Pub Date : 2024-10-03DOI: 10.1016/j.marenvres.2024.106777
Yangyuan Zhou, Guosheng Zhang, Dawei Zhang, Ningzheng Zhu, Jinpei Bo, Xiangzhou Meng, Yao Chen, Yu Qin, Huajie Liu, Weiying Li
As emerging pollutants, microplastics can aggregate microorganisms on their surfaces and form biofilms, enriching antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs). Consequently, microplastic biofilms have become a focal point of research. Horizontal gene transfer is one of the primary mechanisms by which bacteria acquire antibiotic resistance, with much of the research focusing on suspended bacteria. However, microplastic biofilms, as hotspots for horizontal gene transfer, also merit significant investigation. This study primarily explored and compared the frequency of ARG conjugative transfer between suspended bacteria and microplastic biofilms. The results demonstrated that, compared to suspended bacteria, microplastic biofilms enhanced the frequency of ARG conjugative transfer by 7.2-19.6 times. Among them, biofilms on polyethylene microplastics showed the strongest promotion of conjugation. After the formation of microplastic biofilms, there was a significant increase in bacterial density within the biofilms, which raised the collision frequency of donor and recipient bacteria. Then microplastic biofilms facilitated the gene expression levels of outer membrane proteins, enhanced bacterial gene transfer capabilities, promoted the synthesis of conjugative pili, accelerated the formation of conjugative pairing systems, and elevated the expression levels of genes related to DNA replication and transfer systems, thereby enhancing the conjugative transfer of ARGs within microplastic biofilms. Among different types of microplastic biofilms, polyethylene biofilms exhibited the highest bacterial density, thus showing the highest frequency of ARG conjugation. This study highlights the risks associated with ARG conjugative transfer following the formation of microplastic biofilms and provides insights into the risks of microplastic and antibiotic resistance propagation in estuarine environments.
{"title":"Microplastic biofilms promote the horizontal transfer of antibiotic resistance genes in estuarine environments.","authors":"Yangyuan Zhou, Guosheng Zhang, Dawei Zhang, Ningzheng Zhu, Jinpei Bo, Xiangzhou Meng, Yao Chen, Yu Qin, Huajie Liu, Weiying Li","doi":"10.1016/j.marenvres.2024.106777","DOIUrl":"https://doi.org/10.1016/j.marenvres.2024.106777","url":null,"abstract":"<p><p>As emerging pollutants, microplastics can aggregate microorganisms on their surfaces and form biofilms, enriching antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs). Consequently, microplastic biofilms have become a focal point of research. Horizontal gene transfer is one of the primary mechanisms by which bacteria acquire antibiotic resistance, with much of the research focusing on suspended bacteria. However, microplastic biofilms, as hotspots for horizontal gene transfer, also merit significant investigation. This study primarily explored and compared the frequency of ARG conjugative transfer between suspended bacteria and microplastic biofilms. The results demonstrated that, compared to suspended bacteria, microplastic biofilms enhanced the frequency of ARG conjugative transfer by 7.2-19.6 times. Among them, biofilms on polyethylene microplastics showed the strongest promotion of conjugation. After the formation of microplastic biofilms, there was a significant increase in bacterial density within the biofilms, which raised the collision frequency of donor and recipient bacteria. Then microplastic biofilms facilitated the gene expression levels of outer membrane proteins, enhanced bacterial gene transfer capabilities, promoted the synthesis of conjugative pili, accelerated the formation of conjugative pairing systems, and elevated the expression levels of genes related to DNA replication and transfer systems, thereby enhancing the conjugative transfer of ARGs within microplastic biofilms. Among different types of microplastic biofilms, polyethylene biofilms exhibited the highest bacterial density, thus showing the highest frequency of ARG conjugation. This study highlights the risks associated with ARG conjugative transfer following the formation of microplastic biofilms and provides insights into the risks of microplastic and antibiotic resistance propagation in estuarine environments.</p>","PeriodicalId":18204,"journal":{"name":"Marine environmental research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-01DOI: 10.1016/j.marenvres.2024.106775
David Mazurais, Victor Simon, Pauline Auffret, Alexandre Cormier, Alexandra Dauvé, Lauriane Madec, Baptiste Tanguy-Guillo, Nicolas Gayet, Elodie Fleury, Jérémy Le Luyer
In this study, we examined the effect of near future ocean acidification (OA) on the transcriptome of a sensory organ in contact with surrounding water, the tongue in adult European sea bass (Dicentrarchus labrax) by mean of RNAseq experiment. We acquired a total of 14.1 Mb quality-trimmed reads covering 18,703 expressed genes from the tongue of fish reared from two generations at actual (pH 8.0 condition) and predicted near-future seawater pH (pH 7.6 condition). Gene ontologies analyses of expressed genes support the evidence that the tongue exhibits biological processes related to the sensory system, tooth mineralization and immune defences among others. Our data revealed only 295 OA-induced regulated genes with 114 up- and 181 down-regulated by OA. Functions over-represented encompass processes involved in organic substance metabolic process, RNA metabolism and especially RNA methylation which, combined with the regulation of some hsp genes expression, suggest a molecular response to stress which might contribute to lingual cell homeostasis under OA. The immune system process is also found enriched within OA-induced regulated genes. With the exception of one fatty acid receptor, known taste perception effectors were not impacted by OA in the tongue. However, a complementary droplet digital PCR approach dedicated to genes involved in gustatory signal transduction revealed the down regulation by OA of pyrimidinergic receptor (p2ry4) transcript expression in the gills of the fish. Combined with scanning electron microscopy analysis, our RNAseq data revealed that OA has no impact on processes related to teeth development and mineralization. Altogether, our data reveal that multigenerational exposure to OA has not a substantially effect on the tongue transcriptome but emphasis should be placed on investigating the potential physiological consequences related to the regulation of genes related to cell stress, immune system and fatty acid sensitivity to conclude on species resilience in face of OA.
在这项研究中,我们通过RNAseq实验研究了近期海洋酸化(OA)对欧洲鲈鱼(Dicentrarchus labrax)成鱼舌头这一与周围水接触的感觉器官转录组的影响。我们从实际海水 pH 值(pH 8.0 条件下)和预测的近未来海水 pH 值(pH 7.6 条件下)条件下饲养的两代鱼的舌头上获得了总计 14.1 Mb 的高质量修剪读数,涵盖 18,703 个表达基因。对表达基因的基因本体分析证明,鱼舌的生物过程与感觉系统、牙齿矿化和免疫防御等有关。我们的数据只发现了 295 个 OA 诱导的调控基因,其中 114 个上调,181 个下调。被高调的功能包括有机物质代谢过程、RNA 代谢过程,尤其是 RNA 甲基化过程,这些过程与一些 hsp 基因的表达调控结合在一起,表明在 OA 的作用下,分子对压力的反应可能有助于舌细胞的平衡。在 OA 诱导的调控基因中也发现了免疫系统过程。除一种脂肪酸受体外,已知的味觉感知效应因子并未受到舌头 OA 的影响。然而,一种专门针对味觉信号转导相关基因的互补液滴数字 PCR 方法显示,OA 下调了鱼鳃中嘧啶能受体(p2ry4)转录本的表达。结合扫描电子显微镜分析,我们的 RNAseq 数据显示,OA 对牙齿发育和矿化过程没有影响。总之,我们的数据显示,多代暴露于 OA 不会对舌头转录组产生实质性影响,但应重点研究与细胞应激、免疫系统和脂肪酸敏感性相关基因的调控有关的潜在生理后果,从而得出物种面对 OA 的恢复能力的结论。
{"title":"Mutligenerational chronic exposure to near future ocean acidification in European sea bass (Dicentrarchus labrax): Insights into the regulation of the transcriptome in a sensory organ involved in feed intake, the tongue.","authors":"David Mazurais, Victor Simon, Pauline Auffret, Alexandre Cormier, Alexandra Dauvé, Lauriane Madec, Baptiste Tanguy-Guillo, Nicolas Gayet, Elodie Fleury, Jérémy Le Luyer","doi":"10.1016/j.marenvres.2024.106775","DOIUrl":"https://doi.org/10.1016/j.marenvres.2024.106775","url":null,"abstract":"<p><p>In this study, we examined the effect of near future ocean acidification (OA) on the transcriptome of a sensory organ in contact with surrounding water, the tongue in adult European sea bass (Dicentrarchus labrax) by mean of RNAseq experiment. We acquired a total of 14.1 Mb quality-trimmed reads covering 18,703 expressed genes from the tongue of fish reared from two generations at actual (pH 8.0 condition) and predicted near-future seawater pH (pH 7.6 condition). Gene ontologies analyses of expressed genes support the evidence that the tongue exhibits biological processes related to the sensory system, tooth mineralization and immune defences among others. Our data revealed only 295 OA-induced regulated genes with 114 up- and 181 down-regulated by OA. Functions over-represented encompass processes involved in organic substance metabolic process, RNA metabolism and especially RNA methylation which, combined with the regulation of some hsp genes expression, suggest a molecular response to stress which might contribute to lingual cell homeostasis under OA. The immune system process is also found enriched within OA-induced regulated genes. With the exception of one fatty acid receptor, known taste perception effectors were not impacted by OA in the tongue. However, a complementary droplet digital PCR approach dedicated to genes involved in gustatory signal transduction revealed the down regulation by OA of pyrimidinergic receptor (p2ry4) transcript expression in the gills of the fish. Combined with scanning electron microscopy analysis, our RNAseq data revealed that OA has no impact on processes related to teeth development and mineralization. Altogether, our data reveal that multigenerational exposure to OA has not a substantially effect on the tongue transcriptome but emphasis should be placed on investigating the potential physiological consequences related to the regulation of genes related to cell stress, immune system and fatty acid sensitivity to conclude on species resilience in face of OA.</p>","PeriodicalId":18204,"journal":{"name":"Marine environmental research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-30DOI: 10.1016/j.marenvres.2024.106773
James W Sturges, Jeremy T Claisse
To restore an area of temperate rocky-reef degraded by sedimentation, scour, and burial, a large quarry rock reef, the Palos Verdes Restoration Reef (PVR), was built with a heterogenous design including high relief elements intended to increase fish biomass productivity and support a diverse reef community. The replicated design features provide a unique opportunity to study the effects of reef design on fish habitat use patterns. To determine how submodule scale habitat features are associated with variation in the assemblage structure of eleven focal fish species on the PVR we conducted diver-operated stereo-video surveys on all 18 PVR modules 9-13 months after construction. The highest mean densities of most focal fish species and highest total fish densities were observed on high and medium-relief reef submodules and their adjacent ecotones positioned on the offshore sides or ends of modules. These included the most abundant species on the PVR, the zooplanktivorous Blacksmith (Chromis punctipinnis), as well as the fishery species Kelp Bass (Paralabrax clathratus) and California Sheephead (Bodianus pulcher). On the inshore side of parallel modules, the reef and ecotone transects on low and medium relief submodules exhibited the lowest total mean fish densities, and consistently lower mean focal fish species densities. Focal fish species assemblages also differed between the reef and sand-rock ecotone transects. Reef-resident planktivorous fishes likely contribute to reef primary and secondary productivity through consumer mediated nutrient transport and are an important consideration in restoration reef design. Future reef restoration designs should consider incorporating replicated heterogeneous design features including the placement of higher relief elements relative to shore and current patterns as a special consideration for providing habitat for planktivorous reef-resident fishes.
{"title":"Quarry rock reef design features influence fish assemblage structure across a systematically heterogenous restoration reef.","authors":"James W Sturges, Jeremy T Claisse","doi":"10.1016/j.marenvres.2024.106773","DOIUrl":"https://doi.org/10.1016/j.marenvres.2024.106773","url":null,"abstract":"<p><p>To restore an area of temperate rocky-reef degraded by sedimentation, scour, and burial, a large quarry rock reef, the Palos Verdes Restoration Reef (PVR), was built with a heterogenous design including high relief elements intended to increase fish biomass productivity and support a diverse reef community. The replicated design features provide a unique opportunity to study the effects of reef design on fish habitat use patterns. To determine how submodule scale habitat features are associated with variation in the assemblage structure of eleven focal fish species on the PVR we conducted diver-operated stereo-video surveys on all 18 PVR modules 9-13 months after construction. The highest mean densities of most focal fish species and highest total fish densities were observed on high and medium-relief reef submodules and their adjacent ecotones positioned on the offshore sides or ends of modules. These included the most abundant species on the PVR, the zooplanktivorous Blacksmith (Chromis punctipinnis), as well as the fishery species Kelp Bass (Paralabrax clathratus) and California Sheephead (Bodianus pulcher). On the inshore side of parallel modules, the reef and ecotone transects on low and medium relief submodules exhibited the lowest total mean fish densities, and consistently lower mean focal fish species densities. Focal fish species assemblages also differed between the reef and sand-rock ecotone transects. Reef-resident planktivorous fishes likely contribute to reef primary and secondary productivity through consumer mediated nutrient transport and are an important consideration in restoration reef design. Future reef restoration designs should consider incorporating replicated heterogeneous design features including the placement of higher relief elements relative to shore and current patterns as a special consideration for providing habitat for planktivorous reef-resident fishes.</p>","PeriodicalId":18204,"journal":{"name":"Marine environmental research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-27DOI: 10.1016/j.marenvres.2024.106765
Jamille da Silva Rabelo, Fátima Cristiane Teles de Carvalho, Rosa Helena Rebouças, Oscarina Viana de Sousa
All over the world, the oceans are the final destination of sewage transported by river estuaries, rainwater and other coastal discharges. The risks to human health related to direct contact with water and consumption of contaminated fish are well known, but little is known about the potential for atmospheric exposure to pollutants and pathogens from contaminated seawater. The release of microbial particles from the sea into the atmosphere occurs mainly by the eruption of rising bubbles through the sea surface microlayer (SML) or by sea spray. We investigated the heterotrophic bacteria density and relative abundance in SML and bioaerosols originated on the seafront of Fortaleza (Atlantic coastal zone, northeastern Brazil) influenced by wastewater disposal. There was a difference in the density of total heterotrophic bacteria (THB) according to the matrix analyzed during two seasons: the bacterial count was highest in the SML during the rainy season while the highest number of bacteria in bioaerosols samples was recorded during the dry season. Twenty-nine bacterial taxonomic groups were identified with variable abundance for both environments. These were the same in both matrices, with environmental variables influencing their abundance and composition. The contribution of the marine and continental environments in shaping the microbiota of the SML and coastal bioaerosols was clear, with the constant and representative presence of Enterobacteria standing out. The aerosolization of bacteria resulting from the discharge of untreated sewage is an important issue related to coastal environmental health and ecological safety.
{"title":"Microbial ocean-atmosphere transfer: The influence of sewage discharge into coastal waters on bioaerosols from an urban beach in the subtropical Atlantic.","authors":"Jamille da Silva Rabelo, Fátima Cristiane Teles de Carvalho, Rosa Helena Rebouças, Oscarina Viana de Sousa","doi":"10.1016/j.marenvres.2024.106765","DOIUrl":"https://doi.org/10.1016/j.marenvres.2024.106765","url":null,"abstract":"<p><p>All over the world, the oceans are the final destination of sewage transported by river estuaries, rainwater and other coastal discharges. The risks to human health related to direct contact with water and consumption of contaminated fish are well known, but little is known about the potential for atmospheric exposure to pollutants and pathogens from contaminated seawater. The release of microbial particles from the sea into the atmosphere occurs mainly by the eruption of rising bubbles through the sea surface microlayer (SML) or by sea spray. We investigated the heterotrophic bacteria density and relative abundance in SML and bioaerosols originated on the seafront of Fortaleza (Atlantic coastal zone, northeastern Brazil) influenced by wastewater disposal. There was a difference in the density of total heterotrophic bacteria (THB) according to the matrix analyzed during two seasons: the bacterial count was highest in the SML during the rainy season while the highest number of bacteria in bioaerosols samples was recorded during the dry season. Twenty-nine bacterial taxonomic groups were identified with variable abundance for both environments. These were the same in both matrices, with environmental variables influencing their abundance and composition. The contribution of the marine and continental environments in shaping the microbiota of the SML and coastal bioaerosols was clear, with the constant and representative presence of Enterobacteria standing out. The aerosolization of bacteria resulting from the discharge of untreated sewage is an important issue related to coastal environmental health and ecological safety.</p>","PeriodicalId":18204,"journal":{"name":"Marine environmental research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142365770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-26DOI: 10.1016/j.marenvres.2024.106771
Ocean warming and acidification driven by anthropogenic CO2 emissions may impact the mineral composition of marine calcifiers. Species with high skeletal Mg content could be more susceptible in polar regions due to the increased solubility of CO2 at lower temperatures. We aimed to assess the environmental influence on skeletal Mg content of Antarctic echinoderms belonging to Asteroidea, Ophiuroidea, Echinoidea and Holothuroidea classes, along a latitudinal gradient from the South Shetland Islands to Rothera (Adelaide Island). We found that all skeletal structures, except for echinoid spines, exhibited high Mg content, with asteroids showing the highest levels. Our results suggest that asteroids and holothuroids exert a higher biological capacity to regulate Mg incorporation into their skeletons. In contrast, the variability observed in the skeletal Mg content of ophiuroids and echinoids appears to be more influenced by local environmental conditions. Species-specific differences in how environmental factors affect the skeletal Mg content can thus be expected as a response to global climate change.
{"title":"Skeletal magnesium content in Antarctic echinoderms along a latitudinal gradient","authors":"","doi":"10.1016/j.marenvres.2024.106771","DOIUrl":"10.1016/j.marenvres.2024.106771","url":null,"abstract":"<div><div>Ocean warming and acidification driven by anthropogenic CO<sub>2</sub> emissions may impact the mineral composition of marine calcifiers. Species with high skeletal Mg content could be more susceptible in polar regions due to the increased solubility of CO<sub>2</sub> at lower temperatures. We aimed to assess the environmental influence on skeletal Mg content of Antarctic echinoderms belonging to Asteroidea, Ophiuroidea, Echinoidea and Holothuroidea classes, along a latitudinal gradient from the South Shetland Islands to Rothera (Adelaide Island). We found that all skeletal structures, except for echinoid spines, exhibited high Mg content, with asteroids showing the highest levels. Our results suggest that asteroids and holothuroids exert a higher biological capacity to regulate Mg incorporation into their skeletons. In contrast, the variability observed in the skeletal Mg content of ophiuroids and echinoids appears to be more influenced by local environmental conditions. Species-specific differences in how environmental factors affect the skeletal Mg content can thus be expected as a response to global climate change.</div></div>","PeriodicalId":18204,"journal":{"name":"Marine environmental research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-26DOI: 10.1016/j.marenvres.2024.106768
Gehan M El Zokm, Ghada F El-Said, Naglaa F Soliman
This study is the first of its kind in terms of focusing on the seasonal monitoring of bromine species (bromide- and bromate) and some of the main physicochemical parameters in the surface water of stations inside and in front of the El Noubareya and El-Umum drains that flow directly or indirectly to the Egyptian Mediterranean coast at A (El Noubareya Drain) and B (El-Mex Bay) sites. Among the bromine species, bromate (BrO3-) is a disinfection byproduct considered by many international agencies to have a potential carcinogenic effect in humans and is also known to be ecologically toxic to aquatic organisms. Drain water samples collected from the studied sites A and B had a bromide/chlorinity ratio (3.85E-03 - 6.25E-03 and 3.27E-03 - 6.97E-03, respectively) significantly higher than the typical value for open seawater (3.50E-03), showing significant dilution with wastewater at drain stations in the investigated sites. The source and origin of bromine species and the major ions studied associated with the wastewater units were identified and tracked by calculating the ion/chlorinity ratio and multivariate analysis. The total hazard quotient (THQ) for bromate intake and dermal exposure in children, females, and males demonstrates negligible harm to human health. The toxic unit (TU) and the sum of toxic units (STU) values of the three trophic levels in the surface water for the two sites under investigation yielded approximately comparable values for risk quotient (RQ) and mixture risk characterization ratios (RCRmix(MEC/PNEC)), indicating that invertebrates are more sensitive to bromate dangers than fish and algae. The study highlights the importance of conducting large-scale laboratory tests on the effluents resulting from wastewater treatment units, including bromide levels, to prevent the formation of dangerous side compounds such as bromate, which may have negative effects on populations and may lead to the toxicity of trophic levels in ecosystems.
这项研究是首次对直接或间接流向埃及地中海沿岸的 El Noubareya 和 El-Umum 排水渠 A(El Noubareya 排水渠)和 B(El-Mex 海湾)站点内和站点前的地表水中的溴物种(溴化物和溴酸盐)和一些主要物理化学参数进行季节性监测。在溴的种类中,溴酸盐(BrO3-)是一种消毒副产品,许多国际机构认为它对人类有潜在的致癌作用,而且已知它对水生生物具有生态毒性。从研究地点 A 和 B 采集的排水样本的溴/氯比值(分别为 3.85E-03 - 6.25E-03 和 3.27E-03 - 6.97E-03)明显高于开放式海水的典型值(3.50E-03),表明调查地点排水站的排水样本被废水严重稀释。通过计算离子/氯度比值和多元分析,确定并追踪了与废水单元相关的溴物种和主要离子的来源和出处。儿童、女性和男性摄入和皮肤接触溴酸盐的总危害商数(THQ)表明,对人体健康的危害可以忽略不计。调查的两个地点地表水中三个营养级的毒性单位(TU)和毒性单位总和(STU)值得出了大致相当的风险商数(RQ)和混合物风险特征比(RCRmix(MEC/PNEC))值,表明无脊椎动物比鱼类和藻类对溴酸盐危害更敏感。这项研究强调了对废水处理装置产生的污水(包括溴化物含量)进行大规模实验室检测的重要性,以防止形成溴酸盐等危险的副化合物,这些副化合物可能会对种群产生负面影响,并可能导致生态系统中营养级的毒性。
{"title":"Ecotoxicity of bromate and human health risks resulting from wastewater treatment units' effluents associated with some key physicochemical parameters in two hotspots connected to the Egyptian Mediterranean Sea.","authors":"Gehan M El Zokm, Ghada F El-Said, Naglaa F Soliman","doi":"10.1016/j.marenvres.2024.106768","DOIUrl":"https://doi.org/10.1016/j.marenvres.2024.106768","url":null,"abstract":"<p><p>This study is the first of its kind in terms of focusing on the seasonal monitoring of bromine species (bromide<sup>-</sup> and bromate) and some of the main physicochemical parameters in the surface water of stations inside and in front of the El Noubareya and El-Umum drains that flow directly or indirectly to the Egyptian Mediterranean coast at A (El Noubareya Drain) and B (El-Mex Bay) sites. Among the bromine species, bromate (BrO<sub>3</sub><sup>-</sup>) is a disinfection byproduct considered by many international agencies to have a potential carcinogenic effect in humans and is also known to be ecologically toxic to aquatic organisms. Drain water samples collected from the studied sites A and B had a bromide/chlorinity ratio (3.85E-03 - 6.25E-03 and 3.27E-03 - 6.97E-03, respectively) significantly higher than the typical value for open seawater (3.50E-03), showing significant dilution with wastewater at drain stations in the investigated sites. The source and origin of bromine species and the major ions studied associated with the wastewater units were identified and tracked by calculating the ion/chlorinity ratio and multivariate analysis. The total hazard quotient (THQ) for bromate intake and dermal exposure in children, females, and males demonstrates negligible harm to human health. The toxic unit (TU) and the sum of toxic units (STU) values of the three trophic levels in the surface water for the two sites under investigation yielded approximately comparable values for risk quotient (RQ) and mixture risk characterization ratios (RCR<sub>mix(MEC/PNEC)</sub>), indicating that invertebrates are more sensitive to bromate dangers than fish and algae. The study highlights the importance of conducting large-scale laboratory tests on the effluents resulting from wastewater treatment units, including bromide levels, to prevent the formation of dangerous side compounds such as bromate, which may have negative effects on populations and may lead to the toxicity of trophic levels in ecosystems.</p>","PeriodicalId":18204,"journal":{"name":"Marine environmental research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-25DOI: 10.1016/j.marenvres.2024.106769
Anamarija Kolda, Maja Mucko, Ana Rapljenović, Zrinka Ljubešić, Kristina Pikelj, Željko Kwokal, Hana Fajković, Vlado Cuculić
The coasts of the world's oceans and seas accumulate various types of floating debris, commonly known as beach wracks, including organic seaweeds, seagrass, and ubiquitous anthropogenic waste, mainly plastic. Beach wrack microbiome (MB), surviving in the form of a biofilm, ensures decomposition and remineralization of wracks, but can also serve as a vector of potential pathogens in the environment. Through the interdisciplinary approach and comprehensive sampling design that includes geological analysis of the sediment, plastic debris composition analysis (ATR-FTIR) and application of 16S rRNA gene metabarcoding of beach wrack MBs, this study aims to describe MB in relation to beach exposure, sediment type and plastic pollution. Major contributors in beach wrack MB were Proteobacteria, Bacteroidetes, Actinobacteria, Planctomycetes, Verrucomicrobia and Firmicutes and there was significant dissimilarity between sample groups with Vibrio, Cobetia and Planococcus shaping the Exposed beach sample group and Cyclobacteriaceae and Flavobacterium shaping the Sheltered beach sample group. Our results suggest plastisphere MB is mostly shaped by beach exposure, type of seagrass, sediment type and probably beach naturalness with heavy influence of seawater MB and shows no significant dissimilarity between MBs from a variety of microplastics (MP). Putative functional analysis of MB detected plastic degradation and potential human pathogen bacteria in both beach wrack and seawater MB. The research provides the next crucial step in beach wrack MP accumulation research, MB composition and functional investigation with focus on beach exposure as an important variable.
{"title":"Beach wracks microbiome and its putative function in plastic polluted Mediterranean marine ecosystem.","authors":"Anamarija Kolda, Maja Mucko, Ana Rapljenović, Zrinka Ljubešić, Kristina Pikelj, Željko Kwokal, Hana Fajković, Vlado Cuculić","doi":"10.1016/j.marenvres.2024.106769","DOIUrl":"https://doi.org/10.1016/j.marenvres.2024.106769","url":null,"abstract":"<p><p>The coasts of the world's oceans and seas accumulate various types of floating debris, commonly known as beach wracks, including organic seaweeds, seagrass, and ubiquitous anthropogenic waste, mainly plastic. Beach wrack microbiome (MB), surviving in the form of a biofilm, ensures decomposition and remineralization of wracks, but can also serve as a vector of potential pathogens in the environment. Through the interdisciplinary approach and comprehensive sampling design that includes geological analysis of the sediment, plastic debris composition analysis (ATR-FTIR) and application of 16S rRNA gene metabarcoding of beach wrack MBs, this study aims to describe MB in relation to beach exposure, sediment type and plastic pollution. Major contributors in beach wrack MB were Proteobacteria, Bacteroidetes, Actinobacteria, Planctomycetes, Verrucomicrobia and Firmicutes and there was significant dissimilarity between sample groups with Vibrio, Cobetia and Planococcus shaping the Exposed beach sample group and Cyclobacteriaceae and Flavobacterium shaping the Sheltered beach sample group. Our results suggest plastisphere MB is mostly shaped by beach exposure, type of seagrass, sediment type and probably beach naturalness with heavy influence of seawater MB and shows no significant dissimilarity between MBs from a variety of microplastics (MP). Putative functional analysis of MB detected plastic degradation and potential human pathogen bacteria in both beach wrack and seawater MB. The research provides the next crucial step in beach wrack MP accumulation research, MB composition and functional investigation with focus on beach exposure as an important variable.</p>","PeriodicalId":18204,"journal":{"name":"Marine environmental research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-24DOI: 10.1016/j.marenvres.2024.106764
Ostreococcus (Mamiellophyceae, Chlorophyta) is a cosmopolitan genus of marine pico-phytoplankton and the smallest free-living photosynthetic eukaryotes with cell size of 1–2 μm. To understand the diversity and spatio-temporal distribution of Ostreococcus in the Rongcheng coastal regions in northern China, metabarcoding analysis based on the 18S rDNA V4 molecular marker was applied to study the molecular diversity and seasonal dynamics of Ostreococcus in three typical mariculture bays (Rongcheng Bay, Lidao Bay and Sanggou Bay). A total of 103 amplicon sequence variants (ASVs) annotated as Ostreococcus were detected in these three typical mariculture bays throughout the year. The top five ASVs in terms of abundance were ASV4, ASV9, ASV14, ASV28 and ASV109, totally occupying 99.1% of Ostreococcus reads. Phylogenetic analysis showed that these five dominant ASVs represented two Ostreococcus ecotypes (OI and OII) and were grouped into four Ostreococcus clades including Ostreococcus lucimarinus (ASV9) and Ostreococcus tauri (ASV28 and ASV109) in OI, and Ostreococcus sp. RC1 (ASV4) and Ostreococcus sp. RC2 (ASV14) in OII, which provided direct evidence to support the co-existence of two ecotypes in the Rongcheng coastal regions. Five dominant ASVs in OI and OII exhibited two distinct seasonal distribution patterns. Three dominant ASVs (ASV9, ASV28 and ASV109) in OI could be detected in all four seasons of the year, exhibiting native distribution properties, while two ASVs (ASV4 and ASV14) in OII decreased sharply in winter and could not be detected in spring, exhibiting characteristics of alien inputs. The composition, succession and association of Ostreococcus community were mainly driven by water temperature in these mariculture bays. This study helps us systematically understand the molecular diversity and distribution patterns of Ostreococcus in typical mariculture bays in northern China, laying the foundation for understanding and revealing the ecological functions of pico-phytoplankton.
海洋球藻(Mamiellophyceae, Chlorophyta)是一种世界性的海洋微浮游植物属,也是最小的自由生活光合真核生物,细胞大小为1-2微米。为了解中国北方荣成沿海地区赤潮球藻的多样性和时空分布,应用基于 18S rDNA V4 分子标记的代谢编码分析方法,研究了三个典型海湾(荣成湾、里岛湾和桑沟湾)赤潮球藻的分子多样性和季节动态。在这三个典型海湾中,全年共检测到 103 个注释为 Ostreococcus 的扩增子序列变体(ASV)。丰度最高的五个 ASV 分别为 ASV4、ASV9、ASV14、ASV28 和 ASV109,共占 Ostreococcus 读数的 99.1%。系统发育分析表明,这五个优势 ASV 代表了两个 Ostreococcus 生态型(OI 和 OII),并被归入四个 Ostreococcus 支系,包括 OI 支系中的 Ostreococcus lucimarinus(ASV9)和 Ostreococcus tauri(ASV28 和 ASV109),以及 OI 支系中的 Ostreococcus sp.RC1(ASV4)和 RC2(ASV14),为荣成沿海地区两种生态型共存提供了直接证据。OI 和 OII 中的 5 种优势 ASV 表现出两种不同的季节分布模式。OI中的3个优势ASV(ASV9、ASV28和ASV109)在一年四季中均可检测到,表现出原生分布特征;而OII中的2个ASV(ASV4和ASV14)在冬季急剧减少,春季无法检测到,表现出外来输入特征。这些海水养殖海湾中尾孢球藻群落的组成、演替和关联主要受水温的影响。本研究有助于我们系统地了解中国北方典型海水养殖海湾中尾孢藻的分子多样性和分布模式,为认识和揭示微小浮游植物的生态功能奠定基础。
{"title":"Molecular diversity and seasonal dynamics of Ostreococcus (Mamiellophyceae, Chlorophyta) in typical mariculture bays based on metabarcoding analysis","authors":"","doi":"10.1016/j.marenvres.2024.106764","DOIUrl":"10.1016/j.marenvres.2024.106764","url":null,"abstract":"<div><div><em>Ostreococcus</em> (Mamiellophyceae, Chlorophyta) is a cosmopolitan genus of marine pico-phytoplankton and the smallest free-living photosynthetic eukaryotes with cell size of 1–2 μm. To understand the diversity and spatio-temporal distribution of <em>Ostreococcus</em> in the Rongcheng coastal regions in northern China, metabarcoding analysis based on the 18S rDNA V4 molecular marker was applied to study the molecular diversity and seasonal dynamics of <em>Ostreococcus</em> in three typical mariculture bays (Rongcheng Bay, Lidao Bay and Sanggou Bay). A total of 103 amplicon sequence variants (ASVs) annotated as <em>Ostreococcus</em> were detected in these three typical mariculture bays throughout the year. The top five ASVs in terms of abundance were ASV4, ASV9, ASV14, ASV28 and ASV109, totally occupying 99.1% of <em>Ostreococcus</em> reads. Phylogenetic analysis showed that these five dominant ASVs represented two <em>Ostreococcus</em> ecotypes (OI and OII) and were grouped into four <em>Ostreococcus</em> clades including <em>Ostreococcus lucimarinus</em> (ASV9) and <em>Ostreococcus tauri</em> (ASV28 and ASV109) in OI, and <em>Ostreococcus</em> sp. RC1 (ASV4) and <em>Ostreococcus</em> sp. RC2 (ASV14) in <span>OII</span>, which provided direct evidence to support the co-existence of two ecotypes in the Rongcheng coastal regions. Five dominant ASVs in OI and OII exhibited two distinct seasonal distribution patterns. Three dominant ASVs (ASV9, ASV28 and ASV109) in OI could be detected in all four seasons of the year, exhibiting native distribution properties, while two ASVs (ASV4 and ASV14) in OII decreased sharply in winter and could not be detected in spring, exhibiting characteristics of alien inputs. The composition, succession and association of <em>Ostreococcus</em> community were mainly driven by water temperature in these mariculture bays. This study helps us systematically understand the molecular diversity and distribution patterns of <em>Ostreococcus</em> in typical mariculture bays in northern China, laying the foundation for understanding and revealing the ecological functions of pico-phytoplankton.</div></div>","PeriodicalId":18204,"journal":{"name":"Marine environmental research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-24DOI: 10.1016/j.marenvres.2024.106766
The proliferation of pest and invasive marine macroalgae threatens coastal ecosystems, with biotic interactions, including direct effects such as grazing and indirect effects such as the trophic cascades, where one species indirectly affects another through its interactions with a third species, play a critical role in determining the resistance of local communities to these invasions. This study examines the foraging behaviour and preference of native fish communities toward native (Halopteris scoparia, Sargassum vulgare) and non-indigenous (Asparagopsis taxiformis) macroalgae using the Remote Video Foraging System (RVFS). Fifty-four weedpops were deployed across three locations to present these macroalgae, while associated epifaunal assemblages were also collected. Video analysis revealed that four common fish species displayed preference towards native macroalgae, possibly due to by the presence of zoobenthos rather than herbivory. This observation suggests that these fish species identified the macroalgae as a habitat that harboured their preferred food items. In contrast, A. taxiformis was consistently avoided, suggesting limited integration into the local food web. Site-specific variations in fish-macroalgae interactions and epifaunal diversity highlighted the complexity of these dynamics. This study contributes to understanding of the ecological implications of invasive macroalgae and supports the use of RVFS as a tool for assessing local biotic resistance against non-indigenous species in coastal ecosystems globally.
{"title":"Evaluating fish foraging behaviour on non-indigenous Asparagopsis taxiformis using a remote video foraging system","authors":"","doi":"10.1016/j.marenvres.2024.106766","DOIUrl":"10.1016/j.marenvres.2024.106766","url":null,"abstract":"<div><div>The proliferation of pest and invasive marine macroalgae threatens coastal ecosystems, with biotic interactions, including direct effects such as grazing and indirect effects such as the trophic cascades, where one species indirectly affects another through its interactions with a third species, play a critical role in determining the resistance of local communities to these invasions. This study examines the foraging behaviour and preference of native fish communities toward native (<em>Halopteris scoparia</em>, <em>Sargassum vulgare</em>) and non-indigenous (<em>Asparagopsis taxiformis</em>) macroalgae using the Remote Video Foraging System (RVFS). Fifty-four weedpops were deployed across three locations to present these macroalgae, while associated epifaunal assemblages were also collected. Video analysis revealed that four common fish species displayed preference towards native macroalgae, possibly due to by the presence of zoobenthos rather than herbivory. This observation suggests that these fish species identified the macroalgae as a habitat that harboured their preferred food items. In contrast, <em>A. taxiformis</em> was consistently avoided, suggesting limited integration into the local food web. Site-specific variations in fish-macroalgae interactions and epifaunal diversity highlighted the complexity of these dynamics. This study contributes to understanding of the ecological implications of invasive macroalgae and supports the use of RVFS as a tool for assessing local biotic resistance against non-indigenous species in coastal ecosystems globally.</div></div>","PeriodicalId":18204,"journal":{"name":"Marine environmental research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142359189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-24DOI: 10.1016/j.marenvres.2024.106770
The Northeast Pacific Ocean (NEP) is one of the important carbon sinks in the global ocean. The causes of carbon flux changes in this region have been widely studied, but the physical processes associated with large scale climate variability remain controversial primarily due to scarcity of spatially and temporally continuous observations. In this study, we constructed a high-resolution sea surface partial pressure of CO2 (pCO2) from satellite observations for the NEP from 2003 to 2020 using the machine learning based XGBoost model. By analyzing the interannual large-scale high-latitude atmospheric dynamics and ocean physical conditions over the NEP, we find that the CO2 flux density (FCO2) anomalies have a strong correlation with the Alaskan atmospheric blocking events. In the region north of 48°N, anomalous cyclones triggered by atmospheric blocking increased sea surface height (SSH), which reduced the replenishment of dissolved inorganic carbon (DIC) from deep seawater, leading to enhanced carbon uptake. By contrast, in the region south of 48°N, the increase in sea surface temperature (SST) triggered by atmospheric blocking reduced the solubility of CO2 in seawater, resulting in a decrease in regional carbon flux. These results provide new perspectives for better understanding and predicting the effects of high-latitude atmospheric dynamics on regional ocean carbon fluxes.
{"title":"Impact of Alaska atmospheric blocking on the carbon flux in the Northeast Pacific Ocean","authors":"","doi":"10.1016/j.marenvres.2024.106770","DOIUrl":"10.1016/j.marenvres.2024.106770","url":null,"abstract":"<div><div>The Northeast Pacific Ocean (NEP) is one of the important carbon sinks in the global ocean. The causes of carbon flux changes in this region have been widely studied, but the physical processes associated with large scale climate variability remain controversial primarily due to scarcity of spatially and temporally continuous observations. In this study, we constructed a high-resolution sea surface partial pressure of CO<sub>2</sub> (<em>p</em>CO<sub>2</sub>) from satellite observations for the NEP from 2003 to 2020 using the machine learning based XGBoost model. By analyzing the interannual large-scale high-latitude atmospheric dynamics and ocean physical conditions over the NEP, we find that the CO<sub>2</sub> flux density (FCO<sub>2</sub>) anomalies have a strong correlation with the Alaskan atmospheric blocking events. In the region north of 48°N, anomalous cyclones triggered by atmospheric blocking increased sea surface height (SSH), which reduced the replenishment of dissolved inorganic carbon (DIC) from deep seawater, leading to enhanced carbon uptake. By contrast, in the region south of 48°N, the increase in sea surface temperature (SST) triggered by atmospheric blocking reduced the solubility of CO<sub>2</sub> in seawater, resulting in a decrease in regional carbon flux. These results provide new perspectives for better understanding and predicting the effects of high-latitude atmospheric dynamics on regional ocean carbon fluxes.</div></div>","PeriodicalId":18204,"journal":{"name":"Marine environmental research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}