Diverse mechanisms confer bensulfuron-methyl resistance in Schoenoplectiella juncoides (Roxb.) lye

IF 4.2 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pesticide Biochemistry and Physiology Pub Date : 2024-07-20 DOI:10.1016/j.pestbp.2024.106034

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Abstract

The effectiveness of bensulfuron-methyl in controlling Schoenoplectiella juncoides (Roxb.) Lye has significantly decreased in rice fields in China. Hence, a bensulfuron-methyl-resistant S. juncoides population (W15) was collected from Dandong City, Liaoning Province, China, to investigate the underlying resistance mechanisms. Whole-plant dose-response experiments and ALS activity assay confirmed that W15 has evolved high-level resistance to bensulfuron-methyl compared with the susceptible S. juncoides population (W4). Molecular analysis revealed a Pro-197-Ser mutation in ALS1, while there was no significant difference in the relative ALS gene expression between W15 and W4. LC-MS/MS analysis showed W15 metabolized bensulfuron-methyl more rapidly than W4. Furthermore, bensulfuron-methyl resistance in W15 was significantly alleviated by malathion and 4-chloro-7-nitrobenzoxadiazole (NBD-Cl). Glutathione S-transferase activity was higher in W15 than in W4. Meanwhile, W15 displayed cross-resistance to halosulfuron-methyl and multi-resistance to MCPA-Na. In summary, these findings demonstrated for the first time that both target- and non-target-site resistance are relevant in the resistance of S. juncoides to bensulfuron-methyl.

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多种机制赋予苄嘧磺隆（Schoenoplectiella juncoides (Roxb.) lye）抗性

在中国的稻田中，苄嘧磺隆防治褐飞虱的效果显著下降。因此，研究人员从中国辽宁省丹东市采集了一个苄嘧磺隆抗性禾本科禾本科白蛾（S. juncoides）种群（W15），以研究其潜在的抗性机制。全株剂量反应实验和 ALS 活性测定证实，与易感苄嘧磺隆的桧柏种群（W4）相比，W15 对苄嘧磺隆具有高水平的抗性。分子分析表明，W15 和 W4 的 ALS 基因表达没有显著差异。LC-MS/MS 分析表明，W15 比 W4 对苄嘧磺隆的代谢速度更快。此外，马拉硫磷和 4-氯-7-硝基苯并噁二唑（NBD-Cl）能显著缓解 W15 对苄嘧磺隆的抗性。W15 的谷胱甘肽 S 转移酶活性高于 W4。同时，W15 显示出对甲基吡嘧磺隆的交叉抗性和对 MCPA-Na 的多重抗性。总之，这些研究结果首次证明了靶标抗性和非靶标抗性都与俊草对苄嘧磺隆的抗性有关。

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来源期刊

Pesticide Biochemistry and Physiology 生物-昆虫学

CiteScore

7.00

自引率

8.50%

发文量

238

审稿时长

4.2 months

期刊介绍： Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance. Research Areas Emphasized Include the Biochemistry and Physiology of: • Comparative toxicity • Mode of action • Pathophysiology • Plant growth regulators • Resistance • Other effects of pesticides on both parasites and hosts.