Gracienhe Gomes dos Santos, Liseth Vanessa Perengue Riofrio, Ana Paula Sousa, Paola Capistrano Santos, Alexandre Vaz Silva, Emilly Monteiro Lopes, Domickson Silva Costa, Jucimauro de Araújo Pereira Júnior, Giulia Beatrice Ferreira, Graziella Vivine Gonçalves de Matos Silva, Marilia Tedesco, Diego José Nogueira, Gabriela Tomas Jerônimo, Mauricio Laterça Martins
{"title":"Antimicrobial and anthelmintic effects of copper nanoparticles against Koi carp parasites and their toxicity","authors":"Gracienhe Gomes dos Santos, Liseth Vanessa Perengue Riofrio, Ana Paula Sousa, Paola Capistrano Santos, Alexandre Vaz Silva, Emilly Monteiro Lopes, Domickson Silva Costa, Jucimauro de Araújo Pereira Júnior, Giulia Beatrice Ferreira, Graziella Vivine Gonçalves de Matos Silva, Marilia Tedesco, Diego José Nogueira, Gabriela Tomas Jerônimo, Mauricio Laterça Martins","doi":"10.1111/jfd.14003","DOIUrl":null,"url":null,"abstract":"<p>This study investigated the in vitro antimicrobial and anthelmintic effect of copper nanoparticles (CuNPs) against the bacterium <i>Aeromonas hydrophila</i>, the monogeneans <i>Dactylogyrus minutus</i>, <i>Dactylogyrus extensus</i>, <i>Gyrodactylus cyprini</i>, and the cestode <i>Schyzocotyle acheilognathi</i>, as well as their toxicity to <i>Cyprinus carpio</i> Koi. In the antimicrobial in vitro test, the inhibition zone method and minimum inhibitory concentration (MIC) were performed. In order to determine the time and efficacy of monogenean parasite mortality, the parasites were exposed to CuNP concentrations of 20, 50, 100, 150, 200, and 300 mg L<sup>−1</sup>, and a control group with tank water and one with copper sulphate pentahydrate (CuSO<sub>4</sub>.<sub>5</sub>H<sub>2</sub>O) at a concentration of 0.3 mg L<sup>−1</sup>, performed in triplicate. The parasites were observed every 10 min for 300 min, and mortality was recorded. For the cestodes, parasites were immersed in CuNP concentrations of 50, 100, 150, and 300 mg L<sup>−1</sup>. At the end of the in vitro tests, the anthelmintic efficacy of each treatment was calculated. To assess the tolerance and toxicity in fish, they were exposed to CuNP concentrations of 0.6, 1.25, 2.5, 5, 10, 20, and 50 mg L<sup>−1</sup> for 12 h. The MIC demonstrated that CuNPs effectively inhibited the growth of <i>A</i>. <i>hydrophila</i> up to a dilution of 12,500 mg L<sup>−1</sup> and showed an inhibition zone of 14.0 ± 1.6 mm for CuNPs. The results of anthelmintic activity showed a dose-dependent effect of concentration for both groups of parasites, with the most effective concentration being 300 mg L<sup>−1</sup> in 120 min. In the toxicity test, the carps showed tolerance to lower concentrations. The study indicated that CuNPs were effective against the studied pathogens. However, it proved to be toxic to fish at high concentrations. The use of low concentrations is recommended still requires further investigation.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":"47 11","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of fish diseases","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jfd.14003","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FISHERIES","Score":null,"Total":0}
引用次数: 0
Abstract
This study investigated the in vitro antimicrobial and anthelmintic effect of copper nanoparticles (CuNPs) against the bacterium Aeromonas hydrophila, the monogeneans Dactylogyrus minutus, Dactylogyrus extensus, Gyrodactylus cyprini, and the cestode Schyzocotyle acheilognathi, as well as their toxicity to Cyprinus carpio Koi. In the antimicrobial in vitro test, the inhibition zone method and minimum inhibitory concentration (MIC) were performed. In order to determine the time and efficacy of monogenean parasite mortality, the parasites were exposed to CuNP concentrations of 20, 50, 100, 150, 200, and 300 mg L−1, and a control group with tank water and one with copper sulphate pentahydrate (CuSO4.5H2O) at a concentration of 0.3 mg L−1, performed in triplicate. The parasites were observed every 10 min for 300 min, and mortality was recorded. For the cestodes, parasites were immersed in CuNP concentrations of 50, 100, 150, and 300 mg L−1. At the end of the in vitro tests, the anthelmintic efficacy of each treatment was calculated. To assess the tolerance and toxicity in fish, they were exposed to CuNP concentrations of 0.6, 1.25, 2.5, 5, 10, 20, and 50 mg L−1 for 12 h. The MIC demonstrated that CuNPs effectively inhibited the growth of A. hydrophila up to a dilution of 12,500 mg L−1 and showed an inhibition zone of 14.0 ± 1.6 mm for CuNPs. The results of anthelmintic activity showed a dose-dependent effect of concentration for both groups of parasites, with the most effective concentration being 300 mg L−1 in 120 min. In the toxicity test, the carps showed tolerance to lower concentrations. The study indicated that CuNPs were effective against the studied pathogens. However, it proved to be toxic to fish at high concentrations. The use of low concentrations is recommended still requires further investigation.
期刊介绍:
Journal of Fish Diseases enjoys an international reputation as the medium for the exchange of information on original research into all aspects of disease in both wild and cultured fish and shellfish. Areas of interest regularly covered by the journal include:
-host-pathogen relationships-
studies of fish pathogens-
pathophysiology-
diagnostic methods-
therapy-
epidemiology-
descriptions of new diseases