Wireless Optogenetic Targeting Nociceptors Helps Host Cells Win the Competitive Colonization in Implant-Associated Infections.

IF 10.7 2区材料科学 Q1 CHEMISTRY, PHYSICAL Small Methods Pub Date : 2024-08-01 DOI:10.1002/smtd.202400216

Xinyu Fang, Haiqi Ding, Yang Chen, Qijin Wang, Xuhui Yuan, Chaofan Zhang, Jiagu Huang, Jiexin Huang, Jianhua Lv, Hongxin Hu, Changyu Huang, Xueni Hu, Yiming Lin, Nanxin Zhang, Wei Zhou, Ying Huang, Wenbo Li, Susheng Niu, Zhaoyang Wu, Jianhua Lin, Bin Yang, Tifei Yuan, Wenming Zhang

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Abstract

The role of nociceptive nerves in modulating immune responses to harmful stimuli via pain or itch induction remains controversial. Compared to conventional surgery, various implant surgeries are more prone to infections even with low bacterial loads. In this study, an optogenetic technique is introduced for selectively activating peripheral nociceptive nerves using a fully implantable, wirelessly rechargeable optogenetic device. By targeting nociceptors in the limbs of awake, freely moving mice, it is found that activation induces anticipatory immunity in the innervated territory and enhances the adhesion of various host cells to the implant surface. This effect mediates acute immune cell-mediated killing of Staphylococcus aureus on implants and enables the host to win "implant surface competition" against Staphylococcus aureus. This finding provides new strategies for preventing and treating implant-associated infections.

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无线光遗传学靶向痛觉感受器帮助宿主细胞在植入物相关感染的竞争性定植中获胜

痛觉神经通过诱导疼痛或瘙痒来调节对有害刺激的免疫反应，这一作用仍存在争议。与传统手术相比，各种植入手术即使细菌量较少，也更容易发生感染。本研究介绍了一种光遗传学技术，利用可无线充电的全植入式光遗传学装置，选择性地激活外周痛觉神经。通过瞄准清醒、自由活动的小鼠四肢的痛觉感受器，研究发现，激活可诱导神经支配区域的预期免疫，并增强各种宿主细胞对植入物表面的粘附。这种效应介导了免疫细胞介导的对植入物上金黄色葡萄球菌的急性杀伤，并使宿主在与金黄色葡萄球菌的 "植入物表面竞争 "中获胜。这一发现为预防和治疗植入物相关感染提供了新策略。

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来源期刊

Small Methods Materials Science-General Materials Science

CiteScore

17.40

自引率

1.60%

发文量

347

期刊介绍： Small Methods is a multidisciplinary journal that publishes groundbreaking research on methods relevant to nano- and microscale research. It welcomes contributions from the fields of materials science, biomedical science, chemistry, and physics, showcasing the latest advancements in experimental techniques. With a notable 2022 Impact Factor of 12.4 (Journal Citation Reports, Clarivate Analytics, 2023), Small Methods is recognized for its significant impact on the scientific community. The online ISSN for Small Methods is 2366-9608.