Genetic variation in a heat shock transcription factor modulates cold tolerance in maize.

IF 17.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Plant Pub Date : 2024-09-02 Epub Date: 2024-08-07 DOI:10.1016/j.molp.2024.07.015
Lei Gao, Lingling Pan, Yiting Shi, Rong Zeng, Minze Li, Zhuoyang Li, Xuan Zhang, Xiaoming Zhao, Xinru Gong, Wei Huang, Xiaohong Yang, Jinsheng Lai, Jianru Zuo, Zhizhong Gong, Xiqing Wang, Weiwei Jin, Zhaobin Dong, Shuhua Yang
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Abstract

Understanding how maize (Zea mays) responds to cold stress is crucial for facilitating breeding programs of cold-tolerant varieties. Despite extensive utilization of the genome-wide association study (GWAS) approach for exploring favorable natural alleles associated with maize cold tolerance, few studies have successfully identified candidate genes that contribute to maize cold tolerance. In this study, we used a diverse panel of inbred maize lines collected from different germplasm sources to perform a GWAS on variations in the relative injured area of maize true leaves during cold stress-a trait very closely correlated with maize cold tolerance. We identified HSF21, which encodes a B-class heat shock transcription factor (HSF) that positively regulates cold tolerance at both the seedling and germination stages. Natural variations in the promoter of the cold-tolerant HSF21Hap1 allele led to increased HSF21 expression under cold stress by inhibiting binding of the basic leucine zipper bZIP68 transcription factor, a negative regulator of cold tolerance. By integrating transcriptome deep sequencing, DNA affinity purification sequencing, and targeted lipidomic analysis, we revealed the function of HSF21 in regulating lipid metabolism homeostasis to modulate cold tolerance in maize. In addition, we found that HSF21 confers maize cold tolerance without incurring yield penalties. Collectively, this study establishes HSF21 as a key regulator that enhances cold tolerance in maize, providing valuable genetic resources for breeding of cold-tolerant maize varieties.

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热休克转录因子的遗传变异调节玉米的耐寒性。
了解玉米(Zea mays L.)如何应对冷胁迫对于促进耐寒品种的育种计划至关重要。尽管全基因组关联研究(GWAS)方法被广泛用于探索与玉米耐寒性相关的有利天然等位基因,但成功鉴定出有助于玉米耐寒性的候选基因的报道却很少。在本研究中,我们利用从不同种质资源中收集的各种玉米近交系,对玉米真叶在冷胁迫时相对受伤面积的变化(这是与玉米耐寒性最密切相关的性状)进行了基因关联分析,并鉴定出编码B类热休克转录因子的HSF21,它在幼苗和发芽阶段都对耐寒性有正向调控作用。耐寒的 HSF21Hap1 等位基因启动子中的自然变异通过抑制耐寒的负调控因子 bZIP68 转录因子的结合,导致 HSF21 在冷胁迫下的表达增加。通过整合转录组深度测序、DNA亲和纯化测序和靶向脂质体分析,我们揭示了HSF21在调节脂质代谢平衡以调节玉米耐寒性方面的功能。此外,HSF21 在赋予玉米耐寒性的同时不会造成产量损失。这项研究由此确定了 HSF21 是增强玉米耐寒性的关键调控因子,从而为培育耐寒玉米品种提供了宝贵的遗传资源。
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来源期刊
Molecular Plant
Molecular Plant 植物科学-生化与分子生物学
CiteScore
37.60
自引率
2.20%
发文量
1784
审稿时长
1 months
期刊介绍: Molecular Plant is dedicated to serving the plant science community by publishing novel and exciting findings with high significance in plant biology. The journal focuses broadly on cellular biology, physiology, biochemistry, molecular biology, genetics, development, plant-microbe interaction, genomics, bioinformatics, and molecular evolution. Molecular Plant publishes original research articles, reviews, Correspondence, and Spotlights on the most important developments in plant biology.
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