Functional Identification and Genetic Analysis of O-Antigen Gene Clusters of Food-Borne Pathogen Yersinia enterocolitica O:10 and Other Uncommon Serotypes, Further Revealing Their Virulence Profiles.

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of microbiology and biotechnology Pub Date : 2024-08-28 Epub Date: 2024-07-15 DOI:10.4014/jmb.2402.02044
Bin Hu, Jing Wang, Linxing Li, Qin Wang, Jingliang Qin, Yingxin Chi, Junxiang Yan, Wenkui Sun, Boyang Cao, Xi Guo
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Abstract

Yersinia enterocolitica is a globally distributed food-borne gastrointestinal pathogen. The O-antigen variation-determined serotype is an important characteristic of Y. enterocolitica, allowing intraspecies classification for diagnosis and epidemiology purposes. Among the 11 serotypes associated with human yersiniosis, O:3, O:5,27, O:8, and O:9 are the most prevalent, and their O-antigen gene clusters have been well defined. In addition to the O-antigen, several virulence factors are involved in infection and pathogenesis of Y. enterocolitica strains, and these are closely related to their biotypes, reflecting pathogenic properties. In this study, we identified the O-AGC of a Y. enterocolitica strain WL-21 of serotype O:10, and confirmed its functionality in O-antigen synthesis. Furthermore, we analyzed in silico the putative O-AGCs of uncommon serotypes, and found that the O-AGCs of Y. enterocolitica were divided into two genetic patterns: (1) O-AGC within the hemH-gsk locus, possibly synthesizing the O-antigen via the Wzx/Wzy dependent pathway, and (2) O-AGC within the dcuC-galU-galF locus, very likely assembling the O-antigen via the ABC transporter dependent pathway. By screening the virulence genes against genomes from GenBank, we discovered that strains representing different serotypes were grouped according to different virulence gene profiles, indicating strong links between serotypes and virulence markers and implying an interaction between them and the synergistic effect in pathogenicity. Our study provides a framework for further research on the origin and evolution of O-AGCs from Y. enterocolitica, as well as on differences in virulent mechanisms among distinct serotypes.

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食源性病原体小肠结肠耶尔森菌 O:10 和其他不常见血清型 O 抗原基因簇的功能鉴定和遗传分析,进一步揭示其毒性特征
小肠结肠耶尔森菌是一种全球分布的食源性胃肠道病原体。由 O 抗原变异决定的血清型是小肠结肠炎耶尔森菌的一个重要特征,可用于种内诊断和流行病学分类。在与人类耶尔森氏菌病相关的 11 个血清型中,O:3、O:5,27、O:8 和 O:9 最为流行,它们的 O 抗原基因簇已被明确定义。除 O 抗原外,还有几种毒力因子参与了小肠结肠炎 Y. 菌株的感染和致病过程,这些毒力因子与其生物型密切相关,反映了致病特性。在本研究中,我们鉴定了血清型为 O:10 的小肠结肠炎 Y. 菌株 WL-21 的 O-AGC,并证实了其在 O 抗原合成中的功能。此外,我们还对不常见血清型的假定 O-AGC 进行了硅学分析,发现小肠结肠炎酵母菌的 O-AGC 可分为两种遗传模式:(1)位于 hemH-gsk 基因座内的 O-AGC,可能通过 Wzx/Wzy 依赖途径合成 O 抗原;(2)位于 dcuC-galU-galF 基因座内的 O-AGC,很可能通过 ABC 转运体依赖途径组装 O 抗原。通过对照 GenBank 中的基因组筛选毒力基因,我们发现代表不同血清型的菌株根据不同的毒力基因图谱进行了分组,这表明血清型与毒力标记之间存在密切联系,并意味着它们之间存在相互作用和致病性协同效应。我们的研究为进一步研究小肠结肠炎病毒 O-AGCs 的起源和进化以及不同血清型之间致病机制的差异提供了一个框架。
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来源期刊
Journal of microbiology and biotechnology
Journal of microbiology and biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
5.50
自引率
3.60%
发文量
151
审稿时长
2 months
期刊介绍: The Journal of Microbiology and Biotechnology (JMB) is a monthly international journal devoted to the advancement and dissemination of scientific knowledge pertaining to microbiology, biotechnology, and related academic disciplines. It covers various scientific and technological aspects of Molecular and Cellular Microbiology, Environmental Microbiology and Biotechnology, Food Biotechnology, and Biotechnology and Bioengineering (subcategories are listed below). Launched in March 1991, the JMB is published by the Korean Society for Microbiology and Biotechnology (KMB) and distributed worldwide.
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