Evaluation of the enzymatic properties of DNA (cytosine-5)-methyltransferase M.ApeKI from archaea in the presence of metal ions.

IF 1.4 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-09-20 DOI:10.1093/bbb/zbae106
Mao Hayashi, Yoshinari Wada, Akira Yamamura, Hideki Inoue, Naoya Yamashita, Shigetoshi Ichimura, Yasuhiro Iida
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Abstract

We previously identified M.ApeKI from Aeropyum pernix K1 as a highly thermostable DNA (cytosine-5)-methyltransferase. M.ApeKI uses the type II restriction-modification system (R-M system), among the best-studied R-M systems. Although endonucleases generally utilize Mg (II) as a cofactor, several reports have shown that MTases exhibit different reactions in the presence of metal ions. This study aim was to evaluate the enzymatic properties of DNA (cytosine-5)-methyltransferase M.ApeKI from archaea in the presence of metal ions. We evaluated the influence of metal ions on the catalytic activity and DNA binding of M.ApeKI. The catalytic activity was inhibited by Cu (II), Mg (II), Mn (II), and Zn (II), each at 5 m m. DNA binding was more strongly inhibited by 5 m m Cu (II) and 10 m m Zn (II). To our knowledge, this is the first report showing that DNA binding of type II MTase is inhibited by metal ions.

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评估古细菌 DNA(胞嘧啶-5)甲基转移酶 M.ApeKI 在金属离子存在下的酶特性。
此前,我们从Aeropyum pernix K1中发现了M.ApeKI,它是一种高热稳定性DNA(胞嘧啶-5)甲基转移酶。M.ApeKI 使用的是 II 型限制性修饰系统(R-M 系统),是研究得最清楚的 R-M 系统之一。虽然内切酶通常使用镁(II)作为辅助因子,但一些报告显示,MT 酶在金属离子存在的情况下会表现出不同的反应。本研究旨在评估古细菌 DNA(胞嘧啶-5)甲基转移酶 M.ApeKI 在金属离子存在下的酶学特性。我们评估了金属离子对 M.ApeKI 催化活性和 DNA 结合的影响。Cu (II)、Mg (II)、Mn (II)和 Zn (II)(各为 5 mM)抑制了催化活性。5 mM Cu (II) 和 10 mM Zn (II) 对 DNA 结合的抑制作用更强。据我们所知,这是首次报道 II 型 MT 酶的 DNA 结合受到金属离子的抑制。
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来源期刊
Bioscience, Biotechnology, and Biochemistry
Bioscience, Biotechnology, and Biochemistry 生物-生化与分子生物学
CiteScore
3.50
自引率
0.00%
发文量
183
审稿时长
1 months
期刊介绍: Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).
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