Antimicrobial and anthelmintic effects of copper nanoparticles against Koi carp parasites and their toxicity

IF 2.2 3区 农林科学 Q2 FISHERIES Journal of fish diseases Pub Date : 2024-07-29 DOI:10.1111/jfd.14003
Gracienhe Gomes dos Santos, Liseth Vanessa Perengue Riofrio, Ana Paula Sousa, Paola Capistrano Santos, Alexandre Vaz Silva, Emilly Monteiro Lopes, Domickson Silva Costa, Jucimauro de Araújo Pereira Júnior, Giulia Beatrice Ferreira, Graziella Vivine Gonçalves de Matos Silva, Marilia Tedesco, Diego José Nogueira, Gabriela Tomas Jerônimo, Mauricio Laterça Martins
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Abstract

This study investigated the in vitro antimicrobial and anthelmintic effect of copper nanoparticles (CuNPs) against the bacterium Aeromonas hydrophila, the monogeneans Dactylogyrus minutus, Dactylogyrus extensus, Gyrodactylus cyprini, and the cestode Schyzocotyle acheilognathi, as well as their toxicity to Cyprinus carpio Koi. In the antimicrobial in vitro test, the inhibition zone method and minimum inhibitory concentration (MIC) were performed. In order to determine the time and efficacy of monogenean parasite mortality, the parasites were exposed to CuNP concentrations of 20, 50, 100, 150, 200, and 300 mg L−1, and a control group with tank water and one with copper sulphate pentahydrate (CuSO4.5H2O) at a concentration of 0.3 mg L−1, performed in triplicate. The parasites were observed every 10 min for 300 min, and mortality was recorded. For the cestodes, parasites were immersed in CuNP concentrations of 50, 100, 150, and 300 mg L−1. At the end of the in vitro tests, the anthelmintic efficacy of each treatment was calculated. To assess the tolerance and toxicity in fish, they were exposed to CuNP concentrations of 0.6, 1.25, 2.5, 5, 10, 20, and 50 mg L−1 for 12 h. The MIC demonstrated that CuNPs effectively inhibited the growth of A. hydrophila up to a dilution of 12,500 mg L−1 and showed an inhibition zone of 14.0 ± 1.6 mm for CuNPs. The results of anthelmintic activity showed a dose-dependent effect of concentration for both groups of parasites, with the most effective concentration being 300 mg L−1 in 120 min. In the toxicity test, the carps showed tolerance to lower concentrations. The study indicated that CuNPs were effective against the studied pathogens. However, it proved to be toxic to fish at high concentrations. The use of low concentrations is recommended still requires further investigation.

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纳米铜颗粒对锦鲤寄生虫的抗菌和驱虫作用及其毒性。
本研究探讨了纳米铜粒子(CuNPs)对嗜水气单胞菌(Aeromonas hydrophila)、单胞菌(Dactylogyrus minutus、Dactylogyrus extensus、Gyrodactylus cyprini)和绦虫(Schyzocotyle acheilognathi)的体外抗菌和驱虫效果,以及对鲤科锦鲤的毒性。抗菌体外试验采用抑菌区法和最低抑菌浓度法。为了确定单基因寄生虫死亡的时间和效果,寄生虫分别接触了浓度为 20、50、100、150、200 和 300 毫克/升-1 的 CuNP,以及浓度为 0.3 毫克/升-1 的水箱水和五水硫酸铜(CuSO4.5H2O)的对照组,一式三份。每隔 10 分钟观察寄生虫一次,持续 300 分钟,并记录死亡率。对于绦虫,将寄生虫浸入浓度为 50、100、150 和 300 mg L-1 的 CuNP 中。体外试验结束后,计算每种处理的驱虫效果。为了评估鱼类的耐受性和毒性,将鱼类暴露于 0.6、1.25、2.5、5、10、20 和 50 mg L-1 浓度的 CuNPs 中 12 小时。MIC 显示,CuNPs 在 12,500 mg L-1 稀释度范围内可有效抑制蚜虫的生长,CuNPs 的抑制区为 14.0 ± 1.6 mm。对两组寄生虫的抗蠕虫活性结果表明,浓度具有剂量依赖性效应,120 分钟内最有效的浓度为 300 mg L-1。在毒性试验中,鲤鱼对较低的浓度表现出耐受性。研究表明,CuNPs 对所研究的病原体有效。不过,高浓度时对鱼类有毒。建议使用低浓度仍需进一步研究。
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来源期刊
Journal of fish diseases
Journal of fish diseases 农林科学-海洋与淡水生物学
CiteScore
4.60
自引率
12.00%
发文量
170
审稿时长
6 months
期刊介绍: Journal of Fish Diseases enjoys an international reputation as the medium for the exchange of information on original research into all aspects of disease in both wild and cultured fish and shellfish. Areas of interest regularly covered by the journal include: -host-pathogen relationships- studies of fish pathogens- pathophysiology- diagnostic methods- therapy- epidemiology- descriptions of new diseases
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