Enhanced electrochemiluminescence of dual-defect graphite carbon nitride for ultrasensitive detection of CEA

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioelectrochemistry Pub Date : 2024-07-17 DOI:10.1016/j.bioelechem.2024.108781

Wenjie Chen , Xieyin Li , Minjia Tao , Hao Zhang , Qian Zhang , Yuchen Yue , Guifu Zuo , Junjian Zhao

引用次数: 0

Abstract

Herein, a dual-defective graphite carbon nitride (DDCN) was prepared by polymerization under N₂ atmosphere combined with oxidation treatment. The luminous intensity of dual-defect graphite phase carbon nitride based on defect state luminescence is significantly improved compared to CN-air. On this basis, a biosensor for CEA detection was constructed based on specific immunobinding of antigen–antibody. It is noted that the biosensor exhibits a wide linear range of 1 × 10^-5 ∼ 1 × 10² ng•mL⁻¹, a low detection limit of 3.3 × 10^-4 pg•mL⁻¹, a recovery of 94 %∼105 % and RSD less than 4.41 %. In addition, there was no significant difference to the clinical results, indicating that this work has good clinical application prospects.

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用于超灵敏检测癌胚抗原的双缺陷氮化石墨增强电化学发光。

本文通过在 N2 气氛下聚合并结合氧化处理制备了双缺陷石墨氮化碳（DDCN）。与 CN-空气相比，基于缺陷态发光的双缺陷石墨相氮化碳的发光强度显著提高。在此基础上，构建了一种基于抗原-抗体特异性免疫结合的 CEA 检测生物传感器。该生物传感器的线性范围为 1 × 10-5 ∼ 1 × 102 ng-mL-1，检测限低至 3.3 × 10-4 pg-mL-1，回收率为 94 % ∼ 105 %，RSD 小于 4.41 %。此外，与临床结果无明显差异，表明该工作具有良好的临床应用前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.