{"title":"Phylogeography of apomictic and outcrossing individuals in invasive and native populations of Ardisia crenata (Primulaceae)","authors":"Wataru Noyori, Yuji Isagi, Naoto Nakamura, Shuichiro Tagane, Gerardo Celis, Kaoru Kitajima","doi":"10.1111/1442-1984.12482","DOIUrl":null,"url":null,"abstract":"Phylogeography of invasive exotic plant species is crucial for identifying the source population from which a given invading population has originated. <jats:italic>Ardisia crenata</jats:italic> Sims, a shade tolerant shrub native to East Asia, is one of the most serious invasive exotic plants in Florida, North America. The objective of this study is to investigate the genetic differentiation and phylogeographical relationships among <jats:italic>A</jats:italic>. <jats:italic>crenata</jats:italic> native populations in Japan and invasive populations in Florida. We analyzed DNA from 188 individuals gathered from eight populations in Japan and four subpopulations in Florida using double‐digestion restriction‐associated DNA sequencing (ddRAD‐seq) and estimated a maximum likelihood phylogenetic tree among them. The results revealed four genetic clusters, with a striking dominance of genetically identical clonal individuals within the invading populations in Florida and four Honshu populations in Japan. These groups were genetically close to each other and to one cultivated individual sampled in Japan. In contrast, the individuals from Miyazaki, Yakushima, Kochi, and Iriomote in southwestern Japan were genetically distinct from Honshu and Florida individuals, showing high genetic diversity indicative of outcrossing. Given that <jats:italic>A. crenata</jats:italic> does not exhibit a vegetative clonal habit and all wild and cultivated plants regenerate from seeds, these results suggest that Honshu and Florida populations exclusively reproduced with seeds produced by apomixis.","PeriodicalId":54601,"journal":{"name":"Plant Species Biology","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Species Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/1442-1984.12482","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ECOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Phylogeography of invasive exotic plant species is crucial for identifying the source population from which a given invading population has originated. Ardisia crenata Sims, a shade tolerant shrub native to East Asia, is one of the most serious invasive exotic plants in Florida, North America. The objective of this study is to investigate the genetic differentiation and phylogeographical relationships among A. crenata native populations in Japan and invasive populations in Florida. We analyzed DNA from 188 individuals gathered from eight populations in Japan and four subpopulations in Florida using double‐digestion restriction‐associated DNA sequencing (ddRAD‐seq) and estimated a maximum likelihood phylogenetic tree among them. The results revealed four genetic clusters, with a striking dominance of genetically identical clonal individuals within the invading populations in Florida and four Honshu populations in Japan. These groups were genetically close to each other and to one cultivated individual sampled in Japan. In contrast, the individuals from Miyazaki, Yakushima, Kochi, and Iriomote in southwestern Japan were genetically distinct from Honshu and Florida individuals, showing high genetic diversity indicative of outcrossing. Given that A. crenata does not exhibit a vegetative clonal habit and all wild and cultivated plants regenerate from seeds, these results suggest that Honshu and Florida populations exclusively reproduced with seeds produced by apomixis.
外来入侵植物物种的系统地理学对于确定特定入侵种群的来源种群至关重要。Ardisia crenata Sims 是一种原产于东亚的耐阴灌木,是北美佛罗里达州最严重的外来入侵植物之一。本研究的目的是调查 A. crenata 在日本的原生种群与佛罗里达州入侵种群之间的遗传分化和系统地理学关系。我们使用双消化限制性相关 DNA 测序(ddRAD-seq)分析了从日本八个种群和佛罗里达四个亚种群收集的 188 个个体的 DNA,并估计了它们之间的最大似然系统发生树。结果显示,在佛罗里达州的入侵种群和日本本州的四个种群中,有四个基因群,其中基因相同的克隆个体占显著优势。这些群体在基因上相互接近,并与在日本采样的一个栽培个体接近。相比之下,来自日本西南部宫崎、屋久岛、高知和西表的个体在基因上与本州和佛罗里达的个体截然不同,显示出高度的遗传多样性,表明存在外交。鉴于 A. crenata 并不表现出无性繁殖的习性,而且所有野生和栽培植物都是从种子再生的,这些结果表明本州和佛罗里达的种群完全是通过无性繁殖产生的种子进行繁殖的。
期刊介绍:
Plant Species Biology is published four times a year by The Society for the Study of Species Biology. Plant Species Biology publishes research manuscripts in the fields of population biology, pollination biology, evolutionary ecology, biosystematics, co-evolution, and any other related fields in biology. In addition to full length papers, the journal also includes short research papers as notes and comments. Invited articles may be accepted or occasion at the request of the Editorial Board. Manuscripts should contain new results of empirical and/or theoretical investigations concerning facts, processes, mechanisms or concepts of evolutionary as well as biological phenomena. Papers that are purely descriptive are not suitable for this journal. Notes & comments of the following contents will not be accepted for publication: Development of DNA markers. The journal is introducing ''Life history monographs of Japanese plant species''. The journal is dedicated to minimizing the time between submission, review and publication and to providing a high quality forum for original research in Plant Species Biology.