Review of embedding and non-embedding techniques for quantitative wood anatomy

IF 2.7 3区 农林科学 Q1 FORESTRY Dendrochronologia Pub Date : 2024-07-18 DOI:10.1016/j.dendro.2024.126241
Davide Frigo , Philipp Römer , Lucrezia Unterholzner , Heike Zimmer-Zachmann , Jan Esper , Marco Carrer , Emanuele Ziaco
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Abstract

In recent decades, xylem anatomical traits have become increasingly important in dendrochronological research, as they offer the unique opportunity to assess eco-physiological drivers of tree growth at intra-annual resolution. However, standard protocols for generating such data are still missing, leading to methodological uncertainty, and complicating data exchange among laboratories. Here, we compare protocols for high-quality permanent slide preparation in dendroanatomy and address the effects of paraffin embedding vs. non-embedding approaches. Tests are conducted on both gymnosperm and angiosperm wood types of widely distributed European tree species, considering cell wall thickness (CWT), mean lumen area (MLA), and hydraulic diameter (Dh). Results indicate that non-embedding does not significantly alter the qualitative and quantitative characteristics of permanent slides compared to embedded samples. Whereas the mean chronologies of MLA and Dh and their non-embedded counterparts share substantial high-frequency variance, the CWT chronologies reveal slightly larger discrepancies at inter-annual scale. However, methodological differences do not exceed 11.1 % for any parameter. While these results show high similarity between the two approaches, we recommend adopting the non-embedding procedure, since it saves resources and therefore allows to produce larger datasets. Regardless of the protocol used to build wood anatomical datasets, assembling large-scale networks of wood anatomical data could transform our understanding of forest responses to global changes.

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木材定量解剖包埋和非包埋技术综述
近几十年来,木质部解剖特征在树木年代学研究中变得越来越重要,因为它们提供了以年内分辨率评估树木生长的生态生理驱动因素的独特机会。然而,生成此类数据的标准协议仍然缺失,导致了方法上的不确定性,并使实验室之间的数据交换变得更加复杂。在此,我们比较了树皮解剖学中高质量永久切片制备的规程,并探讨了石蜡包埋与非包埋方法的影响。我们对广泛分布的欧洲树种的裸子植物和被子植物木材类型进行了测试,并考虑了细胞壁厚度(CWT)、平均管腔面积(MLA)和水力直径(Dh)。结果表明,与嵌入样本相比,非嵌入不会明显改变永久切片的定性和定量特征。虽然 MLA 和 Dh 的平均年表与未嵌入样本的平均年表在高频率上有很大差异,但 CWT 年表在年际尺度上的差异略大。不过,任何参数的方法差异都不超过 11.1%。虽然这些结果表明这两种方法具有很高的相似性,但我们建议采用非嵌入式程序,因为它可以节省资源,从而生成更大的数据集。无论采用哪种方法建立木材解剖数据集,组建大规模的木材解剖数据网络都能改变我们对森林对全球变化的反应的认识。
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来源期刊
Dendrochronologia
Dendrochronologia FORESTRY-GEOGRAPHY, PHYSICAL
CiteScore
5.50
自引率
13.30%
发文量
82
审稿时长
22.8 weeks
期刊介绍: Dendrochronologia is a peer-reviewed international scholarly journal that presents high-quality research related to growth rings of woody plants, i.e., trees and shrubs, and the application of tree-ring studies. The areas covered by the journal include, but are not limited to: Archaeology Botany Climatology Ecology Forestry Geology Hydrology Original research articles, reviews, communications, technical notes and personal notes are considered for publication.
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