Plastome Analysis of Medicinal Plant Tinospora cordifolia for Species Identification and Authentication

IF 0.6 4区生物学 Q4 GENETICS & HEREDITY Russian Journal of Genetics Pub Date : 2024-07-27 DOI:10.1134/s1022795424700315

J. K. Monpara, K. S. Chudasama, M. L. Vekaria, V. J. Patel, V. S. Thaker

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Abstract

Tinospora cordifolia is an important medicinal plant in ayurvedic system and modern medicine in India and traditionally applied for the treatment of inflammation, allergies and various other ailments. There is no genomic information available for T. cordifolia. In the present study, first time reported complete cp genome of T. cordifolia using high throughput ion torrent genome machine with ion torrent server. The cp genome of T. cordifolia was 150 945 bp in length with GC content of 38.7%, displays a quadripartite structure with two IR regions (IRa: 22 375 bp and IRb: 22 380 bp) that are separated by the LSC region (84 899 bp) and the SSC region (21 291 bp). There are 123 gene, including 85 protein-coding genes, 29 tRNA genes, seven rRNA genes and two pseudogenes (ycf1, rps16). Phylogenetic analysis based on whole cp genome revealed that T. cordifolia is as positioned near to the Menispermum dauricum within the Menispermaceae family. Total 27 SSRs were detected inthe plastomes which include di-, tri-, tetra- penta- and hexanucleotieds repeats. Further, compared with cp genome of other species of Menispermaceae it showed ten unique SSRs. Total twenty-five hypervariable regions loci were found in genome. These data could serve as DNA barcodes for species identification.

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对药用植物天南星进行植物体分析以鉴定和认证物种

摘要 Tinospora cordifolia 是印度阿育吠陀系统和现代医学中的一种重要药用植物，传统上用于治疗炎症、过敏和其他各种疾病。目前还没有关于 T. cordifolia 的基因组信息。本研究利用高通量离子洪流基因组研究机和离子洪流服务器，首次报道了 T. cordifolia 的完整 cp 基因组。T. cordifolia的cp基因组长度为150 945 bp，GC含量为38.7%，呈四方结构，有两个IR区（IRa：22 375 bp和IRb：22 380 bp），被LSC区（84 899 bp）和SSC区（21 291 bp）分开。共有 123 个基因，包括 85 个蛋白质编码基因、29 个 tRNA 基因、7 个 rRNA 基因和两个假基因（ycf1 和 rps16）。基于全 cp 基因组的系统进化分析表明，T. cordifolia 的位置与 Menispermaceae 中的 Menispermum dauricum 相近。质粒中共检测到 27 个 SSR，其中包括二核、三核、四核、五核和六核重复序列。此外，与其他仙人掌科植物的 cp 基因组相比，它显示出 10 个独特的 SSR。在基因组中总共发现了 25 个超变区位点。这些数据可作为物种鉴定的 DNA 条形码。

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来源期刊

Russian Journal of Genetics 生物-遗传学

CiteScore

1.00

自引率

33.30%

发文量

126

审稿时长

1 months

期刊介绍： Russian Journal of Genetics is a journal intended to make significant contribution to the development of genetics. The journal publishes reviews and experimental papers in the areas of theoretical and applied genetics. It presents fundamental research on genetic processes at molecular, cell, organism, and population levels, including problems of the conservation and rational management of genetic resources and the functional genomics, evolutionary genomics and medical genetics.