Luca Farinelli , Francesco D'Angelo , Carlo Ciccullo , Sandra Manzotti , Antonio Gigante
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引用次数: 0
Abstract
Objective
Aim of the present study was to compare the presence of Mast Cells (MCs) in synovial samples from gleno-humeral osteoarthritis (OA) and from control group.
Methods
Synovial tissue samples were obtained during arthroplasty from 23 patients with gleno-humeral OA due to rotator cuff arthropathy (RCA) and from 20 patients without OA, constituting OA group and control group respectively. Before surgery self-reported pain was assessed using VAS score and OSS was used to value functional ability. Shoulder radiograph (Antero-posterior, Y-view and Grashey views) was evaluated by musculoskeletal radiologist and graded according to modified Samilson-Prieto classification.
Synovial tissue, obtained during arthroplasty and arthroscopic procedure, was prepared to immunohistochemical analysis with anti-CD31 and anti-CD117 antibodies, to detect respectively endothelial cells and MCs at 40x magnification. Synovitis scores have been assessed. Under the control of the image processing system the distribution and the total number of vessels and MCs were determined.
Results
The numbers of MCs and the area fraction (20x magnification) occupied by them were significantly higher in OA samples than in control tissue. The synovitis score was higher in OA patients with a positive correlation. Vessels number and area fraction were higher in OA patients than in controls. Analysis of MC number in relation to clinical data indicated positive correlation with the VAS score.
Conclusions
The distribution of MCs on synovium significantly differ between OA and control groups. Despite the design of the study could not conclude the cause-effect relationship, the presence of MCs might have role in OA pathogenesis.
本研究旨在比较盂肱骨关节炎（OA）滑膜样本和对照组滑膜样本中肥大细胞（MC）的存在情况。方法分别从23例因肩袖关节病（RCA）引起的盂肱骨关节炎患者和20例无OA的患者（OA组和对照组）的关节成形术中获取滑膜组织样本。手术前使用 VAS 评分评估患者的自我疼痛报告，并使用 OSS 评估患者的功能能力。在关节置换术和关节镜手术中获得的滑膜组织，用抗CD31和抗CD117抗体进行免疫组化分析，以40倍放大率分别检测内皮细胞和MCs。滑膜炎评分已被评估。在图像处理系统的控制下，确定了血管和 MC 的分布和总数。OA 患者的滑膜炎评分较高，且呈正相关。OA患者的血管数量和面积分数均高于对照组。与临床数据相关的 MC 数量分析表明与 VAS 评分呈正相关。尽管该研究的设计不能得出因果关系的结论，但MCs的存在可能与OA的发病机制有关。
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