Autologous serum protein stabilized silver quantum clusters as host-specific antibacterial agents.

Nanomedicine (London, England) Pub Date : 2024-01-01 Epub Date: 2024-07-26 DOI:10.1080/17435889.2024.2374231

Kritika Sood, Asifkhan Shanavas

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Abstract

Aim: To synthesize host-specific serum protein stabilized silver quantum clusters and assess their preclinical safety as potential antibacterial agents.Materials & methods: Ag-QC-NanoSera (Ag-QCNS) were synthesized using bovine, human and murine sera. Antibacterial efficacy was evaluated against E. coli (including antibiotic-resistant strain), S. aureus and P. aeruginosa. Biocompatibility, hemocompatibility and antibacterial mechanism were also investigated. Preclinical safety and biodistribution of autologous Ag-QCNS were assessed in BALB/c mice over 28 days.Results: Ag-QCNS showed high biocompatibility, hemocompatibility and high antibacterial activity at ∼12.72 μg/ml Ag equivalent. Intracellular ROS and bacterial membrane damage were confirmed as antibacterial mechanism. Ag-QCNS were established as preclinically safe.Conclusion: Ag-QCNS demonstrate potential as next-generation host-specific nanotheranostic antibacterial agents, enhancing the safety and efficacy while combating antibiotic resistance.

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作为宿主特异性抗菌剂的自体血清蛋白稳定银量子簇。

目的：合成宿主特异性血清蛋白稳定银量子团簇，并评估其作为潜在抗菌剂的临床前安全性。材料与方法：使用牛、人和鼠血清合成 Ag-QC-NanoSera (Ag-QCNS)。对大肠杆菌（包括抗生素耐药菌株）、金黄色葡萄球菌和绿脓杆菌的抗菌效果进行了评估。此外，还研究了生物相容性、血液相容性和抗菌机制。在 BALB/c 小鼠中对自体 Ag-QCNS 的临床前安全性和生物分布进行了 28 天的评估。结果显示Ag-QCNS具有很高的生物相容性、血液相容性和抗菌活性，Ag当量为12.72 μg/ml。细胞内 ROS 和细菌膜损伤被证实为抗菌机制。Ag-QCNS在临床前是安全的。结论Ag-QCNS具有作为下一代宿主特异性纳米otheranostic抗菌剂的潜力，在提高安全性和有效性的同时还能对抗抗生素耐药性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Nanomedicine (London, England)

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