Expression Profiles of Genes Involved in Lignan Synthesis in Developing Flax Seeds

IF 0.6 4区生物学 Q4 GENETICS & HEREDITY Russian Journal of Genetics Pub Date : 2024-07-27 DOI:10.1134/s1022795424700443

E. N. Pushkova, E. M. Dvorianinova, L. V. Povkhova, T. A. Rozhmina, R. O. Novakovskiy, E. A. Sigova, A. A. Dmitriev, N. V. Melnikova

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Abstract—

Flax seeds are the richest plant source of lignans, which prevent the development of many diseases. In the seeds of cultivated species Linum usitatissimum, the lignan secoisolariciresinol diglucoside (SDG) predominates. In the present study, transcriptome sequencing of flax seeds was performed at five developmental stages for eight varieties differing in lignan content and grown under three variants of conditions, and expression of the PLR1 and UGT74S1 genes, which play a key role in the SDG synthesis, was assessed. Co-expression of the PLR1 and UGT74S1 genes, as well as tenfold and hundredfold changes in the expression level of these genes during seed development were revealed, which testified to their role in the synthesis of SDG in flax seeds. Reduced temperature (16°C) and excessive watering led to a shift in the maximum expression level of both genes to a later date (14th day after flowering), compared to the conditions of insufficient watering and elevated temperature (24°C), as well as optimum conditions (20°C) (7th day after flowering). Moreover, at elevated temperatures and insufficient watering, the PLR1 and UGT74S1 expression levels were lower than under optimum conditions. No association between the lignan content in seeds of the studied flax varieties and the PLR1 and UGT74S1 expression levels was found. These findings provide insight into the contribution of genotype and environment to the expression of key genes for SDG synthesis, which, among other things, is necessary for the development of optimum approaches for obtaining the lignan-rich flax seeds.

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发育中亚麻种子中参与木质素合成的基因的表达图谱

摘要--亚麻种子是木酚素最丰富的植物来源，它能预防多种疾病的发生。在栽培品种亚麻（Linum usitatissimum）的种子中，主要是木质素仲异落叶松脂素二葡萄糖苷（SDG）。在本研究中，对在三种不同条件下生长的八个木质素含量不同的品种的亚麻种子的五个发育阶段进行了转录组测序，并评估了在 SDG 合成中起关键作用的 PLR1 和 UGT74S1 基因的表达情况。结果表明，PLR1 和 UGT74S1 基因的共表达，以及在种子发育过程中这些基因表达水平的十倍和百倍变化，证明了它们在亚麻种子中合成 SDG 的作用。与浇水不足和温度升高（24°C）的条件以及最佳条件（20°C）（开花后第 7 天）相比，温度降低（16°C）和浇水过多会导致这两个基因的最大表达水平推迟（开花后第 14 天）。此外，在温度升高和浇水不足的条件下，PLR1 和 UGT74S1 的表达水平低于最适条件。所研究亚麻品种种子中的木质素含量与 PLR1 和 UGT74S1 的表达水平之间没有关联。这些发现让人们深入了解了基因型和环境对合成 SDG 的关键基因表达的影响，这对于开发获取富含木质素的亚麻种子的最佳方法是非常必要的。

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来源期刊

Russian Journal of Genetics 生物-遗传学

CiteScore

1.00

自引率

33.30%

发文量

126

审稿时长

1 months

期刊介绍： Russian Journal of Genetics is a journal intended to make significant contribution to the development of genetics. The journal publishes reviews and experimental papers in the areas of theoretical and applied genetics. It presents fundamental research on genetic processes at molecular, cell, organism, and population levels, including problems of the conservation and rational management of genetic resources and the functional genomics, evolutionary genomics and medical genetics.