The cytoskeleton controls the dynamics of plasma membrane proteins and facilitates their endocytosis in plants.

IF 6.5 1区 生物学 Q1 PLANT SCIENCES Plant Physiology Pub Date : 2024-11-04 DOI:10.1093/plphys/kiae403
Pengyun Luo, Xinxiu Zuo, Yufen Bu, Hongping Qian, Changwen Xu, Shihui Niu, Jinxing Lin, Yaning Cui
{"title":"The cytoskeleton controls the dynamics of plasma membrane proteins and facilitates their endocytosis in plants.","authors":"Pengyun Luo, Xinxiu Zuo, Yufen Bu, Hongping Qian, Changwen Xu, Shihui Niu, Jinxing Lin, Yaning Cui","doi":"10.1093/plphys/kiae403","DOIUrl":null,"url":null,"abstract":"<p><p>Plasma membranes (PMs) are highly dynamic structures where lipids and proteins can theoretically diffuse freely. However, reports indicate that PM proteins do not freely diffuse within their planes but are constrained by cytoskeleton networks, though the mechanisms for how the cytoskeleton restricts lateral diffusion of plant PM proteins are unclear. Through single-molecule tracking, we investigated the dynamics of 6 Arabidopsis (Arabidopsis thaliana) PM proteins with diverse structures and found distinctions in sizes and dynamics among these proteins. Moreover, we showed that the cytoskeleton, particularly microtubules, limits the diffusion of PM proteins, including transmembrane and membrane-anchoring proteins. Interestingly, the microfilament skeleton regulates intracellular transport of endocytic cargo. Therefore, these findings indicate that the cytoskeleton controls signal transduction by limiting diffusion of PM proteins in specific membrane compartments and participating in transport of internalized cargo vesicles, thus actively regulating plant signal transduction.</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":"1813-1825"},"PeriodicalIF":6.5000,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1093/plphys/kiae403","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Plasma membranes (PMs) are highly dynamic structures where lipids and proteins can theoretically diffuse freely. However, reports indicate that PM proteins do not freely diffuse within their planes but are constrained by cytoskeleton networks, though the mechanisms for how the cytoskeleton restricts lateral diffusion of plant PM proteins are unclear. Through single-molecule tracking, we investigated the dynamics of 6 Arabidopsis (Arabidopsis thaliana) PM proteins with diverse structures and found distinctions in sizes and dynamics among these proteins. Moreover, we showed that the cytoskeleton, particularly microtubules, limits the diffusion of PM proteins, including transmembrane and membrane-anchoring proteins. Interestingly, the microfilament skeleton regulates intracellular transport of endocytic cargo. Therefore, these findings indicate that the cytoskeleton controls signal transduction by limiting diffusion of PM proteins in specific membrane compartments and participating in transport of internalized cargo vesicles, thus actively regulating plant signal transduction.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
在植物体内,细胞骨架控制着质膜蛋白的动态,并促进它们的内吞。
质膜(PM)是一种高度动态的结构,理论上脂质和蛋白质可以在其中自由扩散。然而,有报告表明,质膜蛋白并不能在其平面内自由扩散,而是受到细胞骨架网络的限制,但细胞骨架如何限制植物质膜蛋白横向扩散的机制尚不清楚。通过单分子追踪,我们研究了拟南芥(Arabidopsis thaliana)六种具有不同结构的 PM 蛋白的动态,发现了这些蛋白在大小和动态方面的差异。此外,我们还发现细胞骨架,尤其是微管,限制了包括跨膜和膜锚定蛋白在内的 PM 蛋白的扩散。有趣的是,微丝骨架能调节内细胞货物的胞内运输。因此,这些研究结果表明,细胞骨架通过限制PM蛋白在特定膜区的扩散和参与内化货物囊泡的运输来控制信号转导,从而积极调节植物的信号转导。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Plant Physiology
Plant Physiology 生物-植物科学
CiteScore
12.20
自引率
5.40%
发文量
535
审稿时长
2.3 months
期刊介绍: Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research. As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.
期刊最新文献
The receptor MIK2 interacts with the kinase RKS1 to control quantitative disease resistance to Xanthomonas campestris The RALF1-FERONIA complex phosphorylates FREE1 to attenuate abscisic acid signaling during seedling establishment Follow the calcium road: Conserved mechanisms of growth and development in Marchantia polymorpha. GmERFVII transcription factors upregulate PATHOGENESIS-RELATED10 and contribute to soybean cyst nematode resistance. Go with the flux: Modelling accurately predicts phenotypes of Arabidopsis lipid mutants.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1