{"title":"Comprehensive DNA metabarcoding-based detection of non-indigenous invertebrates in recreational marinas through a multi-substrate approach","authors":"","doi":"10.1016/j.marenvres.2024.106660","DOIUrl":null,"url":null,"abstract":"<div><p>eDNA metabarcoding has been increasingly employed in the monitoring of marine invertebrate non-indigenous species (NIS), in particular using filtered seawater. However, comprehensive detection of all NIS may require a diversity of sampling substrates. To assess the effectiveness of 5 sample types (hard and artificial substrates, water, zooplankton) on the recovery of invertebrates’ diversity, two marinas were monitored over three time points, using COI and 18S rRNA genes as DNA metabarcoding markers. We detected a total of 628 species and 23 NIS, with only up to 9% species and 17% of NIS detected by all sample types. Hard and artificial substrates were similar to each other but displayed the most significant difference in invertebrate recovery when compared to water eDNA and zooplankton. Five NIS are potential first records for Portugal. No NIS were detected in all sample types and seasons, highlighting the need for varied sampling approaches, and consideration of temporal variation for comprehensive marine NIS surveillance.</p></div>","PeriodicalId":18204,"journal":{"name":"Marine environmental research","volume":null,"pages":null},"PeriodicalIF":3.0000,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0141113624003210/pdfft?md5=eeaf036b1c510c06cd5daf090d469083&pid=1-s2.0-S0141113624003210-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Marine environmental research","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0141113624003210","RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
eDNA metabarcoding has been increasingly employed in the monitoring of marine invertebrate non-indigenous species (NIS), in particular using filtered seawater. However, comprehensive detection of all NIS may require a diversity of sampling substrates. To assess the effectiveness of 5 sample types (hard and artificial substrates, water, zooplankton) on the recovery of invertebrates’ diversity, two marinas were monitored over three time points, using COI and 18S rRNA genes as DNA metabarcoding markers. We detected a total of 628 species and 23 NIS, with only up to 9% species and 17% of NIS detected by all sample types. Hard and artificial substrates were similar to each other but displayed the most significant difference in invertebrate recovery when compared to water eDNA and zooplankton. Five NIS are potential first records for Portugal. No NIS were detected in all sample types and seasons, highlighting the need for varied sampling approaches, and consideration of temporal variation for comprehensive marine NIS surveillance.
期刊介绍:
Marine Environmental Research publishes original research papers on chemical, physical, and biological interactions in the oceans and coastal waters. The journal serves as a forum for new information on biology, chemistry, and toxicology and syntheses that advance understanding of marine environmental processes.
Submission of multidisciplinary studies is encouraged. Studies that utilize experimental approaches to clarify the roles of anthropogenic and natural causes of changes in marine ecosystems are especially welcome, as are those studies that represent new developments of a theoretical or conceptual aspect of marine science. All papers published in this journal are reviewed by qualified peers prior to acceptance and publication. Examples of topics considered to be appropriate for the journal include, but are not limited to, the following:
– The extent, persistence, and consequences of change and the recovery from such change in natural marine systems
– The biochemical, physiological, and ecological consequences of contaminants to marine organisms and ecosystems
– The biogeochemistry of naturally occurring and anthropogenic substances
– Models that describe and predict the above processes
– Monitoring studies, to the extent that their results provide new information on functional processes
– Methodological papers describing improved quantitative techniques for the marine sciences.