Identification of Alpha-Gal glycolipids in saliva of Lone-Star Tick (Amblyomma americanum)

IF 3.1 2区医学 Q2 INFECTIOUS DISEASES Ticks and Tick-borne Diseases Pub Date : 2024-07-24 DOI:10.1016/j.ttbdis.2024.102384

Surendra Raj Sharma , Sabir Hussain , Shailesh K. Choudhary , Scott P. Commins , Shahid Karim

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Abstract

Alpha-Gal Syndrome (AGS) is a delayed allergic reaction triggered by IgE antibodies targeting galactose-α-1,3-galactose (α-gal), prevalent in red meat. Its global significance has increased, with over 450,000 estimated cases in the United States alone. AGS is linked to tick bites, causing sensitization and elevated α-gal specific IgE levels. However, the precise mechanisms and tick intrinsic factors contributing to AGS development post-tick bites remain unclear. This study aims to characterize the alpha-gal conjugated lipid antigens in Amblyomma americanum (Am. americanum) salivary glands and saliva. Nanospray ionization mass spectrometry (NSI-MS) analysis revealed the identification of α-gal bound lipid antigens in Am. americanum saliva. Additionally, the activation of basophils by extracted alpha-gal bound lipids and proteins provides evidence of their antigenic capabilities.

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龙星虱（Amblyomma americanum）唾液中 Alpha-Gal 糖脂的鉴定。

α-gal综合征（AGS）是一种由针对红肉中常见的半乳糖-α-1,3-半乳糖（α-gal）的IgE抗体引发的迟发性过敏反应。这种疾病在全球的发病率不断上升，仅在美国估计就有超过 45 万例病例。AGS 与蜱虫叮咬有关，会导致过敏和 α-gal 特异性 IgE 水平升高。然而，蜱虫叮咬后导致 AGS 发生的确切机制和蜱虫内在因素仍不清楚。本研究旨在描述美洲蜱（Amblyomma americanum）唾液腺和唾液中的α-gal共轭脂质抗原的特征。纳米喷雾电离质谱（NSI-MS）分析表明，在美洲大鲵唾液中发现了与α-gal结合的脂质抗原。此外，提取的α-gal结合脂质和蛋白质对嗜碱性粒细胞的激活作用也证明了它们的抗原能力。

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来源期刊

Ticks and Tick-borne Diseases INFECTIOUS DISEASES-MICROBIOLOGY

CiteScore

6.90

自引率

12.50%

发文量

185

审稿时长

6-12 weeks

期刊介绍： Ticks and Tick-borne Diseases is an international, peer-reviewed scientific journal. It publishes original research papers, short communications, state-of-the-art mini-reviews, letters to the editor, clinical-case studies, announcements of pertinent international meetings, and editorials. The journal covers a broad spectrum and brings together various disciplines, for example, zoology, microbiology, molecular biology, genetics, mathematical modelling, veterinary and human medicine. Multidisciplinary approaches and the use of conventional and novel methods/methodologies (in the field and in the laboratory) are crucial for deeper understanding of the natural processes and human behaviour/activities that result in human or animal diseases and in economic effects of ticks and tick-borne pathogens. Such understanding is essential for management of tick populations and tick-borne diseases in an effective and environmentally acceptable manner.