Biocontrol potential of Bacillus velezensis HG-8-2 against postharvest anthracnose on chili pepper caused by Colletotrichum scovillei

IF 4.5 1区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Food microbiology Pub Date : 2024-07-26 DOI:10.1016/j.fm.2024.104613
Jie Zhong, Xiao Wu, Rui Guo, Jiaxin Li, Xiaogang Li, Junzi Zhu
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Abstract

Anthracnose caused by Colletotrichum scovillei is a significant disease of pepper, including in postharvest stage. Bacillus species represent a potential microbial resource for controlling postharvest plant diseases. Here, a strain HG-8-2 was obtained and identified as Bacillus velezensis through morphological, biochemical, physiological, and molecular analyses. The culture filtrate showed highly antifungal activity against C. scovillei both in vitro and on pepper fruit. Crude lipopeptide extracts, which had excellent stability, could effectively inhibit mycelial growth of C. scovillei with an EC50 value of 28.48 ± 1.45 μg mL−1 and inhibited conidial germination. Pretreatment with the extracts reduced the incidence and lesion size of postharvest anthracnose on pepper fruit. Analysis using propidium iodide staining, malondialdehyde content detection and scanning electron microscope observation suggested that the crude lipopeptide extracts harbored antifungal activity by damaging cell membranes and mycelial structures. The RNA-seq analysis conducted on C. scovillei samples treated with the extracts, as compared to untreated samples, revealed significant alterations in the expression of multiple genes involved in protein biosynthesis. Overall, these results demonstrated that B. velezensis HG-8-2 and its crude lipopeptide extracts exhibit highly antagonistic ability against C. scovillei, thereby offering an effective biological agent for the control of anthracnose in pepper fruit.

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枯草芽孢杆菌 HG-8-2 对由 Colletotrichum scovillei 引起的辣椒收获后炭疽病的生物防治潜力
由 Colletotrichum scovillei 引起的炭疽病是辣椒的一种重要病害,包括在收获后阶段。芽孢杆菌是控制收获后植物病害的潜在微生物资源。本文获得了一株 HG-8-2 菌株,并通过形态学、生物化学、生理学和分子分析确定其为 Velezensis 杆菌。其培养滤液在体外和辣椒果实上对 Scovillei 真菌都表现出很强的抗真菌活性。具有良好稳定性的粗脂肽提取物能有效抑制 C. scovillei 的菌丝生长,EC50 值为 28.48 ± 1.45 μg mL-1,并能抑制分生孢子的萌发。用提取物进行预处理可减少辣椒果实采后炭疽病的发病率和病变面积。利用碘化丙啶染色、丙二醛含量检测和扫描电子显微镜观察进行的分析表明,粗脂肽提取物具有破坏细胞膜和菌丝结构的抗真菌活性。与未处理的样本相比,对使用提取物处理的 C. scovillei 样本进行的 RNA-seq 分析表明,参与蛋白质生物合成的多个基因的表达发生了显著变化。总之,这些结果表明 B. velezensis HG-8-2 及其粗脂肽提取物对 C. scovillei 具有很强的拮抗能力,从而为控制辣椒果实炭疽病提供了一种有效的生物制剂。
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来源期刊
Food microbiology
Food microbiology 工程技术-生物工程与应用微生物
CiteScore
11.30
自引率
3.80%
发文量
179
审稿时长
44 days
期刊介绍: Food Microbiology publishes original research articles, short communications, review papers, letters, news items and book reviews dealing with all aspects of the microbiology of foods. The editors aim to publish manuscripts of the highest quality which are both relevant and applicable to the broad field covered by the journal. Studies must be novel, have a clear connection to food microbiology, and be of general interest to the international community of food microbiologists. The editors make every effort to ensure rapid and fair reviews, resulting in timely publication of accepted manuscripts.
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