No tillage and organic fertilization improved kiwifruit productivity through shifting soil properties and microbiome

Abstract

The preservation of edaphic quality and productivity is critical for the ecological sustainability of vine orchards. The heavy utilization of intensified tillage and singular chemical fertilizers can shift changes in edaphic physicochemical and biological features, thus exerting significant pressure on agroecosystems. In current research, we assessed the shifts in soil physicochemical features and soil microbiome composition over 11 years carrying out no tillage and organic fertilizer substitution in a typical Chinese Guanzhong kiwifruit production area, and explore the fundamental factors that contribute to alterations in the microbial community and the influence on kiwifruit performance. Results showed that long-term no tillage and organic fertilizer improved the soil condition by significantly increasing the proportion of soil macroaggregates, bulk density, and nutrient content (e.g., organic matter, nitrogen, and ammonia), as compared to conventional tillage with chemical fertilization. Moreover, no tillage significantly increased soil bacterial α-diversity but had no significant effects on fungal. No tillage also enhanced the abundance of potential beneficial soil bacteria (e.g., Acidobacteria, Actinobacteria, and Nitrospira), while decreasing the abundance of Proteobacteria, Pseudomonas, and Fusarium. In addition, no tillage and mixed fertilized soil microbial network exhibited higher complexity (i.e., node and edge numbers, and positive edge proportion) and connectivity (i.e., average number of neighbors) than conventional tillage and chemical fertilization group. Changes in nitrate, ammonia, available phosphorus, and pH values accounted for the variation in the structure of soil microbial community. Hence, the utilization of both no tillage and organic fertilization practices could serve as a suitable and sustainable approach for managing kiwifruit production in the fragile environmental conditions of the Chinese Guanzhong region, and lead to an improvement in soil nutrient levels and help regulate the soil microbial community.