Dual role of PpV in Drosophila crystal cell proliferation and survival.

IF 5.3 2区 生物学 Q2 CELL BIOLOGY Journal of Molecular Cell Biology Pub Date : 2024-07-31 DOI:10.1093/jmcb/mjae028
Wang Luo, Fang Zhang, Fangzhen Zhao, Yang Fang, Long Zhao, Ying Su
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Abstract

Drosophila melanogaster crystal cells are a specialized type of blood cells for innate immune process upon injury. Under normal conditions, crystal cells rarely proliferate and constitute a small proportion of fly blood cells. Notch signaling has been known to guide the cell fate determination of crystal cells and maintain their survival. Here, we reported that protein phosphatase V (PpV), the unique catalytic subunit of protein phosphatase 6 in Drosophila, is a novel regulator of crystal cell proliferation and integrity. We found that PpV proteins highly accumulated in crystal cells in the larval hematopoietic organ termed the lymph gland. Silencing PpV using RNA interference led to increased crystal cell proliferation in a Notch-independent manner and induced crystal cell rupture dependent on Notch signaling. Moreover, additive PpV prevented the rupture of crystal cells in lymph glands upon a needle injury, suggesting the involvement of PpV in wound healing. Altogether, our results indicated that PpV plays a dual role in lymph glands, preventing crystal cell proliferation to limit the cell number, as well as inhibiting crystal cell rupture to maintain their survival.

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PpV 在果蝇晶体细胞增殖和存活中的双重作用
黑腹果蝇晶体细胞是一种特化的血细胞,用于受伤后的先天免疫过程。在正常情况下,晶体细胞很少增殖,只占蝇血细胞的一小部分。众所周知,Notch 信号可引导晶体细胞的命运决定并维持其存活。在这里,我们报告了蛋白磷酸酶 V(PpV),果蝇蛋白磷酸酶 6 的独特催化亚基,是晶体细胞增殖和完整性的新型调节因子。我们发现,PpV 蛋白在幼虫造血器官(即淋巴腺)的晶体细胞中高度积累。利用 RNA 干扰沉默 PpV 可导致晶体细胞增殖增加,但不依赖于 Notch 信号,晶体细胞破裂也依赖于 Notch 信号。此外,添加 PpV 可防止针刺伤后淋巴腺中晶体细胞的破裂,这表明 PpV 参与了伤口愈合。总之,我们的研究结果表明,PpV 在淋巴腺中发挥着双重作用,既能阻止晶体细胞增殖以限制细胞数量,又能抑制晶体细胞破裂以维持其存活。
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来源期刊
CiteScore
9.60
自引率
1.80%
发文量
1383
期刊介绍: The Journal of Molecular Cell Biology ( JMCB ) is a full open access, peer-reviewed online journal interested in inter-disciplinary studies at the cross-sections between molecular and cell biology as well as other disciplines of life sciences. The broad scope of JMCB reflects the merging of these life science disciplines such as stem cell research, signaling, genetics, epigenetics, genomics, development, immunology, cancer biology, molecular pathogenesis, neuroscience, and systems biology. The journal will publish primary research papers with findings of unusual significance and broad scientific interest. Review articles, letters and commentary on timely issues are also welcome. JMCB features an outstanding Editorial Board, which will serve as scientific advisors to the journal and provide strategic guidance for the development of the journal. By selecting only the best papers for publication, JMCB will provide a first rate publishing forum for scientists all over the world.
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