{"title":"Phylogenetic Analysis of phn Transporters of Achromobacter insolitus LCu2","authors":"Ye. V. Kryuchkova, G. L. Burygin","doi":"10.1134/s0026261723605122","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Phosphonates are alternative phosphorus sources for bacteria. The genome of <i>Achromobacter insolitus</i> strain LCu2 contains three predicted <i>phn</i> clusters of ABC-type phosphonate transporters into the cell. To understand the functional, evolutionary, and ecological role of the <i>phn</i> clusters, phylogenetic analysis of substrate-binding PhnD proteins from strain LCu2 with their homologs in other <i>Achromobacter</i> species and in closely related genera of the family <i>Alcaligenaceae</i> was carried out. The PhnD transporters formed three separate clusters, which indicated the differences in their structural composition. PhnD<sub>1</sub> and PhnD<sub>2</sub> were present in the genomes of all <i>Achromobacter</i> species and grouped separately from those of other members of the family <i>Alcaligenaceae</i>, which indicated vertical inheritance of the <i>phn</i>D<sub>1</sub> and <i>phn</i>D<sub>2</sub> genes and their involvement in the life-supporting processes. <i>Phn</i>D<sub>3</sub> was found in the genomes of seven <i>Achromobacter</i> species. The <i>phn</i>D<sub>3</sub> gene was probably acquired via horizontal transfer or duplication and is induced during adaptation to changing environmental conditions. Maintenance of three structurally different <i>phn</i> clusters of transporters is probably ecologically advantageous to <i>A. insolitus</i> LCu2, providing for phosphorus retrieval from synthetic and natural organophosphonates as well as other sources.</p>","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s0026261723605122","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
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Abstract
Phosphonates are alternative phosphorus sources for bacteria. The genome of Achromobacter insolitus strain LCu2 contains three predicted phn clusters of ABC-type phosphonate transporters into the cell. To understand the functional, evolutionary, and ecological role of the phn clusters, phylogenetic analysis of substrate-binding PhnD proteins from strain LCu2 with their homologs in other Achromobacter species and in closely related genera of the family Alcaligenaceae was carried out. The PhnD transporters formed three separate clusters, which indicated the differences in their structural composition. PhnD1 and PhnD2 were present in the genomes of all Achromobacter species and grouped separately from those of other members of the family Alcaligenaceae, which indicated vertical inheritance of the phnD1 and phnD2 genes and their involvement in the life-supporting processes. PhnD3 was found in the genomes of seven Achromobacter species. The phnD3 gene was probably acquired via horizontal transfer or duplication and is induced during adaptation to changing environmental conditions. Maintenance of three structurally different phn clusters of transporters is probably ecologically advantageous to A. insolitus LCu2, providing for phosphorus retrieval from synthetic and natural organophosphonates as well as other sources.
期刊介绍：
Microbiology is an is an international peer reviewed journal that covers a wide range of problems in the areas of fundamental and applied microbiology. The journal publishes experimental and theoretical papers, reviews on modern trends in different fields of microbiological science, and short communications with descriptions of unusual observations. The journal welcomes manuscripts from all countries in the English or Russian language.
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