The centromere landscapes of four karyotypically diverse Papaver species provide insights into chromosome evolution and speciation.

IF 11.1 Q1 CELL BIOLOGY Cell genomics Pub Date : 2024-08-14 Epub Date: 2024-07-30 DOI:10.1016/j.xgen.2024.100626
Shenghan Gao, Yanyan Jia, Hongtao Guo, Tun Xu, Bo Wang, Stephen J Bush, Shijie Wan, Yimeng Zhang, Xiaofei Yang, Kai Ye
{"title":"The centromere landscapes of four karyotypically diverse Papaver species provide insights into chromosome evolution and speciation.","authors":"Shenghan Gao, Yanyan Jia, Hongtao Guo, Tun Xu, Bo Wang, Stephen J Bush, Shijie Wan, Yimeng Zhang, Xiaofei Yang, Kai Ye","doi":"10.1016/j.xgen.2024.100626","DOIUrl":null,"url":null,"abstract":"<p><p>Understanding the roles played by centromeres in chromosome evolution and speciation is complicated by the fact that centromeres comprise large arrays of tandemly repeated satellite DNA, which hinders high-quality assembly. Here, we used long-read sequencing to generate nearly complete genome assemblies for four karyotypically diverse Papaver species, P. setigerum (2n = 44), P. somniferum (2n = 22), P. rhoeas (2n = 14), and P. bracteatum (2n = 14), collectively representing 45 gapless centromeres. We identified four centromere satellite (cenSat) families and experimentally validated two representatives. For the two allopolyploid genomes (P. somniferum and P. setigerum), we characterized the subgenomic distribution of each satellite and identified a \"homogenizing\" phase of centromere evolution in the aftermath of hybridization. An interspecies comparison of the peri-centromeric regions further revealed extensive centromere-mediated chromosome rearrangements. Taking these results together, we propose a model for studying cenSat competition after hybridization and shed further light on the complex role of the centromere in speciation.</p>","PeriodicalId":72539,"journal":{"name":"Cell genomics","volume":" ","pages":"100626"},"PeriodicalIF":11.1000,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11406182/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell genomics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.xgen.2024.100626","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/7/30 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Understanding the roles played by centromeres in chromosome evolution and speciation is complicated by the fact that centromeres comprise large arrays of tandemly repeated satellite DNA, which hinders high-quality assembly. Here, we used long-read sequencing to generate nearly complete genome assemblies for four karyotypically diverse Papaver species, P. setigerum (2n = 44), P. somniferum (2n = 22), P. rhoeas (2n = 14), and P. bracteatum (2n = 14), collectively representing 45 gapless centromeres. We identified four centromere satellite (cenSat) families and experimentally validated two representatives. For the two allopolyploid genomes (P. somniferum and P. setigerum), we characterized the subgenomic distribution of each satellite and identified a "homogenizing" phase of centromere evolution in the aftermath of hybridization. An interspecies comparison of the peri-centromeric regions further revealed extensive centromere-mediated chromosome rearrangements. Taking these results together, we propose a model for studying cenSat competition after hybridization and shed further light on the complex role of the centromere in speciation.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
四个核型不同的 Papaver 物种的中心粒景观为染色体进化和物种分化提供了启示。
由于中心粒由大量串联重复的卫星 DNA 阵列组成,阻碍了高质量的组装,因此了解中心粒在染色体进化和物种分化中的作用变得更加复杂。在这里,我们利用长线程测序技术为四个核型不同的巴布亚草物种(P. setigerum (2n = 44)、P. somniferum (2n = 22)、P. rhoeas (2n = 14) 和 P. bracteatum (2n = 14))生成了近乎完整的基因组组装,总共代表了 45 个无间隙中心粒。我们发现了四个中心粒卫星(cenSat)家族,并通过实验验证了其中两个代表。对于两个全多倍体基因组(P. somniferum 和 P. setigerum),我们描述了每个卫星的亚基因组分布,并确定了杂交后中心粒进化的 "同质化 "阶段。中心粒周围区域的种间比较进一步揭示了广泛的由中心粒介导的染色体重排。综合这些结果,我们提出了一个研究杂交后中心粒竞争的模型,并进一步揭示了中心粒在物种演化中的复杂作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
7.10
自引率
0.00%
发文量
0
期刊最新文献
A combined deep learning framework for mammalian m6A site prediction. Analysis of single-cell CRISPR perturbations indicates that enhancers predominantly act multiplicatively. Complex structural variation is prevalent and highly pathogenic in pediatric solid tumors. Gene regulatory network inference from CRISPR perturbations in primary CD4+ T cells elucidates the genomic basis of immune disease. Leveraging genomes to support conservation and bioeconomy policies in a megadiverse country.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1