Exogenous melatonin delays yellowing in harvested broccoli by maintaining chloroplast integrity

IF 5.7 1区农林科学 Q1 HORTICULTURE Horticultural Plant Journal Pub Date : 2024-07-20 DOI:10.1016/j.hpj.2023.12.012

Ran Yan, Mariama Kebbeh, Yuan Cheng, Yue Wang, Liu Yan, Chen Huan, Xiaolin Zheng, Shuling Shen

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Abstract

Yellowing of broccoli is a crucial limiting factor for its commercial value and consumer acceptance during postharvest. In this study, the impacts of exogenous melatonin (MEL) on chlorophyll content and fluorescence, as well as ultrastructure and membrane lipid metabolism of chloroplasts in broccoli were investigated during postharvest. The results showed that MEL treatment (200 μmol L) maintained the chlorophyll content, chloroplast autofluorescence and integral structure, and reduced the level ofserotonin in the chloroplasts in broccoli. Also, MEL treatment inhibited the membrane lipid peroxidation of chloroplasts, as indicated by low levels of superoxide anion (O), hydrogen peroxide (HO) and malondialdehyde (MDA), and high levels of endogenous MEL. In addition, the stability and fluidity of chloroplast membranes were also better maintained in the treated broccoli via increasing the contents of phosphatidylglyceroland (PG), monogalactosyldiglyceride (MGDG), digalactosyldiglyceride (DGDG) and unsaturated fatty acids as well as decreasing saturated fatty acid content and the activities of lipoxygenase (LOX) and lipase (LPS). Thus, the application of MEL facilitated the maintenance of chloroplast integrity, thus contributing to yellowing postponement and the extension of the storage life of broccoli.

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外源性褪黑激素通过维持叶绿体完整性延缓收获西兰花的黄化过程

西兰花黄化是影响其商业价值和消费者接受度的一个重要限制因素。本研究调查了外源褪黑素（MEL）对西兰花采后叶绿素含量和荧光以及叶绿体超微结构和膜脂代谢的影响。结果表明，MEL 处理（200 μmol L）保持了西兰花叶绿素含量、叶绿体自发荧光和整体结构，并降低了叶绿体中的血清素水平。同时，MEL 处理可抑制叶绿体膜脂质过氧化，表现为超氧阴离子（O）、过氧化氢（HO）和丙二醛（MDA）含量低，内源 MEL 含量高。此外，通过增加磷脂酰甘油（PG）、单半乳糖酰甘油（MGDG）、二半乳糖酰甘油（DGDG）和不饱和脂肪酸的含量，以及降低饱和脂肪酸含量和脂氧合酶（LOX）与脂肪酶（LPS）的活性，处理后的西兰花也能更好地保持叶绿体膜的稳定性和流动性。因此，施用 MEL 有助于保持叶绿体的完整性，从而有助于推迟西兰花变黄和延长其贮藏期。

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来源期刊

Horticultural Plant Journal Environmental Science-Ecology

CiteScore

9.60

自引率

14.00%

发文量

293

审稿时长

33 weeks

期刊介绍： Horticultural Plant Journal (HPJ) is an OPEN ACCESS international journal. HPJ publishes research related to all horticultural plants, including fruits, vegetables, ornamental plants, tea plants, and medicinal plants, etc. The journal covers all aspects of horticultural crop sciences, including germplasm resources, genetics and breeding, tillage and cultivation, physiology and biochemistry, ecology, genomics, biotechnology, plant protection, postharvest processing, etc. Article types include Original research papers, Reviews, and Short communications.