Prevalence and susceptibility of microorganisms in vaginal isolates

Abstract

Microbiological analysis of genital secretion allows isolate and identify the microorganisms responsible for infection in the vaginal canal. The objective of this research was to isolate and identify the microorganisms that cause vaginal infection and analyze antimicrobial susceptibility. 210 samples from women with a clinical diagnosis of vaginal infection who attended the microbiology service were analyzed. Microbial analysis, Gram staining was performed, and they were seeded in specific culture media, as well as in Mueller-Hinton for sensitivity tests. 115 microorganisms were identified, 57% of the isolated correspond to bacterial species, the most frequent was Gardnerella vaginalis with 38.5%. 43% of the isolated were yeasts, the predominant species was Candida albicans with 78%. On the other hand, testing for susceptibility to various antibiotics, Gardnerella vaginalis showed 100% resistance to gentamicin and netilmicin. Ampicillin, norfloxacin and sulfamethoxazole/trimethoprim showed sensitivity greater than 80%. In antifungal susceptibility tests, Candida albicans showed 100% resistance to nystatin and 90 to 100% sensitivity to fluconazole and ketoconazole, respectively. Of the isolated species, Gardnerella vaginalis and Candida albicans were the most frequent in vaginal infections; in addition, both microorganisms presented a variable susceptibility