Comparative Study of Dispersive Micro-Solid Phase Extraction of Nandrolone Using MIL-53(Al) and ZIF-8 in Human Plasma

IF 1 4区化学 Q4 CHEMISTRY, ANALYTICAL Journal of Analytical Chemistry Pub Date : 2024-07-23 DOI:10.1134/s1061934824700278

Somaye Akbari, Azra Takhvar, Effat Souri, Reza Ahmadkhaniha, Ali Morsali, Mohammad reza Khoshayand, Mohsen Amini, Alireza Taheri

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Abstract

The extraction of nandrolone as a steroid hormone using fast methods is of high importance. This was done by dispersive micro-solid phase extraction in the presence of two widely used organometallic frameworks (MIL-53(Al) and ZIF-8). The extraction steps were optimized by an extensive study on the main factors affecting the absorption/desorption efficiency. The optimum conditions were obtained by design of experiments for the extraction and determination of nandrolone in a blank human plasma matrix. Maximum extraction of nandrolone was achieved in 8.5 min by 2.5 mg of MIL-53(Al) in the solution with 0.89% (w/v) salt concentration, while the maximum extraction by ZIF-8 as another sorbent required 10 min, 4.5 mg of sorbent, and 0.60% (w/v) of salt concentration. Batch experiments were carried out to find appropriate kinetic and isotherm models. Finally, the analytical validation of the proposed method was investigated. The dynamic range of the method was from 0.05 to 1 µg/mL. The precision (expressed as the relative standard deviation, RSD) and accuracy (expressed as the percentage error) of extraction by MIL-53(Al) were found 3.90 and 3.39%, respectively, while those by ZIF-8 were calculated as 4.59 and 4.50%, respectively. The proposed method was successfully applied for the determination of nandrolone in spiked samples, achieving high recovery rates of approximately 96% and 94% using MIL-53(Al) and ZIF-8.

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使用 MIL-53(Al) 和 ZIF-8 对人血浆中诺龙进行分散微固相萃取的比较研究

摘要采用快速方法提取甾体激素诺龙具有重要意义。该方法是在两种广泛使用的有机金属框架（MIL-53(Al)和 ZIF-8）存在下，通过分散微固相萃取实现的。通过对影响吸收/解吸效率的主要因素进行广泛研究，对萃取步骤进行了优化。通过实验设计获得了萃取和测定空白人体血浆基质中诺龙的最佳条件。在盐浓度为 0.89% (w/v) 的溶液中，2.5 毫克 MIL-53(Al)可在 8.5 分钟内实现对诺龙的最大萃取；而另一种吸附剂 ZIF-8 则需要 10 分钟、4.5 毫克吸附剂和 0.60% (w/v) 的盐浓度才能实现对诺龙的最大萃取。为了找到合适的动力学和等温线模型，还进行了批量实验。最后，研究了所提方法的分析验证。该方法的动态范围为 0.05 至 1 µg/mL。MIL-53(Al)萃取的精密度（以相对标准偏差RSD表示）和准确度（以误差百分比表示）分别为3.90%和3.39%，而ZIF-8萃取的精密度和准确度分别为4.59%和4.50%。该方法成功地测定了加标样品中的诺龙含量，MIL-53(Al)和ZIF-8的回收率分别达到96%和94%。

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来源期刊

Journal of Analytical Chemistry 化学-分析化学

CiteScore

2.10

自引率

9.10%

发文量

146

审稿时长

13 months

期刊介绍： The Journal of Analytical Chemistry is an international peer reviewed journal that covers theoretical and applied aspects of analytical chemistry; it informs the reader about new achievements in analytical methods, instruments and reagents. Ample space is devoted to problems arising in the analysis of vital media such as water and air. Consideration is given to the detection and determination of metal ions, anions, and various organic substances. The journal welcomes manuscripts from all countries in the English or Russian language.