Comparative genomic and phylogenetic analysis of the complete mitochondrial genome of Cricula trifenestrata (Helfer) among lepidopteran insects.

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Genome Pub Date : 2024-11-01 Epub Date: 2024-07-24 DOI:10.1139/gen-2023-0037
Deepika Singh, Ponnala Vimal Mosahari, Pragya Sharma, Kartik Neog, Utpal Bora
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Abstract

Cricula trifenestrata Helfer (commonly known as Amphutukoni muga/Cricula silkworm), a wild sericigenous insect produces golden yellow silk similar to Antheraea assamensis (muga silkworm), with significant potential as a natural fiber and biomaterial. Cricula is considered as a pest as it competes for food with muga, which produces the prized golden silk. This study focuses on decoding the mitochondrial genome of C. trifenestrata using next-generation sequencing technology and includes comparative analysis with Bombycoids and other lepidopteran insects. We found that the Cricula mitogenome spans 15 425 bp and exhibits typical gene content and arrangement consistent with other Saturniids and lepidopterans. All protein-coding genes were found to undergo purifying selection, with the highest and lowest conservation observed in the cox1 and atp8 gene, respectively, indicating their potential role in future evolutionary events. We identified two types of mismatches: 23 "G-U" and 6 "U-U" pairs, similar to those found in Actias selene among the Saturniids. Additionally, our study uncovered the presence of two 33 bp repeat units and a "TTAGA" motif in the control region, in contrast to the typical "ATAGA" motif, suggesting functional similarity with evolving sequences. Furthermore, phylogenetic analysis supports the close relationship of Cricula with other species within the Saturniidae family.

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鳞翅目昆虫中 Cricula trifenestrata (Helfer) 完整线粒体基因组的比较基因组学和系统发生学分析。
Cricula trifenestrata Helfer(俗称 Amphutukoni muga/Cricula蚕)是一种野生蚕媒昆虫,可生产与 Antheraea assamensis(Muga蚕)相似的金黄色丝绸,具有作为天然纤维和生物材料的巨大潜力。蟋蟀茧蚕被视为一种害虫,因为它要与生产珍贵金丝的穆加蚕争夺食物。本研究的重点是利用新一代测序技术解码 C. trifenestrata 的线粒体基因组,包括与 Bombycoids 和其他鳞翅目昆虫的比较分析。我们发现,蟋蟀有丝分裂基因组的长度为 15,425 bp,其基因含量和排列方式与其他土星目昆虫和鳞翅目昆虫一致。我们发现所有蛋白质编码基因都经历了纯化选择,其中 cox1 和 atp8 基因的保护程度分别最高和最低,这表明它们在未来的进化事件中可能发挥作用。我们发现了两种类型的错配:我们发现了两类错配:23 对 "G-U "和 6 对 "U-U",这与土星人中的 Actias selene 发现的错配相似。此外,我们的研究还发现在控制区存在两个 33 bp 的重复单元和一个 "TTAGA "图案,与典型的 "ATAGA "图案不同,这表明与进化序列存在功能上的相似性。此外,系统发育分析还支持蟋蟀与土鳖虫科其他物种的密切关系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Genome
Genome 生物-生物工程与应用微生物
CiteScore
5.30
自引率
3.20%
发文量
42
审稿时长
6-12 weeks
期刊介绍: Genome is a monthly journal, established in 1959, that publishes original research articles, reviews, mini-reviews, current opinions, and commentaries. Areas of interest include general genetics and genomics, cytogenetics, molecular and evolutionary genetics, developmental genetics, population genetics, phylogenomics, molecular identification, as well as emerging areas such as ecological, comparative, and functional genomics.
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