Waterlogging-Induced Changes in ABA, Carbohydrates and CBF6 Modify Freezing Tolerance in Prehardened Festuca pratensis

IF 3.7 2区 农林科学 Q1 AGRONOMY Journal of Agronomy and Crop Science Pub Date : 2024-07-24 DOI:10.1111/jac.12740
Barbara Jurczyk, Michał Dziurka, Franciszek Janowiak, Ewa Pociecha, Maciej Grzesiak, Marcin Rapacz
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Abstract

Changes in precipitation and snow melt during warmer winters can increase low-temperature waterlogging. Such conditions may bring about different effects when compared with a single stress trigger, such as low-temperature or water excess. The effects of waterlogging are clearly related to water temperature, and the consequences of water excess might be less severe, as more oxygen is dissolved in colder water. The effect of waterlogging during cold acclimation (CA) is poorly understood; most experiments concerning water excess are performed at relatively high-temperatures. In this study, we examined the effect of 3 weeks of waterlogging (approx. 2 cm above the soil level) on CA in Festuca pratensis Huds. (Fp), a cool-season grass. Measurements were taken before CA (after prehardening, before flooding) and after 3 weeks of CA in waterlogged (treated) and non-waterlogged (control) plants. The work included: (i) freezing tolerance test (regrowth after freezing), (ii) analysis of abscisic acid (ABA) content in the leaf, (iii) leaf stomatal conductance, (iv) leaf water content, (v) carbohydrates analysis, including fructans, and (vi) transcript levels of selected genes involved in freezing tolerance, ABA signalling and fructan biosynthesis. The aim of the study was to test a hypothesis that low-temperature waterlogging in Fp enhances freezing tolerance (plant regrowth after freezing) related to increased ABA accumulation, increased C-repeat-binding transcription factor expression and/or increased carbohydrate accumulation, including fructans. Two out of four genotypes exhibited enhanced regrowth following freezing due to waterlogging relative to control. Principal component analysis (PCA) revealed a positive correlation between ABA levels and freezing tolerance in both treatments, with a more pronounced effect observed in the waterlogged plants. However, the phytohormone played different roles in these two treatments. In the context of low-temperature waterlogging, ABA may be involved in the dehydration tolerance response in genotypes suffering from physiological drought, as well as the induction of C-repeat-binding transcription factors (CBFs) and sucrose, which may improve freezing tolerance. The increased fructan amount and polymerisation degree due to waterlogging may provide a carbohydrate sink to maintain a high photosynthetic efficiency, but are not directly responsible for freezing tolerance changes. The study indicates that tolerance mechanisms of Fp exposed to low-temperature waterlogging involve maintaining a high photosynthetic rate, as well as oxidative and dehydration stress tolerance.

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水涝引起的 ABA、碳水化合物和 CBF6 变化改变了预硬苗的耐冻能力
冬季变暖时降水和融雪的变化会加剧低温涝害。与低温或水量过多等单一压力触发因素相比,这些条件可能会带来不同的影响。水涝的影响显然与水温有关,而水量过多的后果可能没那么严重,因为较冷的水中溶解了更多的氧气。人们对低温适应(CA)过程中水涝的影响知之甚少;大多数有关水过量的实验都是在相对较高的温度下进行的。在这项研究中,我们考察了涝害 3 周(高出土壤水平约 2 厘米)对 Festuca pratensis Huds.(Fp)(一种冷季型草坪草)CA 的影响。在 CA 之前(预硬化后,淹水前)和 CA 3 周后,分别对水涝(处理)和非水涝(对照)植物进行了测量。工作包括(i)耐冻性测试(受冻后重新生长),(ii)叶片中脱落酸(ABA)含量分析,(iii)叶片气孔导度,(iv)叶片含水量,(v)碳水化合物分析,包括果聚糖,以及(vi)参与耐冻性、ABA 信号传导和果聚糖生物合成的选定基因的转录水平。该研究旨在验证一个假设,即 Fp 的低温涝害会增强耐冻性(植物受冻后重新生长),这与 ABA 积累增加、C-重复结合转录因子表达增加和/或碳水化合物(包括果聚糖)积累增加有关。与对照组相比,四种基因型中有两种在受冻后由于涝害表现出更强的再生能力。主成分分析(PCA)显示,在两种处理中,ABA 水平与耐冻性之间存在正相关,在受涝植株中观察到的影响更为明显。不过,植物激素在这两种处理中的作用不同。在低温涝害的情况下,ABA 可能参与了生理干旱基因型的脱水耐受反应,也可能诱导了 C 重复结合转录因子(CBFs)和蔗糖,从而提高了抗冻性。涝害导致的果聚糖数量和聚合度增加可能为维持高光合效率提供了碳水化合物汇,但并不是耐冻性变化的直接原因。该研究表明,Fp 对低温涝害的耐受机制包括维持高光合速率以及耐氧化和脱水胁迫。
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来源期刊
Journal of Agronomy and Crop Science
Journal of Agronomy and Crop Science 农林科学-农艺学
CiteScore
8.20
自引率
5.70%
发文量
54
审稿时长
7.8 months
期刊介绍: The effects of stress on crop production of agricultural cultivated plants will grow to paramount importance in the 21st century, and the Journal of Agronomy and Crop Science aims to assist in understanding these challenges. In this context, stress refers to extreme conditions under which crops and forages grow. The journal publishes original papers and reviews on the general and special science of abiotic plant stress. Specific topics include: drought, including water-use efficiency, such as salinity, alkaline and acidic stress, extreme temperatures since heat, cold and chilling stress limit the cultivation of crops, flooding and oxidative stress, and means of restricting them. Special attention is on research which have the topic of narrowing the yield gap. The Journal will give preference to field research and studies on plant stress highlighting these subsections. Particular regard is given to application-oriented basic research and applied research. The application of the scientific principles of agricultural crop experimentation is an essential prerequisite for the publication. Studies based on field experiments must show that they have been repeated (at least three times) on the same organism or have been conducted on several different varieties.
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