The Ability of the Indigenous Bacteria Chromobacterium haemolyticum strain W15 to Degrade Chromium (Cr) and Lead (Pb) in Liquid Coal Waste

Abstract

The use of coal as a raw material for power plants has a good economic impact, but it also has a detrimental environmental impact, particularly due to the presence of Cr and Pb, heavy metals with bioaccumulation and biomagnification qualities. Efforts to control Pb and Cr in liquid coal waste can be achieved by bioremediation. The goal of this study is to screen indigenous bacteria, identify, and test biodegradation on the best bacteria capable of degrading Cr and Pb. Bacterial screening is done experimentally in the lab. Bacterial identification is done using morphological, biochemical, and molecular genetic methods. Using atomic absorption spectroscopy to validate Cr and Pb biodegradation research. Biodegradation experiments revealed that the efficacy of indigenous bacteria reduced Pb by 216% (0.238 ppm to 0.11 ppm) and Cr by 195% (0.34 ppm to 0.174 ppm). The findings of biochemical, morphological, and molecular genetic studies revealed that the top bacterial strains were up to 96% related. using Chromobacterium haemolyticum strain W15. Chromobacterium haemolyticum strain X, an indigenous bacteria capable of degrading Cr and Pb, was successfully isolated from liquid waste.