Huong Nguyen Thi, Thuy Do Ngoc, Van Trung Phung, Hung Le Ngoc, van Nam Mai
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引用次数: 0
Abstract
The medicinal herb Asparagus racemosus has been used in traditional medicine to treat various diseases such as cough, diarrhoea, diabetes, gastric issues, gonorrhoea, headaches, piles, rheumatism, and even lactation enhancement. This study explores the genetic information and phytochemicals of the species native to Gia Lai province, Vietnam before its conservation and cultivation. Five species of A. racemosus were analyzed using the trnL-e/trnL-f regions sequence. A. racemosus roots were extracted by water then Ultra-High-Performance Liquid Chromatography coupled with Quadrupole/Time-Of-Flight Mass Spectrometry (UHPLC-Q-TOF-MS) spectroscopy was used to screen for their phytochemicals. We have confirmed the DNA genetics of A. racemosus species collected in Gia Lai, Vietnam. In water extract of A. racemosus roots, UHPLC-Q-TOF-MS tentatively identified two flavonoids (Quercetin-3-glucuronide, Rutin), five steroidal saponins (Shatavarin I, Shatavarin IV, Shatavarin IX, Asparacoside, Asparanin A), and two steroids (β-sitosterol, Daucosterol). The experimental findings confirm the A. racemosus species for conservation and cultivation in Vietnam and contribute the benefits to the chemical literature of Vietnamese natural flora. A. racemosus should be further studied for pharmaceutical activities.
在传统医学中,药用植物天门冬被用来治疗各种疾病,如咳嗽、腹泻、糖尿病、胃病、淋病、头痛、痔疮、风湿病,甚至还能催乳。本研究探讨了原产于越南嘉莱省的该物种在保护和种植前的遗传信息和植物化学成分。研究利用 trnL-e/trnL-f 区域序列分析了五种 A. racemosus。通过水提取外消旋草根,然后使用超高效液相色谱-四极杆/飞行时间质谱(UHPLC-Q-TOF-MS)光谱法筛选其植物化学物质。我们确认了在越南嘉莱采集的 A. racemosus 的 DNA 遗传学特征。在A. racemosus根的水提取物中,超高效液相色谱-Q-TOF-MS初步鉴定出两种黄酮类化合物(槲皮素-3-葡萄糖醛酸苷、芦丁)、五种甾体皂甙(沙他苷 I、沙他苷 IV、沙他苷 IX、天冬苷、天冬皂苷 A)和两种甾体化合物(β-谷甾醇、Daucosterol)。实验结果证实了越南可以保护和栽培A. racemosus物种,并为越南天然植物区系的化学文献做出了贡献。应进一步研究 A. racemosus 的制药活性。