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Tendon Cell Biology: Effect of Mechanical Loading. 肌腱细胞生物学:机械加载的影响
IF 2.5 Q3 CELL BIOLOGY Pub Date : 2024-11-21 DOI: 10.33594/000000743
Mikołaj Stańczak, Bartłomiej Kacprzak, Piotr Gawda

Tendons play a crucial role in the musculoskeletal system, connecting muscles to bones and enabling efficient force transfer. However, they are prone to acute and chronic injuries, which, if not properly repaired, can significantly impair function. Tendinopathy, a prevalent condition affecting approximately 20% of musculoskeletal complaints, arises from an imbalance between micro-injury accumulation and repair processes. The extracellular matrix (ECM) of tendons is a hierarchical structure comprising collagen fibrils, proteoglycans, and glycoproteins that regulate organization, hydration, and mechanical properties. Mechanotransduction pathways, mediated by integrins and focal adhesion complexes, activate signaling cascades such as MAPK/ERK and PI3K/Akt, driving tenocyte gene expression and ECM remodeling. Adaptations to load involve region-specific remodeling, with tensile regions favoring aligned Type I collagen and compressive regions promoting proteoglycans like aggrecan. Stress shielding or reduced loading disrupts these pathways, leading to matrix disorganization and inflammation, predisposing tendons to degenerative changes. Insights into these molecular mechanisms inform rehabilitation strategies to enhance tendon repair and mitigate tendinopathy progression in both athletic and general populations.

肌腱在肌肉骨骼系统中起着至关重要的作用,它将肌肉与骨骼连接起来,实现有效的力量传递。然而,肌腱容易受到急性和慢性损伤,如果修复不当,会严重影响功能。腱鞘炎是一种常见病,约占肌肉骨骼疾病的 20%,它是由微损伤积累和修复过程之间的不平衡引起的。肌腱的细胞外基质(ECM)是一种分层结构,由胶原纤维、蛋白多糖和糖蛋白组成,可调节组织、水合作用和机械性能。由整合素和病灶粘附复合物介导的机械传导途径可激活 MAPK/ERK 和 PI3K/Akt 等信号级联,从而驱动腱细胞基因表达和 ECM 重塑。对负荷的适应涉及特定区域的重塑,拉伸区域有利于排列整齐的 I 型胶原蛋白,而压缩区域则促进蛋白多糖(如 aggrecan)的形成。应力屏蔽或减轻负荷会破坏这些途径,导致基质紊乱和炎症,使肌腱容易发生退行性变化。对这些分子机制的深入了解为制定康复策略提供了信息,以加强肌腱修复并减轻运动员和普通人群肌腱病的发展。
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引用次数: 0
Antibacterial Activity of Silver Nanoparticles Prepared from Camellia Sinensis Extracts in Multi-Drug Resistant Staphylococcus Aureus. 用山茶提取物制备的银纳米粒子对耐多药金黄色葡萄球菌的抗菌活性
IF 2.5 Q3 CELL BIOLOGY Pub Date : 2024-11-11 DOI: 10.33594/000000741
Hawazen Salih, Rami Altameemi, Ahmed Al-Azawi
<p><strong>Background/aims: </strong>The nano-method has been used as a technique for creating novel, non-traditional antimicrobial agents. This effective method for treating infectious diseases has many advantages over conventional antibiotics, including increased efficacy against species that have developed drug resistance, and the ability to circumvent the development of resistance that disrupts a number of biological pathways. As a result, the objective of this study was to synthesize and characterize silver nanoparticles using phenolic compounds obtained from <i>Camellia sinensis</i> . The nanoparticles were then used as antibacterial agents on the multidrug resistant <i>Staphylococcus aureus,</i> as well as<i>,</i> biofilm formation mechanism were also investigated.</p><p><strong>Methods: </strong>Ten isolates of <i>Staphylococcus aureus</i> were acquired from the labs of the University of Baghdad's Genetic Engineering and Biotechnology Institute. The VITEK-2 system was used to confirm the diagnosis. Aqueous and methanolic extracts of <i>Camellia sinensis</i> leaves were used to create silver nanoparticles and obtain CAgNPs, which were then characterized using Atomic Fluorescence Microscopy (AFM), X-ray diffractometer (XRD), and Zeta potential analyzers. The extracts were put through a series of assays, including High-performance liquid chromatography (HPLC), antibacterial activity assessments, and the microtiter plate method to determine the lowest inhibitory concentration (MIC) and antibiofilm formation.</p><p><strong>Results: </strong>Both aqueous and methanolic extracts containing silver nanoparticles included spherical nanoparticles that may be single or combined. The HPLC results showed the presence of two phenolic compounds (gallic acid and caffeine) by comparing their retention durations to those of the reference compounds. The results of the antibacterial activity of (CAgNPs) showed that the methanolic (CAgNPs) extract was more effective than the aqueous (CAgNPs) extract, producing inhibitory zones of 15.67 ± 0.58 and 20.33 ± 0.58 mm, at 375 and 750 ppm respectively, when compared to the aqueous (CAgNPs) extract, which produced inhibitory zones of 12.33 ± 0.58 and 15.67 ± 0.58 mm, respectively. The MIC result also showed that the CAgNPs methanolic extract was more effective than the CAgNPs aqueous extract. The MIC of the CAgNPs methanolic extract on <i>S. aureus</i> isolates were 11.718 and 23.43 µg/ml, while the MIC of the CAgNPs aqueous extract on all <i>S. aureus</i> isolates were 46.87 µg/ml except isolate No. 3 and 6 which was 11.718 and 93.75 µg/ml respectively. Additionally, The anti-biofilm in <i>S. aureus</i> was increased when CAgNPs methanolic extract were used compared with the CAgNPs aqueous extract, the CAgNPs methanolic extract inhibited 80%, 90% and 100% of the biofilm formation of <i>S. aureus</i> in 23.43, 46.87 and 93.75 µg/ml respectively, while the anti-biofilm activity of the CAgNPs aqueous extract on <i>S. au
背景/目的:纳米方法已被用作一种制造新型非传统抗菌剂的技术。与传统抗生素相比,这种治疗传染病的有效方法有许多优点,包括对已产生抗药性的物种有更强的疗效,以及能够规避破坏一些生物途径的抗药性的产生。因此,本研究的目的是利用从山茶中提取的酚类化合物合成银纳米粒子并确定其特性。然后将纳米粒子作为抗菌剂,用于耐多药的金黄色葡萄球菌,同时还研究了生物膜的形成机制:从巴格达大学遗传工程和生物技术研究所实验室获得 10 株金黄色葡萄球菌分离株。使用 VITEK-2 系统进行确诊。用山茶叶的水提取物和甲醇提取物制造银纳米粒子并获得 CAgNPs,然后用原子荧光显微镜 (AFM)、X 射线衍射仪 (XRD) 和 Zeta 电位分析仪对其进行表征。对提取物进行了一系列检测,包括高效液相色谱法(HPLC)、抗菌活性评估和微孔板法,以确定最低抑菌浓度(MIC)和抗生物膜形成:结果:含有银纳米颗粒的水提取物和甲醇提取物都含有球形纳米颗粒,可能是单一的,也可能是组合的。通过比较两种酚类化合物(没食子酸和咖啡因)的保留时间与参考化合物的保留时间,高效液相色谱结果显示了这两种酚类化合物的存在。CAgNPs 的抗菌活性结果表明,甲醇(CAgNPs)提取物比水(CAgNPs)提取物更有效,在 375 ppm 和 750 ppm 浓度下分别产生 15.67 ± 0.58 毫米和 20.33 ± 0.58 毫米的抑菌区,而水(CAgNPs)提取物产生的抑菌区分别为 12.33 ± 0.58 毫米和 15.67 ± 0.58 毫米。MIC 结果也表明,CAgNPs 甲醇提取物比 CAgNPs 水提取物更有效。CAgNPs 甲醇提取物对金黄色葡萄球菌分离物的 MIC 值分别为 11.718 和 23.43 µg/ml,而 CAgNPs 水提取物对所有金黄色葡萄球菌分离物的 MIC 值均为 46.87 µg/ml,只有 3 号和 6 号分离物的 MIC 值分别为 11.718 和 93.75 µg/ml。此外,与 CAgNPs 水提取物相比,CAgNPs 甲醇提取物对金黄色葡萄球菌生物膜形成的抑制作用更强。CAgNPs甲醇提取物对金黄色葡萄球菌生物膜形成的抑制率分别为80%、90%和100%(浓度分别为23.43、46.87和93.75 µg/ml),而CAgNPs水提取物对金黄色葡萄球菌分离株生物膜形成的抑制率分别为80%和100%(浓度分别为46.87和93.75 µg/ml):山茶叶的甲醇提取物和水提取物是生产 CAgNPs 的成功方法。根据浓度的不同,合成的 CAgNPs 对金黄色葡萄球菌也具有显著的抗菌活性,并能抑制金黄色葡萄球菌生物膜的形成。
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引用次数: 0
Retraction. 撤回。
IF 2.5 Q3 CELL BIOLOGY Pub Date : 2024-10-31 DOI: 10.33594/000000739
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引用次数: 0
Retraction. 撤回。
IF 2.5 Q3 CELL BIOLOGY Pub Date : 2024-10-31 DOI: 10.33594/000000736
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引用次数: 0
Retraction. 撤回。
IF 2.5 Q3 CELL BIOLOGY Pub Date : 2024-10-31 DOI: 10.33594/000000740
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引用次数: 0
Retraction. 撤回。
IF 2.5 Q3 CELL BIOLOGY Pub Date : 2024-10-31 DOI: 10.33594/000000738
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引用次数: 0
Retraction. 撤回。
IF 2.5 Q3 CELL BIOLOGY Pub Date : 2024-10-31 DOI: 10.33594/000000737
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引用次数: 0
A Comprehensive Pan-Cancer Analysis of the Mitochondrial Uncoupling Protein UCP2, with a Focus on Sex and Gender-Related Aspects. 线粒体解偶联蛋白 UCP2 的泛癌症综合分析,重点关注与性别相关的方面。
IF 2.5 Q3 CELL BIOLOGY Pub Date : 2024-10-27 DOI: 10.33594/000000735
Soha Sadeghi, Vanessa Checchetto, Tatiana Varanita

Background/aims: Mitochondrial uncoupling protein 2 (UCP2) plays a crucial role in regulating oxidative stress and cellular metabolism, positioning it as an important subject in oncological research. The involvement of UCP2 in cancer is complex and context-dependent, suggesting it as a potential therapeutic target. In this study, we aimed to perform a comprehensive pan-cancer analysis of UCP2, with a particular focus on gender-related malignancies such as breast (BRCA), prostate (PRAD), ovarian (OV), and testicular tumors (TGCT).

Methods: We analyzed UCP2 expression in The Cancer Genome Atlas (TCGA), examining correlations with prognosis, tumor mutational burden (TMB), microsatellite instability (MSI), immune cell infiltration, immune checkpoint genes, genes involved in steroidogenesis, sex hormone receptor genes, and drug sensitivity.

Results: Significant variability in UCP2 expression was observed across cancer types. UCP2 levels were elevated in BRCA and OV but reduced in PRAD and TGCT. High UCP2 expression was associated with a better prognosis in OV and poorer overall survival in PRAD. Furthermore, UCP2 correlated with TMB and MSI in OV, TGCT, and BRCA. UCP2 expression was also linked to immune cell infiltration, immune checkpoint genes, steroidogenic genes, and sex hormone receptor genes, with variable effects depending on cancer type and gender. Additionally, UCP2 also demonstrated sensitivity to specific anticancer drugs.

Conclusion: Our findings highlight the interplay between UCP2, immune and hormonal pathways, and drug response and reveal potential opportunities for new therapeutic combinations, especially in gender-related cancers.

背景/目的:线粒体解偶联蛋白 2(UCP2)在调节氧化应激和细胞新陈代谢方面发挥着至关重要的作用,因此成为肿瘤学研究的一个重要课题。UCP2 在癌症中的参与是复杂的,且与环境有关,这表明它是一个潜在的治疗靶点。在本研究中,我们旨在对 UCP2 进行全面的泛癌症分析,尤其关注与性别相关的恶性肿瘤,如乳腺癌(BRCA)、前列腺癌(PRAD)、卵巢癌(OV)和睾丸癌(TGCT):我们分析了癌症基因组图谱(TCGA)中 UCP2 的表达,研究了其与预后、肿瘤突变负荷(TMB)、微卫星不稳定性(MSI)、免疫细胞浸润、免疫检查点基因、参与类固醇生成的基因、性激素受体基因和药物敏感性的相关性:结果:不同癌症类型的 UCP2 表达存在显著差异。在 BRCA 和 OV 中 UCP2 水平升高,而在 PRAD 和 TGCT 中则降低。UCP2 的高表达与 OV 的较好预后和 PRAD 的较差总生存率相关。此外,UCP2 与 OV、TGCT 和 BRCA 中的 TMB 和 MSI 相关。UCP2 的表达还与免疫细胞浸润、免疫检查点基因、类固醇生成基因和性激素受体基因有关,其影响因癌症类型和性别而异。此外,UCP2 还表现出对特定抗癌药物的敏感性:我们的研究结果突显了 UCP2、免疫和激素通路与药物反应之间的相互作用,并揭示了新疗法组合的潜在机会,尤其是在与性别相关的癌症中。
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引用次数: 0
Nitric Oxide-Dependent Regulation of Oxygen-Related Processes in a Rat Model of Lead Neurotoxicity: Influence of the Hypoxia Resistance Factor. 一氧化氮对铅神经毒性大鼠模型中氧相关过程的依赖性调节:缺氧抵抗因子的影响。
IF 2.5 Q3 CELL BIOLOGY Pub Date : 2024-10-20 DOI: 10.33594/000000734
Natalia Kurhaluk, Piotr Kamiński, Oleksandr Lukash, Halina Tkaczenko
<p><strong>Background/aims: </strong>Lead exposure is known to induce oxidative stress and neurotoxicity. Nitric oxide (NO) plays an important role in modulating oxidative stress, with L-arginine as a precursor of NO and N<sup>ω</sup>-nitro-L-arginine (L-NNA) as an inhibitor of NO synthase, an enzyme that catalyses the production of nitric oxide (NO) from L-arginine.</p><p><strong>Methods: </strong>This study investigated the differential effects of L-arginine and L-NNA on markers of oxidative stress and biochemical changes in brain tissue from rats with different levels of resistance to hypoxia exposed to lead nitrate. Rats with either low or high resistance to hypoxia were exposed to lead nitrate (oral 3.6 mg lead nitrate/kg b.w. per day for 30 days) and treated with L-arginine (600 mg/kg b.w., i.p., 30 min before and after exposure to lead nitrate) or L-NNA (35 mg/kg b.w., i.p., 30 min before and after exposure to lead nitrate). Brain tissue samples were analysed for lipid peroxidation, oxidative modification of proteins, and activity of antioxidant enzymes, including superoxide dismutase, catalase, glutathione reductase, and peroxidase, and total antioxidant status (TAS). We also examined the biomarkers of biochemical pathways involving the activity of alanine and aspartate aminotransferases, succinate dehydrogenase (SDH), and α-ketoglutarate dehydrogenase (KGDH). In addition, the trend observed was supported by assessments of the acetylcholine levels and acetylcholinesterase activity (ACh-AChE system) in brain tissue.</p><p><strong>Results: </strong>In rats with low resistance to hypoxia, the L-arginine treatment significantly reduced lipid peroxidation and oxidative protein modification but increased antioxidant enzyme activity, suggesting a protective effect against lead-induced oxidative stress. Conversely, in rats with high resistance to hypoxia, L-NNA had a protective effect, reducing lead-induced oxidative damage and decreasing lipid peroxidation, whereas L-arginine exacerbated oxidative stress and impaired antioxidant defences. These findings were supported by corresponding changes in the acetylcholine-acetylcholinesterase system, reflecting the observed patterns of lead-induced oxidative stress and neurotoxicity. The study shows that L-arginine exerts a protective effect by reducing lead-induced oxidative damage via an improvement in TAS. Our study shows that lead nitrate exposure significantly increases ala-nine and aspartate aminotransferase activity in brain tissue, with L-arginine exacerbating and L-NNA reversing this effect. The lead nitrate exposure also affected the activities of SDH and KGDH, which are important for cellular energy production and hypoxia resistance, with L-arginine altering SDH activity depending on the level of resistance and L-NNA enhancing both SDH and KGDH activities. These trends were further validated by alterations in the ACh-AChE system, highlighting the differential role of NO-dependent mechanisms in
背景/目的:众所周知,铅暴露会诱发氧化应激和神经毒性。一氧化氮(NO)在调节氧化应激中起着重要作用,L-精氨酸是 NO 的前体,而 Nω-硝基-L-精氨酸(L-NNA)是 NO 合酶的抑制剂,NO 合酶是催化 L-精氨酸产生一氧化氮(NO)的酶:本研究调查了 L-精氨酸和 L-NNA 对不同耐缺氧程度的大鼠脑组织氧化应激标记物和生化变化的不同影响。低耐受性或高耐受性大鼠暴露于硝酸铅(每天口服 3.6 毫克硝酸铅/千克体重,持续 30 天),并在暴露于硝酸铅前后 30 分钟接受 L-精氨酸(600 毫克/千克体重,静注)或 L-NNA(35 毫克/千克体重,静注,在暴露于硝酸铅前后 30 分钟)治疗。对脑组织样本进行了脂质过氧化、蛋白质氧化修饰、抗氧化酶(包括超氧化物歧化酶、过氧化氢酶、谷胱甘肽还原酶和过氧化物酶)活性和总抗氧化状态(TAS)分析。我们还检测了涉及丙氨酸和天冬氨酸氨基转移酶、琥珀酸脱氢酶(SDH)和α-酮戊二酸脱氢酶(KGDH)活性的生化途径生物标志物。此外,对脑组织中乙酰胆碱水平和乙酰胆碱酯酶活性(ACh-AChE 系统)的评估也支持所观察到的趋势:结果:在耐缺氧能力低的大鼠中,L-精氨酸处理能显著降低脂质过氧化和氧化蛋白质修饰,但能提高抗氧化酶的活性,这表明它对铅诱导的氧化应激具有保护作用。相反,在耐缺氧能力较强的大鼠中,L-NNA 具有保护作用,可减少铅诱导的氧化损伤并降低脂质过氧化,而 L-精氨酸则会加剧氧化应激并损害抗氧化防御能力。这些发现得到了乙酰胆碱-乙酰胆碱酯酶系统相应变化的支持,反映了观察到的铅诱导氧化应激和神经毒性模式。研究表明,L-精氨酸通过改善 TAS,减少铅诱导的氧化损伤,从而发挥保护作用。我们的研究表明,接触硝酸铅会显著增加脑组织中的丙氨酸氨基转移酶和天冬氨酸氨基转移酶活性,L-精氨酸会加剧这种效应,而 L-NNA 则会逆转这种效应。硝酸铅暴露还影响了 SDH 和 KGDH 的活性,而这两种物质对细胞能量生产和耐缺氧非常重要,L-精氨酸会根据耐缺氧程度改变 SDH 的活性,而 L-NNA 则会增强 SDH 和 KGDH 的活性。ACh-AChE 系统的改变进一步验证了这些趋势,凸显了 NO 依赖性机制在根据缺氧抵抗性调节铅诱导的神经毒性中的不同作用:这些发现提出了基于氧化应激特征的潜在靶向治疗策略,并强调了一氧化氮系统调节剂在抗衡铅诱导的生化改变和 ACh-AChE 系统动态方面的潜力,这取决于生物体的个体生理反应性。
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引用次数: 0
BMI-1 in Breast Cancer - Biological Role and Clinical Implications. 乳腺癌中的 BMI-1 - 生物学作用和临床意义。
IF 2.5 Q3 CELL BIOLOGY Pub Date : 2024-10-15 DOI: 10.33594/000000733
Aleksandra Szustka, Anna Krześlak

Breast cancer is the most frequent cancer among women. Despite extensive research in recent years the molecular basis of breast cancer development, growth and metastasis remains unclear. Numerous studies highlight the involvement of BMI-1 in tumorigenesis. BMI-1 protein is a key component of Polycomb Repressive Complex 1, which by ubiquitinylation of histone H2A, regulates expression of genes involved in various cellular processes including cell cycle, proliferation and programmed cell death. Overexpression of BMI-1 has been often associated with breast cancer development and progression. This review summarizes the current state of knowledge of BMI-1's role in breast cancer biology and its potential significance as prognostic marker and potential target of new anticancer therapy.

乳腺癌是女性最常见的癌症。尽管近年来进行了大量研究,但乳腺癌发生、生长和转移的分子基础仍不清楚。大量研究强调了 BMI-1 在肿瘤发生过程中的参与作用。BMI-1 蛋白是多聚核抑制复合体 1 的一个重要组成部分,它通过泛素化组蛋白 H2A,调节参与细胞周期、增殖和细胞程序性死亡等各种细胞过程的基因的表达。BMI-1 的过表达往往与乳腺癌的发生和发展有关。本综述总结了 BMI-1 在乳腺癌生物学中的作用及其作为预后标志物和新抗癌疗法潜在靶点的潜在意义。
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引用次数: 0
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Cellular Physiology and Biochemistry
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