Continuous flow delivery system for the perfusion of scaffold-based 3D cultures†

IF 6.1 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS Lab on a Chip Pub Date : 2024-07-26 DOI:10.1039/D4LC00480A
Zachary R. Sitte, Elizabeth E. Karlsson, Haolin Li, Haibo Zhou and Matthew R. Lockett
{"title":"Continuous flow delivery system for the perfusion of scaffold-based 3D cultures†","authors":"Zachary R. Sitte, Elizabeth E. Karlsson, Haolin Li, Haibo Zhou and Matthew R. Lockett","doi":"10.1039/D4LC00480A","DOIUrl":null,"url":null,"abstract":"<p >The paper-based culture platform developed by Whitesides readily incorporates tissue-like structures into laboratories with established workflows that rely on monolayer cultures. Cell-laden hydrogels are deposited in these porous scaffolds with micropipettes; these scaffolds support the thin gel slabs, allowing them to be evaluated individually or stacked into thick constructs. The paper-based culture platform has inspired many basic and translational studies, each exploring how readily accessible materials can generate complex structures that mimic aspects of tissues <em>in vivo</em>. Many of these examples have relied on static culture conditions, which result in diffusion-limited environments and cells experiencing pericellular hypoxia. Perfusion-based systems can alleviate pericellular hypoxia and other cell stresses by continually exposing the cells to fresh medium. These perfusion systems are common in microfluidic and organ-on-chip devices supporting cells as monolayer cultures or as 3D constructs. Here, we introduce a continuous flow delivery system, which uses parts readily produced with 3D printing to provide a self-contained culture platform in which cells in paper or other scaffolds are exposed to fresh (flowing) medium. We demonstrate the utility of this device with examples of cells maintained in single cell-laden scaffolds, stacks of cell-laden scaffolds, and scaffolds that contain monolayers of endothelial cells. These demonstrations highlight some possible experimental questions that can be enabled with readily accessible culture materials and a perfusion-based device that can be readily fabricated.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":null,"pages":null},"PeriodicalIF":6.1000,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lab on a Chip","FirstCategoryId":"5","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2024/lc/d4lc00480a","RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0

Abstract

The paper-based culture platform developed by Whitesides readily incorporates tissue-like structures into laboratories with established workflows that rely on monolayer cultures. Cell-laden hydrogels are deposited in these porous scaffolds with micropipettes; these scaffolds support the thin gel slabs, allowing them to be evaluated individually or stacked into thick constructs. The paper-based culture platform has inspired many basic and translational studies, each exploring how readily accessible materials can generate complex structures that mimic aspects of tissues in vivo. Many of these examples have relied on static culture conditions, which result in diffusion-limited environments and cells experiencing pericellular hypoxia. Perfusion-based systems can alleviate pericellular hypoxia and other cell stresses by continually exposing the cells to fresh medium. These perfusion systems are common in microfluidic and organ-on-chip devices supporting cells as monolayer cultures or as 3D constructs. Here, we introduce a continuous flow delivery system, which uses parts readily produced with 3D printing to provide a self-contained culture platform in which cells in paper or other scaffolds are exposed to fresh (flowing) medium. We demonstrate the utility of this device with examples of cells maintained in single cell-laden scaffolds, stacks of cell-laden scaffolds, and scaffolds that contain monolayers of endothelial cells. These demonstrations highlight some possible experimental questions that can be enabled with readily accessible culture materials and a perfusion-based device that can be readily fabricated.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
用于灌注基于支架的三维培养物的连续流输送系统
Whitesides开发的纸基培养平台可轻松将组织样结构纳入依赖单层培养的实验室既定工作流程中。含有细胞的水凝胶通过微量移液管沉积在这些多孔支架上;这些支架支撑着薄凝胶板,使它们可以单独评估或堆叠成厚的结构。基于纸张的培养平台激发了许多基础研究和转化研究的灵感,每项研究都在探索容易获得的材料如何生成复杂的结构,以模仿体内组织的各个方面。其中许多例子都依赖于静态培养条件,这会导致扩散受限的环境和细胞周缺氧。基于灌流的系统可以通过不断让细胞接触新鲜培养基来缓解细胞周围缺氧和其他细胞压力。这些灌流系统常见于支持单层培养细胞或三维构建细胞的微流控和芯片上器官装置。在这里,我们介绍了一种连续流输送系统,它使用三维打印技术生产的部件,提供了一个独立的培养平台,使纸或其他支架中的细胞暴露在新鲜(流动)的培养基中。我们举例说明了这种装置的实用性,包括在单个细胞支架、多层细胞支架和含有单层内皮细胞的支架中培养细胞。这些演示强调了一些可能的实验问题,这些问题可以通过容易获得的培养材料和容易制造的基于灌流的设备来解决。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Lab on a Chip
Lab on a Chip 工程技术-化学综合
CiteScore
11.10
自引率
8.20%
发文量
434
审稿时长
2.6 months
期刊介绍: Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.
期刊最新文献
Observing root growth and signalling responses to stress gradients and pathogens using the bi-directional dual-flow RootChip Optical tweezer-assisted cell pairing and fusion for somatic cell nuclear transfer within an open microchannel. Microstring-engineered tension tissues: A novel platform for replicating tissue mechanics and advancing mechanobiology Discretised microfluidics for noninvasive health monitoring using sweat sensing Inside back cover
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1