Development of a sensitive ds-DNA/Au NPs/ PGE biosensor for determination of Buprenorphine using electrochemical and molecular dynamic simulation investigation

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioelectrochemistry Pub Date : 2024-07-26 DOI:10.1016/j.bioelechem.2024.108783

Fereshteh Golestanifar , Zahra Garkani-Nejad , Shahab Maghsoudi

{"title":"Development of a sensitive ds-DNA/Au NPs/ PGE biosensor for determination of Buprenorphine using electrochemical and molecular dynamic simulation investigation","authors":"Fereshteh Golestanifar , Zahra Garkani-Nejad , Shahab Maghsoudi","doi":"10.1016/j.bioelechem.2024.108783","DOIUrl":null,"url":null,"abstract":"<div><p>A sensitive electrochemical DNA biosensor has been developed for the detection of Buprenorphine (Bu), a narcotic pain reliever. To achieve this, double-stranded DNA (ds-DNA) was immobilized on a pencil graphite electrode that was modified with gold nanoparticles (Au NPs/PGE). The gold nanoparticles enhanced the performance of the DNA biosensor. The constructed ds-DNA/Au NPs/ PGE exhibited a linear detection range spanning from 0.05 to 100 μM with an impressive detection limit of 20 nM for Bu detection. Additionally, the DNA biosensor demonstrated good response in real samples evaluations. Finally, the interaction between carbon and gold atoms with DNA was confirmed through molecular dynamics simulation, while the interaction between DNA and the Bu drug was confirmed through molecular docking method. In conclusion, the electrochemical DNA biosensor presented in this study demonstrates exceptional sensitivity and reliability in the detection of buprenorphine. The incorporation of gold nanoparticles, as well as the use of molecular dynamics simulations and docking methods, contributes to a comprehensive understanding of the interactions involved in this detection process.</p></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"160 ","pages":"Article 108783"},"PeriodicalIF":4.8000,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioelectrochemistry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1567539424001452","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

A sensitive electrochemical DNA biosensor has been developed for the detection of Buprenorphine (Bu), a narcotic pain reliever. To achieve this, double-stranded DNA (ds-DNA) was immobilized on a pencil graphite electrode that was modified with gold nanoparticles (Au NPs/PGE). The gold nanoparticles enhanced the performance of the DNA biosensor. The constructed ds-DNA/Au NPs/ PGE exhibited a linear detection range spanning from 0.05 to 100 μM with an impressive detection limit of 20 nM for Bu detection. Additionally, the DNA biosensor demonstrated good response in real samples evaluations. Finally, the interaction between carbon and gold atoms with DNA was confirmed through molecular dynamics simulation, while the interaction between DNA and the Bu drug was confirmed through molecular docking method. In conclusion, the electrochemical DNA biosensor presented in this study demonstrates exceptional sensitivity and reliability in the detection of buprenorphine. The incorporation of gold nanoparticles, as well as the use of molecular dynamics simulations and docking methods, contributes to a comprehensive understanding of the interactions involved in this detection process.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

利用电化学和动态模拟研究开发用于测定丁丙诺啡的灵敏ds-DNA/Au NPs/ PGE 生物传感器

我们开发了一种灵敏的电化学 DNA 生物传感器，用于检测丁丙诺啡 (Bu)（一种麻醉性镇痛药）。为此，双链 DNA（ds-DNA）被固定在用金纳米粒子（Au NPs/PGE）修饰过的铅笔状石墨电极上。金纳米粒子增强了 DNA 生物传感器的性能。所构建的 ds-DNA/Au NPs/PGE 的线性检测范围为 0.05 至 100 μM，对 Bu 的检测限为 20 nM，令人印象深刻。此外，DNA 生物传感器在实际样品评估中表现出良好的响应。最后，通过分子动力学模拟证实了碳原子和金原子与 DNA 之间的相互作用，同时通过分子对接方法证实了 DNA 与 Bu 药物之间的相互作用。总之，本研究提出的电化学 DNA 生物传感器在检测丁丙诺啡方面表现出了极高的灵敏度和可靠性。金纳米粒子的加入以及分子动力学模拟和对接方法的使用，有助于全面了解这一检测过程中的相互作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.