Tissue scaffolds mimicking hierarchical bone morphology as biomaterials for oral maxillofacial surgery with augmentation: structure, properties, and performance evaluation forin vitrotesting.
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引用次数: 0
Abstract
In this study, tissue scaffolds mimicking hierarchical morphology are constructed and proposed for bone augmentation. The scaffolds are fabricated using lyophilization, before coating them with collagen (Col). Subsequently, the Col-coated scaffolds undergo a second lyophilization, followed by silk fibroin (SF) coating, and a third lyophilization. Thereafter, the scaffolds are divided into six groups with varying ratios of Col to SF: Col/SF = 7:3, 5:5, 3:7, 10:0, and 0:10, with an SF scaffold serving as the control group. The scaffold morphology is examined using a scanning electron microscope, while molecular and structural formations are characterized by Fourier transform infrared spectrometer and differential scanning calorimeter, respectively. Physical and mechanical properties including swelling and compression are tested. Biological functions are assessed throughin vitroosteoblast cell culturing. Biomarkers indicative of bone formation-cell viability and proliferation, alkaline phosphatase activity, and calcium content-are analyzed. Results demonstrate that scaffolds coated with Col and SF exhibit sub-porous formations within the main pore. The molecular formation reveals interactions between the hydrophilic groups of Col and SF. The scaffold structure contains bound water and SF formation gets disrupted by Col. Physical and mechanical properties are influenced by the Col/SF ratio and morphology due to coating. The biological functions of scaffolds with Col and SF coating show enhanced potential for promoting bone tissue formation, particularly the Col/SF (7:3) ratio, which is most suitable for bone augmentation in small defect areas.
本研究构建了模仿分层形态的组织支架,并建议用于骨增量。先用冻干法制作支架,然后在支架上涂覆胶原蛋白(Col)。随后,涂有胶原蛋白的支架进行第二次冻干,然后涂上丝纤维素(SF),再进行第三次冻干。之后,支架被分为六组,Col 与 SF 的比例各不相同:Col/SF = 7:3、5:5、3:7、10:0 和 0:10,SF 支架作为对照组。使用扫描电子显微镜观察支架的形态,并分别使用傅立叶变换红外光谱仪和差示扫描量热仪对分子和结构形态进行表征。此外,还测试了包括膨胀和压缩在内的物理和机械性能。生物功能通过体外成骨细胞培养进行评估。分析了表明骨形成的生物标志物--细胞活力和增殖、碱性磷酸酶活性和钙含量。结果表明,涂有 Col 和 SF 的支架在主孔内呈现出次孔形态。分子的形成揭示了 Col 和 SF 的亲水基团之间的相互作用。支架结构中含有结合水,而 SF 的形成会受到胶原蛋白的干扰。物理和机械性能受 Col/SF 比例和涂层形态的影响。带有 Col 和 SF 涂层的支架的生物功能显示出促进骨组织形成的更大潜力,尤其是 Col/SF (7:3)比例,最适合用于小缺损区域的骨增量。