Copper doped hydroxyapatite nanocomposite thin films: synthesis, physico-chemical and biological evaluation.

IF 4.1 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Biometals Pub Date : 2024-07-29 DOI:10.1007/s10534-024-00620-2
Carmen Steluta Ciobanu, Daniela Predoi, Simona Liliana Iconaru, Mihai Valentin Predoi, Liliana Ghegoiu, Nicolas Buton, Mikael Motelica-Heino
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Abstract

Cu-doped hydroxyapatite (CuHAp) thin films were obtained using spin coating method. To make these thin films, CuHAp suspensions obtained by sol-gel method were used. The coatings obtained were thermally treated at 500 °C. After the thermal treatment, the thin films were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM). Moreover, the stability of the suspensions before being used to obtain the thin films was certified by dynamic light scattering (DLS), zeta potential methods and ultrasound measurements. In the XRD patterns, the peaks associated with hexagonal hydroxyapatite were identified in accordance with JCPDS no. 09-0432. EDS and XPS results confirmed the presence of Cu ions in the samples. Data about the morphological features and chemical composition of CuHAp thin films were obtained by performing scanning electron microscopy (SEM) measurements. Our results suggest that the CuHAp thin films surface is continuous and homogenous. The presence of the functional groups in the CuHAp thin films was confirmed by Fourier-transform infrared spectroscopy (FTIR) and Raman spectroscopy studies. Information about the surface topography of the CuHAp thin films has been obtained using atomic force microscopy (AFM). The AFM images determined that the surface topography of the CuHAp thin layer is homogenous and continuous without presenting any unevenness or fissures. The cytotoxicity of CuHAp thin films was assessed using human gingival fibroblasts (HGF-1) cells. The results of the cell viability assays demonstrated that the thin films presented good biocompatible properties towards the HGF-1 cells. Additionally, the adherence and development of HGF-1 cells on the surface of CuHAp thin films were determined using AFM. The AFM surface topographies highlighted that the CuHAp thin film's surface favored the attachment and proliferation of HGF-1 cells on their surface.

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掺铜羟基磷灰石纳米复合薄膜:合成、物理化学和生物学评价。
采用旋涂法获得了掺铜羟基磷灰石(CuHAp)薄膜。为了制作这些薄膜,使用了通过溶胶-凝胶法获得的 CuHAp 悬浮液。获得的涂层在 500 °C 下进行热处理。热处理后,用 X 射线衍射(XRD)和扫描电子显微镜(SEM)对薄膜进行了表征。此外,还通过动态光散射(DLS)、zeta 电位法和超声波测量来验证用于获得薄膜前悬浮液的稳定性。在 XRD 图谱中,根据 JCPDS 编号 09-0432 确定了与六方羟基磷灰石相关的峰值。EDS 和 XPS 结果证实了样品中存在铜离子。通过扫描电子显微镜(SEM)测量获得了 CuHAp 薄膜的形态特征和化学成分数据。我们的研究结果表明,CuHAp 薄膜表面是连续和均匀的。傅立叶变换红外光谱(FTIR)和拉曼光谱研究证实了 CuHAp 薄膜中官能团的存在。使用原子力显微镜(AFM)获得了有关 CuHAp 薄膜表面形貌的信息。原子力显微镜图像显示,CuHAp 薄膜的表面形貌均匀连续,没有任何凹凸或裂缝。使用人牙龈成纤维细胞(HGF-1)评估了 CuHAp 薄膜的细胞毒性。细胞存活率测定结果表明,薄膜对 HGF-1 细胞具有良好的生物相容性。此外,还使用原子力显微镜测定了 HGF-1 细胞在 CuHAp 薄膜表面的附着和发育情况。原子力显微镜表面形貌显示,CuHAp 薄膜表面有利于 HGF-1 细胞在其表面附着和增殖。
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来源期刊
Biometals
Biometals 生物-生化与分子生物学
CiteScore
5.90
自引率
8.60%
发文量
111
审稿时长
3 months
期刊介绍: BioMetals is the only established journal to feature the important role of metal ions in chemistry, biology, biochemistry, environmental science, and medicine. BioMetals is an international, multidisciplinary journal singularly devoted to the rapid publication of the fundamental advances of both basic and applied research in this field. BioMetals offers a forum for innovative research and clinical results on the structure and function of: - metal ions - metal chelates, - siderophores, - metal-containing proteins - biominerals in all biosystems. - BioMetals rapidly publishes original articles and reviews. BioMetals is a journal for metals researchers who practice in medicine, biochemistry, pharmacology, toxicology, microbiology, cell biology, chemistry, and plant physiology who are based academic, industrial and government laboratories.
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