续性。

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引用次数: 0

Inclusion of different blends of poultry processed proteins in practical diets for African catfish (Clarias gariepinus) reared in RAS: Effects on nutrient digestibility, growth performance and fillet quality 在 RAS 饲养的非洲鲶鱼（Clarias gariepinus）的实用日粮中添加不同的家禽加工蛋白混合物：对营养物质消化率、生长性能和鱼片质量的影响

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2024-10-30 DOI: 10.1016/j.aqrep.2024.102447

Cecilia Fanizza , Angela Trocino , Vlastimil Stejskal , Markéta Prokešová , Mahyar Zare , Hung Quang Tran , Fabio Brambilla , Gerolamo Xiccato , Francesco Bordignon

The present study aimed to test the effects of different dietary inclusion levels of fishmeal (F) and blends of poultry processed protein meals (P), i.e. poultry by-product meal, hydrolysed feather meal, dry-blood meal in African catfish (Clarias gariepinus). Fish were fed during 84 days with four practical diets, i.e. diet HF-LP (F: 90 g kg⁻¹; P: 227 g kg⁻¹), diet MF-MP (F: 45 g kg⁻¹; P: 280 g kg⁻¹), diet HF-HP (F: 90 g kg⁻¹; P: 315 g kg⁻¹), and diet LF-HP (F: 10 g kg⁻¹; P: 392 g kg⁻¹). Fish fed diet LF-HP showed the lowest protein and lipid digestibility coefficients (82 % and 92 % vs. 84 % and 94 %, respectively; p<0.05) compared to fish fed the other diets, and the worst performance in terms of final body weight (551 g vs. 673 g), feed intake (247 g d⁻¹ vs. 301 g d⁻¹), condition factor (0.83 vs. 0.86), specific growth rate (2.69 % d⁻¹ vs. 3.14 % d⁻¹), and feed conversion ratio (1.34 vs. 1.11) (p<0.001). Fish fed diet MF-MP reported the highest survival rate compared to other diets (96.8 % vs. 94.5 %; p<0.001). Fish fed diets with the highest inclusion rates of poultry by-product meal (140 g kg⁻¹ or 165 g kg⁻¹) and hydrolysed feather meal (125 g kg⁻¹ or 135 g kg⁻¹) were associated with the lowest fillet yield (46.2 % vs. 47.1 %) respect to fish fed the other diets (p<0.05). In conclusion, a diet with 10 g kg⁻¹ of fishmeal and 392 g kg⁻¹ of a blend of poultry processed animal proteins is not suitable to sustain the performance of African catfish, whereas a diet with a minimum content of fishmeal at 45 g kg⁻¹ and a blend of poultry processed animal proteins at about 280 g kg⁻¹ could be technically and economically attractive for the RAS farming of this species.

本研究旨在测试非洲鲶鱼（Clarias gariepinus）不同日粮中鱼粉（F）和家禽加工蛋白粉（P）（即家禽副产品粉、水解羽毛粉、干血粉）混合物含量的影响。用四种实用日粮喂养非洲鲶鱼 84 天，即 HF-LP 日粮（F：90 克/千克-1；P：227 克/千克-1）、MF-MP 日粮（F：45 克/千克-1；P：280 克/千克-1）、HF-HP 日粮（F：90 克/千克-1；P：315 克/千克-1）和 LF-HP 日粮（F：10 克/千克-1；P：392 克/千克-1）。与饲喂其他日粮的鱼类相比，饲喂 LF-HP 日粮的鱼类蛋白质和脂质消化系数最低（分别为 82 % 和 92 % vs. 84 % 和 94 %；p<0.05），最终体重（551 克 vs. 673 克）、饲料摄入量（10 kg-1; P: 392 kg-1）和饲料消耗量（10 kg-1; P: 392 kg-1）表现最差。673 g）、摄食量（247 g d-1 vs. 301 g d-1）、体况系数（0.83 vs. 0.86）、特定生长率（2.69 % d-1 vs. 3.14 % d-1）和饲料转化率（1.34 vs. 1.11）方面的表现最差（p<0.001）。与其他日粮相比，饲喂 MF-MP 日粮的鱼类存活率最高（96.8 % 对 94.5 %；p<0.001）。与喂食其他日粮的鱼类相比，喂食添加率最高的家禽副产品粉（140 克/千克-1 或 165 克/千克-1）和水解羽毛粉（125 克/千克-1 或 135 克/千克-1）的鱼类的鱼片产量最低（46.2% 对 47.1%）（p<0.05）。总之，10 克/公斤-1 的鱼粉和 392 克/公斤-1 的家禽加工动物蛋白混合日粮不适合维持非洲鲶鱼的生长性能，而最低含量为 45 克/公斤-1 的鱼粉和大约 280 克/公斤-1 的家禽加工动物蛋白混合日粮在技术和经济上对非洲鲶鱼的 RAS 养殖都有吸引力。

{"title":"Inclusion of different blends of poultry processed proteins in practical diets for African catfish (Clarias gariepinus) reared in RAS: Effects on nutrient digestibility, growth performance and fillet quality","authors":"Cecilia Fanizza , Angela Trocino , Vlastimil Stejskal , Markéta Prokešová , Mahyar Zare , Hung Quang Tran , Fabio Brambilla , Gerolamo Xiccato , Francesco Bordignon","doi":"10.1016/j.aqrep.2024.102447","DOIUrl":"10.1016/j.aqrep.2024.102447","url":null,"abstract":"<div><div>The present study aimed to test the effects of different dietary inclusion levels of fishmeal (F) and blends of poultry processed protein meals (P), i.e. poultry by-product meal, hydrolysed feather meal, dry-blood meal in African catfish (<em>Clarias gariepinus</em>). Fish were fed during 84 days with four practical diets, i.e. diet HF-LP (F: 90 g kg<sup>−1</sup>; P: 227 g kg<sup>−1</sup>), diet MF-MP (F: 45 g kg<sup>−1</sup>; P: 280 g kg<sup>−1</sup>), diet HF-HP (F: 90 g kg<sup>−1</sup>; P: 315 g kg<sup>−1</sup>), and diet LF-HP (F: 10 g kg<sup>−1</sup>; P: 392 g kg<sup>−1</sup>). Fish fed diet LF-HP showed the lowest protein and lipid digestibility coefficients (82 % and 92 % vs. 84 % and 94 %, respectively; <em>p</em><0.05) compared to fish fed the other diets, and the worst performance in terms of final body weight (551 g vs. 673 g), feed intake (247 g d<sup>−1</sup> vs. 301 g d<sup>−1</sup>), condition factor (0.83 vs. 0.86), specific growth rate (2.69 % d<sup>−1</sup> vs. 3.14 % d<sup>−1</sup>), and feed conversion ratio (1.34 vs. 1.11) (<em>p</em><0.001). Fish fed diet MF-MP reported the highest survival rate compared to other diets (96.8 % vs. 94.5 %; <em>p</em><0.001). Fish fed diets with the highest inclusion rates of poultry by-product meal (140 g kg<sup>−1</sup> or 165 g kg<sup>−1</sup>) and hydrolysed feather meal (125 g kg<sup>−1</sup> or 135 g kg<sup>−1</sup>) were associated with the lowest fillet yield (46.2 % vs. 47.1 %) respect to fish fed the other diets (<em>p</em><0.05). In conclusion, a diet with 10 g kg<sup>−1</sup> of fishmeal and 392 g kg<sup>−1</sup> of a blend of poultry processed animal proteins is not suitable to sustain the performance of African catfish, whereas a diet with a minimum content of fishmeal at 45 g kg<sup>−1</sup> and a blend of poultry processed animal proteins at about 280 g kg<sup>−1</sup> could be technically and economically attractive for the RAS farming of this species.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102447"},"PeriodicalIF":3.2,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142538369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization and innate immune defense functional analysis of tartrate-resistant acid phosphatase 5 in Trachinotus ovatus 卵棘鲷抗酒石酸磷酸酶 5 的分子特征和先天免疫防御功能分析

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2024-10-30 DOI: 10.1016/j.aqrep.2024.102439

Reng Qiu , Jinge Wang , Qiaoli Yang , Ying Wu , Chunsheng Liu , Yang Lei , Yun Sun

The enzyme tartrate-resistant acid phosphatase 5 (TRAP5) performs an important biological function in immune defense. In the present study, the TRAP5 of Trachinotus ovatus (golden pompano, TroTRAP5) was cloned to assess its biological function. The open reading frame of TroTRAP5 was 990 bp in length and its mature peptide encoded a 34.775-kDa protein that contained a metallophosphatase superfamily domain. Homology sequence alignment revealed that the similarity among teleost fish was 57.66 %–75.99 %. Meanwhile, phylogenetic tree analysis showed that TroTRAP5 was clustered into one branch with teleost TRAP5. TroTRAP5 expression was the most abundant in immune organs (liver, spleen, head kidney, and gills) and up-regulated during bacterial infection. Of note, purified recombinant TroTRAP5 (rTroTRAP5) exhibited phosphatase activity, and the optimal pH and temperature for its activity were 6.0 and 50 ℃. In vivo experiments indicated that TroTRAP5 overexpression in T. ovatus significantly reduced bacterial infection in fish tissues, whereas TroTRAP5 knockdown increased bacterial dissemination and colonization in fish tissues. In summary, the present study results suggest that TroTRAP5 participates in the antibacterial immune response.

抗酒石酸磷酸酶 5（TRAP5）在免疫防御中发挥着重要的生物学功能。本研究克隆了金鲳的 TRAP5，以评估其生物学功能。TroTRAP5的开放阅读框长度为990 bp，其成熟肽编码一个34.775-kDa的蛋白，含有金属磷酸酶超家族结构域。同源序列比对结果表明，TroTRAP5在远缘鱼类中的相似度为57.66%-75.99%。同时，系统发生树分析表明，TroTRAP5与远缘鱼类的TRAP5聚为一个分支。TroTRAP5在免疫器官（肝脏、脾脏、头肾和鳃）中的表达量最高，并在细菌感染时上调。值得注意的是，纯化的重组 TroTRAP5（rTroTRAP5）具有磷酸酶活性，其活性的最佳 pH 值和温度分别为 6.0 和 50 ℃。体内实验表明，TroTRAP5 在卵形栉水母中的过表达能显著减少细菌在鱼体组织中的感染，而 TroTRAP5 的敲除则会增加细菌在鱼体组织中的传播和定殖。总之，本研究结果表明，TroTRAP5 参与了抗菌免疫反应。

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引用次数: 0

Effects of dietary creatine on the muscle growth, flesh quality, lipid metabolism and antioxidant status in juvenile grass carp (Ctenopharyngodon idellus) 膳食肌酸对草鱼幼鱼肌肉生长、肉质、脂质代谢和抗氧化状态的影响

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2024-10-30 DOI: 10.1016/j.aqrep.2024.102449

Yu-Long Gong , Xiao-Hong Lai , Yao Xiao , Peng Jiang , Yu-Feng Song

To elevate the effects of creatine on muscle growth, flesh quality, lipid metabolism and antioxidant responses of aquatic animals, we set up three diets with dietary creatine levels at 0 % (Control), 1 % and 2 % for juvenile grass carp (4.33 ± 0.02 g). Experimental fish were stocked in nine 300-L tanks (30 fish/tank) and randomly assigned with one of the three diets for 8 weeks. We found that 2 % dietary creatine improved the growth performance (SGR) and feed utilization (FCR) of the experimental grass carp. Dietary creatine supplementation further increased the diameter of muscle fibers through elevating the expression levels of muscle growth-related genes (pax3, mrf4, myog, myf5, mef2a, and mef2d) and proteins (Pax3, Myog and Mef2a). Furthermore, both 1 % and 2 % creatine supplementation elevated the muscle flesh quality of grass carp, indicated by the enhanced water holding capacity, improved texture properties, increased flavor compounds (ﬂavor nucleotides and amino acids) content and decreased odorants compounds (2-MIB and GSM) content in the muscle. Dietary creatine supplementation also reduced muscle lipid deposition main forced by suppressing lipogenesis and activating fatty acids β-oxidation. Moreover, the increased antioxidant capability and reduced oxidative stress were found in the muscle of creatine supplementation groups, as evaluated by Nrf2 nuclear-translocation, enzymatic activities, antioxidative genes and proteins expression. Therefore, our study suggested that 1 % or 2 % dietary creatine levels not only promoted the muscle growth, but also enhanced the flesh quality of juvenile grass carp.

为了提高肌酸对水生动物肌肉生长、肉质、脂质代谢和抗氧化反应的影响，我们为草鱼幼鱼（4.33 ± 0.02 g）设置了三种日粮，日粮肌酸水平分别为 0 %（对照组）、1 % 和 2 %。实验鱼被放养在 9 个 300 升的水箱中（每箱 30 尾鱼），并随机分配到三种日粮中的一种，持续 8 周。我们发现，2% 的膳食肌酸提高了实验草鱼的生长性能（SGR）和饲料利用率（FCR）。通过提高肌肉生长相关基因（pax3、mrf4、myog、myf5、mef2a和mef2d）和蛋白质（Pax3、Myog和Mef2a）的表达水平，膳食肌酸补充剂进一步增加了肌肉纤维的直径。此外，补充 1 % 和 2 % 肌酸都能提高草鱼肌肉的肉质，表现在持水力增强、质地改善、风味化合物（风味核苷酸和氨基酸）含量增加以及异味化合物（2-MIB 和 GSM）含量减少。膳食中补充肌酸还能通过抑制脂肪生成和激活脂肪酸的β-氧化作用，减少肌肉脂质沉积。此外，通过 Nrf2 核转移、酶活性、抗氧化基因和蛋白质的表达评估，发现肌酸补充组肌肉的抗氧化能力增强，氧化应激减少。因此，我们的研究表明，1 % 或 2 % 的膳食肌酸水平不仅能促进草鱼幼鱼的肌肉生长，还能提高其肉质。

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引用次数: 0