The Role of Erythropoietin in Bovine Sperm Physiology

IF 2.7 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Animals Pub Date : 2024-07-26 DOI:10.3390/ani14152175

V. Sapanidou, Byron Asimakopoulos, Theodoros Lialiaris, Sophia N. Lavrentiadou, Kostantinos Feidantsis, Georgios Kourousekos, M. Tsantarliotou

{"title":"The Role of Erythropoietin in Bovine Sperm Physiology","authors":"V. Sapanidou, Byron Asimakopoulos, Theodoros Lialiaris, Sophia N. Lavrentiadou, Kostantinos Feidantsis, Georgios Kourousekos, M. Tsantarliotou","doi":"10.3390/ani14152175","DOIUrl":null,"url":null,"abstract":"Erythropoietin (EPO), a hormone secreted mainly by the kidney, exerts its biological function by binding to its cell-surface receptor (EpoR). The presence of EPO and EpoR in the male and female reproductive system has been verified. Therefore, some of the key properties of EPO, such as its antioxidant and antiapoptotic effects, could improve the fertilizing capacity of spermatozoa. In the present study, the effect of two different concentrations of EPO (10 mIU/μL and 100 mIU/μL) on bovine sperm-quality parameters was evaluated during a post-thawing 4-h incubation at 37 °C. EPO had a positive effect on sperm motility, viability, and total antioxidant capacity. Moreover, EPO inhibited apoptosis, as it reduced both BCL2-associated X apoptosis regulator (Bax)/B-cell lymphoma 2 (Bcl-2) ratio and cleaved cysteine-aspartic proteases (caspases) substrate levels in a dose-dependent manner. In addition, EPO induced sperm capacitation and acrosome reaction in spermatozoa incubated in capacitation conditioned medeia. These results establish a foundation for the physiological role of EPO in reproductive processes and hopefully will provide an incentive for further research in order to fully decipher the role of EPO in sperm physiology and reproduction.","PeriodicalId":7955,"journal":{"name":"Animals","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animals","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3390/ani14152175","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}

引用次数: 0

Abstract

Erythropoietin (EPO), a hormone secreted mainly by the kidney, exerts its biological function by binding to its cell-surface receptor (EpoR). The presence of EPO and EpoR in the male and female reproductive system has been verified. Therefore, some of the key properties of EPO, such as its antioxidant and antiapoptotic effects, could improve the fertilizing capacity of spermatozoa. In the present study, the effect of two different concentrations of EPO (10 mIU/μL and 100 mIU/μL) on bovine sperm-quality parameters was evaluated during a post-thawing 4-h incubation at 37 °C. EPO had a positive effect on sperm motility, viability, and total antioxidant capacity. Moreover, EPO inhibited apoptosis, as it reduced both BCL2-associated X apoptosis regulator (Bax)/B-cell lymphoma 2 (Bcl-2) ratio and cleaved cysteine-aspartic proteases (caspases) substrate levels in a dose-dependent manner. In addition, EPO induced sperm capacitation and acrosome reaction in spermatozoa incubated in capacitation conditioned medeia. These results establish a foundation for the physiological role of EPO in reproductive processes and hopefully will provide an incentive for further research in order to fully decipher the role of EPO in sperm physiology and reproduction.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

促红细胞生成素在牛精子生理学中的作用

促红细胞生成素（EPO）是一种主要由肾脏分泌的激素，通过与其细胞表面受体（EpoR）结合发挥生物功能。EPO 和 EpoR 在男性和女性生殖系统中的存在已得到证实。因此，EPO 的一些关键特性，如抗氧化和抗凋亡作用，可提高精子的受精能力。本研究评估了两种不同浓度的 EPO（10 mIU/μL 和 100 mIU/μL）对牛精子质量参数的影响。EPO 对精子活力、存活率和总抗氧化能力有积极影响。此外，EPO还能抑制细胞凋亡，因为它能以剂量依赖的方式降低BCL2相关X细胞凋亡调节因子（Bax）/B细胞淋巴瘤2（Bcl-2）比率和半胱氨酸-天冬氨酸蛋白酶（caspases）底物的裂解水平。此外，EPO 还能诱导精子获能和在获能条件介质中培养的精子发生顶体反应。这些结果为 EPO 在生殖过程中的生理作用奠定了基础，希望能为进一步的研究提供动力，以全面解读 EPO 在精子生理和生殖过程中的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Animals Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

4.90

自引率

16.70%

发文量

3015

审稿时长

20.52 days

期刊介绍： Animals (ISSN 2076-2615) is an international and interdisciplinary scholarly open access journal. It publishes original research articles, reviews, communications, and short notes that are relevant to any field of study that involves animals, including zoology, ethnozoology, animal science, animal ethics and animal welfare. However, preference will be given to those articles that provide an understanding of animals within a larger context (i.e., the animals'' interactions with the outside world, including humans). There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental details and/or method of study, must be provided for research articles. Articles submitted that involve subjecting animals to unnecessary pain or suffering will not be accepted, and all articles must be submitted with the necessary ethical approval (please refer to the Ethical Guidelines for more information).