Genomics and taxonomy of the glyphosate-degrading, copper-tolerant rhizospheric bacterium Achromobacter insolitus LCu2

Yelena V. Kryuchkova, Alexandra A. Neshko, Natalia E. Gogoleva, Alexander S. Balkin, Vera I. Safronova, Kristina Yu. Kargapolova, Elena I. Shagimardanova, Yuri V. Gogolev, Gennady L. Burygin
{"title":"Genomics and taxonomy of the glyphosate-degrading, copper-tolerant rhizospheric bacterium Achromobacter insolitus LCu2","authors":"Yelena V. Kryuchkova,&nbsp;Alexandra A. Neshko,&nbsp;Natalia E. Gogoleva,&nbsp;Alexander S. Balkin,&nbsp;Vera I. Safronova,&nbsp;Kristina Yu. Kargapolova,&nbsp;Elena I. Shagimardanova,&nbsp;Yuri V. Gogolev,&nbsp;Gennady L. Burygin","doi":"10.1007/s10482-024-01989-3","DOIUrl":null,"url":null,"abstract":"<div><p>A rhizosphere strain, <i>Achromobacter insolitus</i> LCu2, was isolated from alfalfa (<i>Medicago sativa</i> L.) roots. It was able to degrade of 50% glyphosate as the sole phosphorus source, and was found resistant to 10 mM copper (II) chloride, and 5 mM glyphosate–copper complexes. Inoculation of alfalfa seedlings and potato microplants with strain LCu2 promoted plant growth by 30–50%. In inoculated plants, the toxicity of the glyphosate–copper complexes to alfalfa seedlings was decreased, as compared with the noninoculated controls. The genome of <i>A. insolitus</i> LCu2 consisted of one circular chromosome (6,428,890 bp) and encoded 5843 protein genes and 76 RNA genes. Polyphasic taxonomic analysis showed that <i>A. insolitus</i> LCu2 was closely related to <i>A. insolitus</i> DSM23807<sup>T</sup> on the basis of the average nucleotide identity of the genomes of 22 type strains and the multilocus sequence analysis. Genome analysis revealed genes putatively responsible for (1) plant growth promotion (osmolyte, siderophore, and 1-aminocyclopropane-1-carboxylate deaminase biosynthesis and auxin metabolism); (2) degradation of organophosphonates (glyphosate oxidoreductase and multiple <i>phn</i> clusters responsible for the transport, regulation and C–P lyase cleavage of phosphonates); and (3) tolerance to copper and other heavy metals, effected by the CopAB–CueO system, responsible for the oxidation of copper (I) in the periplasm, and by the efflux Cus system. The putative catabolic pathways involved in the breakdown of phosphonates are predicted. <i>A. insolitus</i> LCu2 is promising in the production of crops and the remediation of soils contaminated with organophosphonates and heavy metals.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s10482-024-01989-3","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

A rhizosphere strain, Achromobacter insolitus LCu2, was isolated from alfalfa (Medicago sativa L.) roots. It was able to degrade of 50% glyphosate as the sole phosphorus source, and was found resistant to 10 mM copper (II) chloride, and 5 mM glyphosate–copper complexes. Inoculation of alfalfa seedlings and potato microplants with strain LCu2 promoted plant growth by 30–50%. In inoculated plants, the toxicity of the glyphosate–copper complexes to alfalfa seedlings was decreased, as compared with the noninoculated controls. The genome of A. insolitus LCu2 consisted of one circular chromosome (6,428,890 bp) and encoded 5843 protein genes and 76 RNA genes. Polyphasic taxonomic analysis showed that A. insolitus LCu2 was closely related to A. insolitus DSM23807T on the basis of the average nucleotide identity of the genomes of 22 type strains and the multilocus sequence analysis. Genome analysis revealed genes putatively responsible for (1) plant growth promotion (osmolyte, siderophore, and 1-aminocyclopropane-1-carboxylate deaminase biosynthesis and auxin metabolism); (2) degradation of organophosphonates (glyphosate oxidoreductase and multiple phn clusters responsible for the transport, regulation and C–P lyase cleavage of phosphonates); and (3) tolerance to copper and other heavy metals, effected by the CopAB–CueO system, responsible for the oxidation of copper (I) in the periplasm, and by the efflux Cus system. The putative catabolic pathways involved in the breakdown of phosphonates are predicted. A. insolitus LCu2 is promising in the production of crops and the remediation of soils contaminated with organophosphonates and heavy metals.

Abstract Image

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
草甘膦降解耐铜根瘤细菌 Achromobacter insolitus LCu2 的基因组学和分类学。
从紫花苜蓿(Medicago sativa L.)根中分离出根瘤菌 Achromobacter insolitus LCu2。它能降解作为唯一磷源的 50% 草甘膦,并对 10 mM 氯化铜 (II) 和 5 mM 草甘膦-铜复合物具有抗性。用菌株 LCu2 接种苜蓿幼苗和马铃薯微型植株,可促进植物生长 30-50%。与未接种的对照组相比,接种植物中草甘膦-铜复合物对苜蓿幼苗的毒性有所降低。A. insolitus LCu2 的基因组由一个环状染色体(6,428,890 bp)组成,编码 5843 个蛋白质基因和 76 个 RNA 基因。多相分类分析表明,根据 22 个类型菌株基因组的平均核苷酸同一性和多焦点序列分析,A. insolitus LCu2 与 A. insolitus DSM23807T 关系密切。基因组分析发现了可能负责以下方面的基因:(1) 促进植物生长(渗透剂、苷元和 1-aminocyclopropane-1-carboxylate deaminase 生物合成和辅酶代谢);(2) 有机膦酸盐的降解(草甘膦氧化还原酶和多个 phn 簇,负责膦酸盐的运输、调节和 C-P 裂解酶裂解);以及 (3) 对铜和其他重金属的耐受性,通过 CopAB-CueO 系统(负责铜(I)在周质中的氧化)和外流 Cus 系统来实现。预测了参与膦酸盐分解的假定代谢途径。A. insolitus LCu2 在农作物生产和修复受有机膦酸盐和重金属污染的土壤方面前景广阔。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
5.60
自引率
11.50%
发文量
104
审稿时长
3 months
期刊介绍: Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.
期刊最新文献
Description and genomic characterization of Mangrovibrevibacter kandeliae gen. nov. sp. nov., a novel carotenoid-producing endophytic bacterium isolated from branch of mangrove plants Jannaschia ovalis sp. nov., a marine bacterium isolated from a tidal flat sediment in the Republic of Korea Cerasicoccus fimbriatus sp. nov., isolated from the mid-ridge of the Southwest Indian Ocean A description of Joostella sp. strain CR20 with potential biotechnological applications Isoalcanivorax beigongshangi sp. nov., isolated from the fermented grains of Chinese baijiu
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1