Reference Gene Selection and Gene Expression Analysis during Gall Development of Zizania latifolia

IF 4.7 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC ACS Applied Electronic Materials Pub Date : 2024-07-18 DOI:10.3390/horticulturae10070759

Yipeng Li, Huan Yi, Qing Gu, Zhaisheng Zheng, Mingxing Zhu, Xiaojun Zha, Shangfa Zhang, Mengfei Yang

引用次数: 0

Abstract

The stem tips of Zizania latifolia at different development stages were used as research materials. The expression stability of nine candidate reference genes (ACT1, H2B, UBI, EF-1α, GAPDH, β-actin, 60S, SKIP and AQP) were evaluated using qRT-PCR. The data were analyzed with GeNorm and NormFinder software. Present results indicated that the expression of ACT1 was stable and that it could be used as the optimal reference gene for studying the development stage of gall formation. ACT1 was selected as the reference gene to verify the expression level of the correlative genes in the gall formation stage of Z. latifolia. Our results were consistent with the previous transcriptome sequencing results. This study revealed that ACT1 was the classic reference gene for the analysis of correlative genes in all of the gall development stages of Z. latifolia.

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瘿发育过程中的参考基因选择和基因表达分析

以不同发育阶段的茎尖为研究材料。采用 qRT-PCR 方法评估了九个候选参考基因（ACT1、H2B、UBI、EF-1α、GAPDH、β-actin、60S、SKIP 和 AQP）的表达稳定性。数据使用 GeNorm 和 NormFinder 软件进行分析。结果表明，ACT1 的表达稳定，可作为研究虫瘿形成发育阶段的最佳参考基因。我们选择 ACT1 作为参考基因，以验证相关基因在花叶浙贝母虫瘿形成期的表达水平。我们的结果与之前的转录组测序结果一致。这项研究表明，ACT1 是分析花叶泽兰所有虫瘿发育阶段相关基因的经典参考基因。

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来源期刊

ACS Applied Electronic Materials Multiple-

CiteScore

7.20

自引率

4.30%

发文量

567

期刊介绍： ACS Applied Electronic Materials is an interdisciplinary journal publishing original research covering all aspects of electronic materials. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials science, engineering, optics, physics, and chemistry into important applications of electronic materials. Sample research topics that span the journal's scope are inorganic, organic, ionic and polymeric materials with properties that include conducting, semiconducting, superconducting, insulating, dielectric, magnetic, optoelectronic, piezoelectric, ferroelectric and thermoelectric. Indexed/Abstracted： Web of Science SCIE Scopus CAS INSPEC Portico

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