Transdermal delivery system to release phthalocyanine photosensitizers for the potential treatment of skin cancer with PDT.

IF 1.3 4区化学 Q3 CHEMISTRY, MULTIDISCIPLINARY Turkish Journal of Chemistry Pub Date : 2024-03-12 eCollection Date: 2024-01-01 DOI:10.55730/1300-0527.3665

Meryem Çamur Demir, Fatma Kurşun Baysak, Caner Yahya Boyar, Alihan Toksoy, Fatih Algi

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Abstract

This research aims to examine the transdermal release of water-soluble indium and zinc metallo phthalocyanine (InPc and ZnPc) compounds from the poly(vinyl alcohol) (PVA) membrane and the cytotoxicity effect of these Pcs on normal mouse fibroblasts (L929 fibroblast) and human melanoma (SK-MEL-30) cells. For this purpose, the effects of temperature, pH, drug concentration and membrane thickness on transdermal release were investigated in order to obtain the optimum transdermal release profile by preparing PVA membranes with different thicknesses and crosslinked by heat treatment. Optimum drug release was found to be 85.36% using 6 μm thick PVA membrane at 37 ± 0.5 °C, when upper cell pH 1.2 and lower cell pH 5.5, for 3 mg/mL InPc drug concentration. Under the same conditions, the drug release value for ZnPc was found to be 69.78%. In addition, in vitro studies were performed on L929 and SK-MEL-30 cells. under optimized drug (InPc and ZnPc) and membrane conditions. It was found that no significant cytotoxic effect was observed in L929 and SK-MEL-30 cells in the dark. Photodynamic tests were also carried out with InPc and ZnPc. The results show that cell viability decreases in SK-MEL-30 cells at concentrations of 10 μg/mL and above. In addition, while the InPc IC50 value was determined as 4.058 μg/mL, this value was determined as 11.574 μg/mL for ZnPc.

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释放酞菁光敏剂的透皮给药系统，可用于皮肤癌的局部光疗。

本研究旨在考察水溶性铟和锌金属酞菁（InPc 和 ZnPc）化合物从聚（乙烯醇）（PVA）膜中的透皮释放以及这些 Pcs 对正常小鼠成纤维细胞（L929 成纤维细胞）和人类黑色素瘤（SK-MEL-30）细胞的细胞毒性作用。为此，研究了温度、pH 值、药物浓度和膜厚度对透皮释放的影响，以便通过制备不同厚度并经热处理交联的 PVA 膜获得最佳透皮释放曲线。结果发现，在 37 ± 0.5 °C、上层细胞 pH 值为 1.2、下层细胞 pH 值为 5.5、药物浓度为 3 mg/mL InPc 的条件下，6 μm 厚的 PVA 膜的最佳药物释放率为 85.36%。在相同条件下，ZnPc 的药物释放值为 69.78%。此外，在优化的药物（InPc 和 ZnPc）和膜条件下，还对 L929 和 SK-MEL-30 细胞进行了体外研究。结果发现，在黑暗条件下，L929 和 SK-MEL-30 细胞没有观察到明显的细胞毒性效应。还用 InPc 和 ZnPc 进行了光动力试验。结果表明，当浓度为 10 μg/mL 及以上时，SK-MEL-30 细胞的细胞活力会下降。此外，InPc 的 IC50 值被确定为 4.058 微克/毫升，而 ZnPc 的 IC50 值被确定为 11.574 微克/毫升。

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来源期刊

Turkish Journal of Chemistry 化学-工程：化工

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

3 months

期刊介绍： The Turkish Journal of Chemistry is a bimonthly multidisciplinary journal published by the Scientific and Technological Research Council of Turkey (TÜBİTAK). The journal is dedicated to dissemination of knowledge in all disciplines of chemistry (organic, inorganic, physical, polymeric, technical, theoretical and analytical chemistry) as well as research at the interface with other sciences especially in chemical engineering where molecular aspects are key to the findings. The journal accepts English-language original manuscripts and contribution is open to researchers of all nationalities. The journal publishes refereed original papers, reviews, letters to editor and issues devoted to special fields. All manuscripts are peer-reviewed and electronic processing ensures accurate reproduction of text and data, plus publication times as short as possible.