ABCA1 Deletion Does Not Affect Aqueous Humor Outflow Function in Mice.

IF 1.8 4区 医学 Q3 OPHTHALMOLOGY Journal of Ophthalmology Pub Date : 2024-07-17 eCollection Date: 2024-01-01 DOI:10.1155/2024/7195550
Bo Qin, Chunchun Hu, Youjia Zhang, Yuhong Chen, Yuan Lei
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Abstract

Background: ATP binding cassette transporter A1 (ABCA1) is a candidate gene within a POAG susceptibility locus by GWAS analysis, and it is involved in IOP modulation via the Cav1/eNOS/NO signaling pathway. We aim to examine the phenotype of ABCA1 deletion in the ABCA1 gene knockout (Abca1-/-) mice.

Methods: The anterior segments of Abca1-/- eyes were imaged by slit-lamp microscopy and anterior segment OCT. IOPs were measured by rebound tonometry. By perfusing enucleated eyes at various pressures, the aqueous humor outflow facility was determined. The mRNA expressions of ABCA1, Cav1, and eNOS were measured by RT-qPCR. The protein expressions were analyzed by western blot and immunofluorescence staining.

Results: There was no significant difference in the anterior segment morphology of Abca1-/- mice. IOP and aqueous humor outflow facility did not change in Abca1-/- mice compared with wild-type mice. mRNA and protein expressions of ABCA1 were significantly lower in the outflow tissue of Abca1-/- eyes. The expressions of Cav1 and eNOS were both significantly upregulated in the outflow tissue of Abca1-/- eyes.

Conclusion: ABCA1 deletion does not affect IOP and aqueous humor outflow function but the Cav1/eNOS/NO pathway is changed in Abca1-/- mice. The function of ABCA1 in aqueous humor outflow still requires further research.

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ABCA1 缺失不会影响小鼠的眼液外流功能
背景:通过GWAS分析,ATP结合盒转运体A1(ABCA1)是POAG易感基因位点的候选基因,它通过Cav1/eNOS/NO信号通路参与眼压调节。我们旨在研究 ABCA1 基因敲除(Abca1-/-)小鼠中 ABCA1 缺失的表型:方法:用裂隙灯显微镜和前节 OCT 对 Abca1-/- 眼睛的前节进行成像。通过回弹式眼压计测量眼压。在不同压力下灌注去核眼球,测定房水外流设施。通过 RT-qPCR 检测 ABCA1、Cav1 和 eNOS 的 mRNA 表达。蛋白质表达采用 Western 印迹和免疫荧光染色法进行分析:结果:Abca1-/-小鼠的前节形态无明显差异。ABCA1的mRNA和蛋白表达在Abca1-/-小鼠眼球流出组织中明显降低。Cav1和eNOS在Abca1-/-眼流出组织中的表达均明显上调:结论:ABCA1缺失不会影响眼压和房水流出功能,但Abca1-/-小鼠的Cav1/eNOS/NO通路发生了变化。ABCA1在房水外流中的功能仍有待进一步研究。
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来源期刊
Journal of Ophthalmology
Journal of Ophthalmology MEDICINE, RESEARCH & EXPERIMENTAL-OPHTHALMOLOGY
CiteScore
4.30
自引率
5.30%
发文量
194
审稿时长
6-12 weeks
期刊介绍: Journal of Ophthalmology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies related to the anatomy, physiology and diseases of the eye. Submissions should focus on new diagnostic and surgical techniques, instrument and therapy updates, as well as clinical trials and research findings.
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