Effects of polystyrene microplastics on the extracellular and intracellular dissolved organic matter released by Skeletonema costatum using a novel in situ method

Abstract

Microplastics (MPs) affect the physicochemical algal-dissolved organic matter properties, indirectly influencing the environmental behavior of contaminants including persistent organic pollutants and heavy metals. Limited research is available on the roles played by intracellular- and extracellular-dissolved organic matter (I-DOM and E-DOM) in the processes that affect the environmental behavior of contaminants. Furthermore, the effects of MPs on the production of I-DOM and E-DOM, as well as their environmental behaviors, remain uncertain. A critical issue lies in the challenge of quantitatively identifying I-DOM and E-DOM in situ. In this work, a new fluorescence ratio method was developed and applied to in situ examine the impacts of polystyrene (PS) MPs (50, 500 nm, and 5 μm) on the I-DOM and E-DOM released by Skeletonema costatum (S. costatum). The experimental results indicated that the detection limits were 0.06 mg L⁻¹, with the respective minimum detectable proportions being 2% for both E-DOM and I-DOM. The suppressive effects of 10–50 mg L⁻¹ of 50 and 500 nm PS MPs on the cell proliferation of S. costatum and the E-DOM secretion were most pronounced on day 6. And the rates of suppression of E-DOM secretion were 10.1%–18.2% and 4.2%–13.9%, respectively. The exposure of algal cells to 50 mg L⁻¹ of 50 and 500 nm PS MPs led to cell rupture and the leakage of I-DOM on day 6. This suggests that the developed method in the laboratory could offer a promising approach for studying the generation of E-DOM and I-DOM in situ, as well as their environmental behaviors affected by MPs.