Non-sterile cultivation of Yarrowia lipolytica in fed-batch mode for the production of lipids and biomass.

IF 2.5 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology Progress Pub Date : 2024-07-28 DOI:10.1002/btpr.3498
Atith V Chitnis, Abhishek S Dhoble
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Abstract

A reduction in the cost of production and energy requirement is necessary for developing sustainable commercial bioprocesses. Bypassing sterilization, which is an energy and cost-intensive part of bioprocesses could be a way to achieve this. In this study, nonsterile cultivation of Yarrowia lipolytica was done on a synthetic medium containing acetic acid as the sole carbon source using two different strategies in the fed-batch mode. The contamination percentages throughout the process were measured using flow cytometry and complemented using brightfield microscopy. Maximum biomass and lipid yields of 0.57 (g biomass/g substrate) and 0.17 (g lipids/g substrate), respectively, and maximum biomass and lipid productivities of 0.085 and 0.023 g/L/h, respectively, were obtained in different fed-batch strategies. Feeding at the point of stationary phase resulted in better biomass yield and productivity with less than 2% contamination till 48 h. Feeding to maintain a minimum acetic level resulted in better lipid yield and productivity with less than 2% contamination during the complete process. The results of this study demonstrate the potential for cultivating Y. lipolytica in nonsterile conditions and monitoring the contamination throughout the process using flow cytometry.

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以饲料批处理模式对脂肪溶解亚罗菌进行非灭菌培养,以生产脂类和生物质。
要开发可持续的商业生物工艺,就必须降低生产成本和能源需求。绕过生物工艺中能源和成本密集型的灭菌环节可能是实现这一目标的途径之一。在本研究中,采用两种不同的策略,在含有醋酸作为唯一碳源的合成培养基上,以喂料批处理模式对脂肪分解亚罗菌进行了无菌培养。使用流式细胞仪测量了整个过程中的污染率,并使用明视野显微镜进行了补充。在不同的喂料批处理策略下,生物质和脂质的最高产量分别为 0.57(克生物质/克底物)和 0.17(克脂质/克底物),生物质和脂质的最高生产率分别为 0.085 和 0.023 克/升/小时。在静止期投料可获得更好的生物质产量和生产率,48 小时内的污染率低于 2%;投料以维持最低乙酸水平可获得更好的脂质产量和生产率,整个过程中的污染率低于 2%。这项研究的结果证明了在非无菌条件下培养脂溶性酵母菌并使用流式细胞仪监测整个过程中污染情况的潜力。
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来源期刊
Biotechnology Progress
Biotechnology Progress 工程技术-生物工程与应用微生物
CiteScore
6.50
自引率
3.40%
发文量
83
审稿时长
4 months
期刊介绍: Biotechnology Progress , an official, bimonthly publication of the American Institute of Chemical Engineers and its technological community, the Society for Biological Engineering, features peer-reviewed research articles, reviews, and descriptions of emerging techniques for the development and design of new processes, products, and devices for the biotechnology, biopharmaceutical and bioprocess industries. Widespread interest includes application of biological and engineering principles in fields such as applied cellular physiology and metabolic engineering, biocatalysis and bioreactor design, bioseparations and downstream processing, cell culture and tissue engineering, biosensors and process control, bioinformatics and systems biology, biomaterials and artificial organs, stem cell biology and genetics, and plant biology and food science. Manuscripts concerning the design of related processes, products, or devices are also encouraged. Four types of manuscripts are printed in the Journal: Research Papers, Topical or Review Papers, Letters to the Editor, and R & D Notes.
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