The Effect of Oleuropein in AIF and MMP-9 in Traumatic Brain Injury Rat Model.

IF 2.5 Q3 CELL BIOLOGY Cellular Physiology and Biochemistry Pub Date : 2024-07-31 DOI:10.33594/000000717

Abdurrahman Mousa, Ridha Dharmajaya, Julia Reveny, Khairul Putra Surbakti, Hanif Gordang Tobing, Syafruddin Ilyas, Rosita Juwita Sembiring, Cut Aria Arina, Wibi Riawan

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Abstract

Background/aims: Traumatic brain injury is a significant public problem with an incidence of 10 million people per year, causing the largest deaths and disabilities worldwide. Head injuries can be classified into primary and secondary head injuries. Secondary head injuries can be caused by several factors such as ischemia, cerebral edema, and neuroinflammation. AIF and MMP-9 are two parameters that can be indicators in measuring the effect of Oleuropein on traumatic brain injury in rats. Oleuropein itself has many activities such as antioxidant, anti-apoptotic, antimicrobial, anti-inflammatory, and neuroprotective.

Methods: Adult male Sprague-Dawley rats (250-350 grams) were exposed to head injury, with or without intraperitoneal administration of Oleuropein. Within 24-72 hours brain tissue was isolated for immunohistochemical analysis, ELISA, and TUNEL. AIF, GFAP, MMP-9, and HMGB-1 levels were determined using immunohistochemistry in both the control and treatment groups. Statistical analysis was made using the One-Way Analysis of Variance (ANOVA) and paired t-test.

Results: The results showed that Oleuropein was able to reduce AIF and MMP-9 levels in rats with traumatic brain injury. This indicates that Oleuropein has a neuroprotective effect by reducing inflammation and apoptosis.

Conclusion: Oleuropein has a potential neuroprotective effect in traumatic brain injury by reducing inflammation and apoptosis. Therefore, Oleuropein can be considered as a potential therapeutic agent for traumatic brain injury in the future.

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欧叶素对创伤性脑损伤大鼠模型中 AIF 和 MMP-9 的影响

背景/目的：创伤性脑损伤是一个重大的公共问题，每年的发病率高达 1000 万人，是全世界造成死亡和残疾人数最多的疾病。头部创伤可分为原发性和继发性头部创伤。继发性头部损伤可由多种因素引起，如缺血、脑水肿和神经炎症。AIF和MMP-9是衡量欧叶素对大鼠脑外伤影响的两个指标。油菜素本身具有抗氧化、抗凋亡、抗菌、抗炎和神经保护等多种活性：方法：成年雄性 Sprague-Dawley 大鼠（250-350 克）头部受伤，腹腔注射或不注射欧叶素。在 24-72 小时内分离脑组织，进行免疫组化分析、ELISA 和 TUNEL 检测。对照组和治疗组的 AIF、GFAP、MMP-9 和 HMGB-1 水平均通过免疫组化法测定。统计分析采用单因素方差分析（ANOVA）和配对t检验：结果表明，欧来宁能够降低脑外伤大鼠的AIF和MMP-9水平。结论：油菜素具有潜在的神经保护作用：结论：油菜素通过减少炎症和细胞凋亡，对脑外伤具有潜在的神经保护作用。结论：油菜素通过减少炎症和细胞凋亡，对创伤性脑损伤具有潜在的神经保护作用，因此，油菜素可被视为未来治疗创伤性脑损伤的潜在药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cellular Physiology and Biochemistry 生物-生理学

CiteScore

5.80

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Cellular Physiology and Biochemistry is a multidisciplinary scientific forum dedicated to advancing the frontiers of basic cellular research. It addresses scientists from both the physiological and biochemical disciplines as well as related fields such as genetics, molecular biology, pathophysiology, pathobiochemistry and cellular toxicology & pharmacology. Original papers and reviews on the mechanisms of intracellular transmission, cellular metabolism, cell growth, differentiation and death, ion channels and carriers, and the maintenance, regulation and disturbances of cell volume are presented. Appearing monthly under peer review, Cellular Physiology and Biochemistry takes an active role in the concerted international effort to unravel the mechanisms of cellular function.