Detection of Aristolochic Acid Using a Piezoelectric Immunosensor Based on Magnetic Carbon Nanocomposites

IF 0.9 4区材料科学 Q4 MATERIALS SCIENCE, MULTIDISCIPLINARY Inorganic Materials Pub Date : 2024-07-19 DOI:10.1134/s0020168524700092

E. V. Bizina, A. A. Polosina, O. V. Farafonova, S. A. Eremin, T. N. Ermolaeva

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Abstract

This article describes a method for detection of aristolochic acid (AA) in food products using a piezoelectric immunosensor, the recognition layer of which was magnetic carbon nanocomposites (MCNCs) with AA protein conjugates immobilized on their surface. Methods for the synthesis of magnetic Fe₃O₄ nuclei and their fixation on the surface of multiwalled carbon nanotubes (CNTs) have been studied. Using IR spectroscopy, it was established that the formation of the recognition layer of the sensor occurs owing to the formation of covalent bonds between the amino groups of AA conjugates and the carboxyl groups of CNTs. The concentrations of protein conjugates based on ovalbumin (OVA) and bovine serum albumin (BSA) (0.3 and 0.2 mg/mL) and the degree of dilution of antibodies (0.25) providing optimal characteristics of the piezoelectric immunosensor were determined. The metrological characteristics of the determination of AA using the proposed method have been established: the range of determined contents and the detection limit of AA when using a piezoelectric immunosensor with a recognition layer based on MCNC/AA-OVA and MCNC/AA-BSA are 50–400 and 10 ng/mL and 100–300 and 50 ng/mL, respectively. The sensor was tested upon determination of AA in samples of Chinese herbal tea and dietary supplements for weight loss. No acid was found in the tea, but in the dietary supplements, its content is 3.2 μg/g.

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利用基于磁性碳纳米复合材料的压电免疫传感器检测马兜铃酸

摘要本文介绍了一种利用压电免疫传感器检测食品中马兜铃酸（AA）的方法，该传感器的识别层是磁性碳纳米复合材料（MCNCs），其表面固定有 AA 蛋白共轭物。研究了磁性 Fe3O4 核的合成方法以及将其固定在多壁碳纳米管（CNT）表面的方法。利用红外光谱法确定了传感器识别层的形成是由于 AA 共轭物的氨基与 CNT 的羧基之间形成了共价键。确定了基于卵清蛋白（OVA）和牛血清白蛋白（BSA）的蛋白共轭物的浓度（0.3 和 0.2 mg/mL）以及抗体的稀释度（0.25），从而使压电免疫传感器具有最佳特性。确定了使用拟议方法测定 AA 的计量特性：使用基于 MCNC/AA-OVA 和 MCNC/AA-BSA 的带有识别层的压电免疫传感器测定 AA 的含量范围和检测限分别为 50-400 和 10 纳克/毫升，以及 100-300 和 50 纳克/毫升。对传感器进行了测试，以测定中草药茶和减肥膳食补充剂样品中的 AA 含量。在茶叶中没有发现酸，但在膳食补充剂中，其含量为 3.2 微克/克。

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来源期刊

Inorganic Materials 工程技术-材料科学：综合

CiteScore

1.40

自引率

25.00%

发文量

审稿时长

3-6 weeks

期刊介绍： Inorganic Materials is a journal that publishes reviews and original articles devoted to chemistry, physics, and applications of various inorganic materials including high-purity substances and materials. The journal discusses phase equilibria, including P–T–X diagrams, and the fundamentals of inorganic materials science, which determines preparatory conditions for compounds of various compositions with specified deviations from stoichiometry. Inorganic Materials is a multidisciplinary journal covering all classes of inorganic materials. The journal welcomes manuscripts from all countries in the English or Russian language.