A dose-dependent bimodal switch by homologous Aux/IAA transcriptional repressors.

IF 17.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Plant Pub Date : 2024-09-02 Epub Date: 2024-08-06 DOI:10.1016/j.molp.2024.07.014
Hyung-Taeg Cho, Minsu Lee, Hee-Seung Choi, Kwang-Ho Maeng, Kyeonghoon Lee, Ha-Yeon Lee, Anindya Ganguly, Hoonyoung Park, Chang-Hoi Ho
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Abstract

Combinatorial interactions between different regulators diversify and enrich the chance of transcriptional regulation in eukaryotic cells. However, a dose-dependent functional switch of homologous transcriptional repressors has rarely been reported. Here, we show that SHY2, an auxin/indole-3-acetic acid (Aux/IAA) repressor, exhibits a dose-dependent bimodal role in auxin-sensitive root-hair growth and gene transcription in Arabidopsis, whereas other Aux/IAA homologs consistently repress the auxin responses. The co-repressor (TOPLESS [TPL])-binding affinity of a bimodal Aux/IAA was lower than that of a consistently repressing Aux/IAA. The switch of a single amino acid residue in the TPL-binding motif between the bimodal form and the consistently repressing form switched their TPL-binding affinity and transcriptional and biological roles in auxin responses. Based on these data, we propose a model whereby competition between homologous repressors with different co-repressor-binding affinities could generate a bimodal output at the transcriptional and developmental levels.

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同源 Aux/IAA 转录抑制因子的剂量依赖性双模切换。
不同调控因子之间的组合相互作用使真核细胞中转录调控的机会变得多样化和丰富。然而,同源转录抑制因子的剂量依赖性功能转换却鲜有报道。在这里,我们发现拟南芥中的叶绿素/吲哚-3-乙酸(Aux/IAA)抑制因子 SHY2 在对叶绿素敏感的根毛生长和基因转录中表现出剂量依赖性的双峰作用,而其他 Aux/IAA 同源物则始终抑制叶绿素反应。双模 Aux/IAA 的核心抑制因子(TOPLESS [TPL])结合亲和力低于持续抑制的 Aux/IAA。双峰型和持续抑制型之间 TPL 结合基序中一个氨基酸残基的改变,改变了它们的 TPL 结合亲和力以及在植物生长素反应中的转录和生物学作用。基于这些数据,我们提出了一个模型,即具有不同核心抑制因子结合亲和力的同源抑制因子之间的竞争可在转录和发育水平上产生双模输出。
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来源期刊
Molecular Plant
Molecular Plant 植物科学-生化与分子生物学
CiteScore
37.60
自引率
2.20%
发文量
1784
审稿时长
1 months
期刊介绍: Molecular Plant is dedicated to serving the plant science community by publishing novel and exciting findings with high significance in plant biology. The journal focuses broadly on cellular biology, physiology, biochemistry, molecular biology, genetics, development, plant-microbe interaction, genomics, bioinformatics, and molecular evolution. Molecular Plant publishes original research articles, reviews, Correspondence, and Spotlights on the most important developments in plant biology.
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