Animals最新文献

Locusta migratoria manilensis (Meyen) is a highly destructive insect pest worldwide. However, excessive reliance on insecticides has resulted in significant environmental pollution. Biocontrol complexes combine two or more BCAs to address the limitations of individual agents. However, biocontrol complex for locust control has been rarely reported. Here, we propose BioControl 3.0, which integrates Metarhizium anisopliae var. acridum (Driver and Milner) and Carabus smaragdinus (Fischer von Waldheim) for locust control. We evaluated this system through a series of laboratory bioassays and semi-field cage experiments, comparing single-agent applications, sequential combinations (BioControl 2.0), and predator-mediated delivery (BioControl 3.0), and quantified locust mortality and interaction effects between predation and infection We found that M. anisopliae caused >85% mortality of locust nymphs at 1 × 10⁸ conidia/mL (LT₅₀ ≈ 6 days) while exhibiting negligible virulence toward C. smaragdinus. BioControl 2.0 (sequential application) increased mortality compared to single agents. However, this approach revealed a significant negative interaction between predation and infection, which limited the total control efficacy. BioControl 3.0 (predator-vectored fungus) achieved the highest corrected mortality, with predation and infection acting independently and additively (no detectable antagonistic interaction). By leveraging a predatory vector, BioControl 3.0 decouples negative interaction and harnesses dual biotic pressures, offering a cost-effective, environmentally benign alternative to conventional locust control. Our findings provide a blueprint for designing integrated predator-pathogen complexes and optimizing deployment strategies for sustainable management of locust outbreaks.

Background: Dental and respiratory diseases are common in companion rabbits. While upper respiratory tract disorders have been described in association with dental pathology, the possible correlation between dental disease and pulmonary lesions has not yet been investigated. Methods: A cross-sectional study was conducted on client-owned rabbits presented for suspected dental disease that underwent head and thoracic computed tomography (2012-2025) at a veterinary teaching hospital. Seventy-three rabbits met the inclusion criteria (mean age 4.96 years). Results: Dental involvement was present in 81% (59/73) of the rabbits and pulmonary lesions in 29% (21/73). Among rabbits with dental disease, frequent osseous findings included osteomyelitis (49%), inflammatory resorption (42%), and maxillary deformities (33%). Pulmonary abnormalities occurred in 32% of rabbits with dental disease compared to 14% of those without. In Poisson models with log link adjusted for age and sex, dental disease was not significantly associated with pulmonary lesions (prevalence ratio [PR] 2.14; 95% CI 0.55-8.38; p = 0.275). Sensitivity analyses using 1:1 matching with cluster-robust variance (PR 3.24; 95% CI 0.65-16.1; p = 0.152) and overlap-weighted doubly robust estimation (PR 2.08; 95% CI 0.42-10.4; p = 0.371) yielded directionally positive but imprecise results. Conclusions: Computed tomography proved valuable for the evaluation of thoracic comorbidities in rabbits with dental disease. Although dental and pulmonary abnormalities were often observed together, a statistically significant association was not established. Larger, prospective studies are warranted to further clarify this relationship.

The objective of this study was to determine if the use of human chorionic gonadotrophin (hCG) and ketoprofen would affect the pregnancy percentages of dairy cows following timed artificial insemination (TAI). This experiment was conducted on a commercial dairy farm in China involving 799 healthy Holstein cows (2-4 parities) between October and December 2024. Cows were randomly assigned to three groups: hCG-3 = treated with (0 d: GnRH, 100 μg; 7-8 d: PGF_2α, 0.4 mg; 52 h: GnRH, 100 μg; 14-16 h: TAI) + hCG 3 vials (300 µg)/cow on day 7 of TAI; hCG-2 group = TAI + 2 vials (200 µg) hCG/cow on day 7; and hCG+ketoprofen = TAI + 300 µg hCG/cow + ketoprofen 10 mL/cow on days 15 and 16 of TAI. Metabolomic profiling (untargeted and targeted) of 22 pregnant cows was conducted on serum collected on days 17 and 21 post-TAI. Results indicated greater pregnancy percentages in the cows of the hCG+ketoprofen-treated group compared to those in the other two groups (60.1% compared with 49.6% and 41.9%). The cows treated with hCG+ketoprofen had less oxidative stress markers, downregulation of arachidonic acid metabolism and upregulation of glycerophospholipid metabolism on day 17 after TAI, indicating that there was upregulation of tryptophan and serotonergic pathways, increased amino acid metabolism and continued anti-inflammatory effects on day 21 after TAI. These findings were confirmed by evaluation data collected by conducting the targeted metabolomic procedures, as indicated by the greater progesterone and melatonin and lesser 17-estradiol and 21-deoxycortisol concentrations. These findings indicate that combined hCG+ketoprofen administrations following the TAI treatment regimen improve pregnancy percentages in dairy cattle as a result of metabolic and endocrine milieu modulations.

Gastrointestinal (GI) parasitic infections pose a formidable global challenge to livestock production and continue to affect livestock health and productivity, particularly in tropical and subtropical regions. This study investigated the prevalence, diversity, and epidemiological determinants of GI parasites in 1406 cattle, goats, and sheep from three districts of North Bengal, India (Cooch Behar, Alipurduar, and Jalpaiguri). Parasitological data were analysed using descriptive statistics and inferential methods. Overall prevalence was 69.4%, with cattle showing the highest infection rate (71.62%), followed by sheep (69.30%) and goats (67.19%). Spatial variation was evident among districts, with Cooch Behar recording the highest prevalence (71.20%). Seasonal effects were assessed using Generalized Linear Mixed Models (GLMs), which indicated significantly higher infection probabilities during the monsoon (75.70%) and summer (72.95%) compared with winter (57.78%). The predominant parasite genera identified were Eimeria spp., Strongyloides spp., and Fasciola spp. Host-parasite associations were further explored using Multiple Correspondence Analysis (MCA), revealing distinct clustering patterns, with cattle associated mainly with Eimeria spp. and Strongyloides spp., goats with Trichuris spp. and Nematodirus spp., and sheep with Fasciola spp. and Paramphistomum spp. A species-specific heatmap was used to visualize parasite distribution across host species and seasons, highlighting higher infection intensities during the summer and monsoon periods. Overall, the results demonstrate that GI parasitic infections in North Bengal are influenced by host species and seasonal climatic factors, supporting the implementation of targeted, species- and season-adapted parasite management strategies.

Infectious Bronchitis Virus is one of several major viral infections in poultry, affecting the respiratory, reproductive, and renal systems and causing significant economic losses worldwide. Current vaccines, including the H120 strain, provide limited cross-protection against emerging variants, underscoring the need for improved vaccine strategies. In this study, the complete genome of IBV H120 was divided into 12 fragments, synthesized, and assembled using the Golden Gate Assembly (GGA) method. The recombinant virus (rH120) was successfully rescued in chicken fibroblast cells and propagated in embryonated specific-pathogen-free (SPF) chicken eggs. Growth kinetics in embryonated SPF chicken eggs revealed similar replication patterns between rH120 and the original H120 strain. In broiler chickens, rH120 replicated efficiently, as confirmed by viral RNA detection in throat and cloacal swabs, and induced a stronger antibody response by 14 days post-infection. The rH120 virus proved to be genetically stable, infectious, and immunogenic, indicating that GGA-based reverse genetics is an effective system for IBV vaccine development.

In livestock farming, the reproductive function and breeding performance of Tan sheep are crucial for enhancing farming efficiency. Despite advances in research on sheep germ cells, studies on the identification of markers for spermatogonia, spermatocytes, and spermatozoa in Tan sheep remain limited and inadequate. In this study, Tan sheep were used as research subjects to investigate the morphological characteristics of testicular tissues, the developmental status of germ cells, and potential novel markers for spermatogonia, spermatocytes, and spermatozoa across different ages (0 days, 60 days, 180 days, and 365 days). Homology of the SMC3, G3BP1, and AKAP4 genes was analyzed via NCBI alignment. The localization and expression characteristics of these genes in the testis tissues of Tan sheep were investigated using HE staining, qPCR, and immunofluorescence double staining. The results showed that from 0 to 365 days of age, with increasing age, spermatogonia, spermatocytes, and spermatids exhibited an orderly distribution, and mature spermatozoa appeared in the tubular lumen, marking the initial establishment of the spermatogenic process. The homology of SMC3, G3BP1, and AKAP4 was 90%, 85%, and 81%. The mRNA levels of SMC3 and G3BP1 in the testes of 60-day-old Tan sheep were significantly increased, while AKAP4 expression showed a gradual increase with advancing age. SMC3 was co-localized with PLZF in undifferentiated spermatogonia, G3BP1 was co-expressed with SYCP2 in spermatocytes, and AKAP4 was co-expressed with PNA in spermatozoa. The findings of this study provide further supportive evidence for novel markers of spermatogonia, spermatocytes, and spermatozoa in Tan sheep.

The metastrongyloids Aelurostrongylus abstrusus and Troglostrongylus brevior, and the capillariid Capillaria aerophila, are major respiratory nematodes infecting cats. In recent decades these nematodes have been recorded in several areas of the world and have called for epizootiological studies to update knowledge on their distribution. While A. abstrusus is known to occur in some areas of Brazil, the presence of T. brevior remains uncertain, and feline infections with C. aerophila have never been reported. This study has investigated the occurrence of lungworms with or without other endoparasitoses in domestic cat populations of selected areas of Brazil. Individual faecal samples were collected from 537 cats, i.e., 521 in metropolitan area of Rio de Janeiro and 16 in Porto Alegre (Rio Grande do Sul). All samples were examined with Sheather's flotation, and 521 of them also with the Baermann test. DNA was extracted from 425 Baermann sediments and molecularly examined for A. abstrusus and T. brevior. The most frequent parasites found at the copromicroscopy were Ancylostomatidae (n. 30, 5.8%), Toxocara cati (14, 2.7%), and Cystoisospora felis (n. 8; 1.5%). Larvae of A. abstrusus were identified by Baermann in 3 samples from Rio de Janeiro, while at PCRs, A. abstrusus DNA was amplified in 10 samples from Rio de Janeiro (n. 3), Seropédica (n. 3), Niterói (n. 3), and Porto Alegre (n. 1). The results indicate that A. abstrusus is enzootic in the investigated areas of Brazil and underline the usefulness of PCR when larvae are not detected by copromicroscopy or when only a single stool sample is analysed. Epizootiological implications, along with the absence of T. brevior in the examined cats and the presence of zoonotic endoparasites, are discussed.

The valorization of cull sheep and the incorporation of agro-industrial by-products into animal feeding represent effective approaches to enhancing the sustainability of small ruminant production systems. This study investigated the effects of dietary inclusion of 17% partially destoned olive cake (OC) in the concentrate fed to Valle del Belice ewes on carcass characteristics, as well as on meat and salami quality. A 14-week feeding trial was conducted on 124 animals allocated to a control (CTR) and an experimental (EXP) group, balanced for parity, days in milk, and daily milk yield. At the end of the trial, five animals per group were slaughtered and their meat was processed into three types of salami: 100% sheep meat (SM), 90% sheep meat with 10% beef heifer brisket (HB), and 90% sheep meat with 10% pork backfat (PB). Meat and salami were evaluated for chemical composition, fatty acid profile, polyphenol content, antioxidant capacity, lipid oxidation, microbiological status, textural properties, and sensory characteristics. Dietary OC supplementation resulted in increased carcass weight, separable fat, intramuscular fat content, and monounsaturated fatty acids-particularly oleic acid-along with higher polyphenol levels and antioxidant activity. Salami produced from OC-fed ewes exhibited reduced weight loss during ripening, lower lipid oxidation, an improved MUFA/SFA ratio, and satisfactory sensory attributes. Microbiological analyses indicated a dominance of lactic acid bacteria and coagulase-negative staphylococci, with no pathogenic microorganisms detected. Overall, the inclusion of olive cake in the diet enhanced meat and processed product quality, supporting the valorization of olive oil by-products within circular economy frameworks.

The slaughter of pregnant sows remains a relevant concern in modern swine production, with prevalence rates reported from 1.5-13% in Europe. Considering fetal sensitivity during late gestation and legal restrictions on transport and slaughter, reliable assessment of fetal age is of considerable practical, ethical, and legal relevance. In this study, 70 pregnancies from low-prolificacy (purebred German Saddleback) and medium-to-high prolificacy genotypes (purebred German Landrace and Duroc × German Landrace hybrids) were repeatedly examined using transabdominal ultrasonography, with a total of 15 examinations per pregnancy. Seven fetometric parameters-rosto-occipital distance, bi-parietal distance, orbital distance, sternum length, thorax diameter, body diameter, and crown-rump length-were measured in vivo, assessing two fetuses per pregnancy and calculating mean values to account for intra-individual variation. Parameter feasibility varied across gestation: during early gestation (gestation days 38 and 40), orbital distance, sternum length, and crown-rump length could be reliably measured; in mid-gestation, all seven parameters were measurable, whereas in late gestation (from gestational day 87 onward), crown-rump length was no longer measurable, and the remaining six parameters remained assessable for gestational age estimation. Crown-rump length (CRL) increased from a median of 3.2 cm (range 1.9-4.2 cm) at day 38 to 16.3 cm (range 14.0-18.2 cm) at day 77, representing the most practical parameter for determining the stage of gestation. Litter size had no significant effect on fetometric growth, except for a weak correlation with thorax diameter at day 77, and parity showed no measurable influence on any parameter. The results show that fetometric values in modern sow genotypes are smaller than those reported in earlier literature, highlighting the need for updated gestational age assessment. These findings provide practical guidance for gestational age estimation, supporting the enforcement of animal welfare legislation and potentially contributing to a reduction in the slaughter of highly pregnant sows.

Members of the microsporidial genus Encephalitozoon have the capacity to infect both mammals and birds, and E. cuniculi is most commonly found in rabbits. With a seroprevalence ranging up to 85%, E. cuniculi can be a problem in pet rabbits as well as in food production and laboratory animal science. While most infections are likely subclinical, there are three main clinical presentations: neurological, renal, and ocular. Typical clinical signs including vestibular disease and phacoclastic uveitis may develop with initial or relapsing infection, while renal infection is usually progressive and associated with non-specific clinical signs. High-sensitivity/specificity ante mortem diagnostic options are lacking, and serological testing most often provides adjunct rather than definitive information such that physical examination and other diagnostics are used more so for ruling out other differentials and comorbidities, rather than confirming infection. In the veterinary community, treatment regimens are variable given the lack of thorough studies and a consensus. The aim of this document is to present the available literature to give a concise review of this organism and its infection of rabbits as well as to propose guidelines and protocols for diagnostics and treatment regimens. In addition, the current challenges and recommendations for further studies are discussed.