Adsorption of extracellular lipase in a packed-bed reactor: an alternative immobilization approach.

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Bioprocess and Biosystems Engineering Pub Date : 2024-10-01 Epub Date: 2024-08-05 DOI:10.1007/s00449-024-03066-5
Amanda Noli Freitas, Daniela Remonatto, Rodney Helder Miotti Junior, João Francisco Cabral do Nascimento, Adriana Candido da Silva Moura, Valéria de Carvalho Santos Ebinuma, Ariela Veloso de Paula
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Abstract

In light of the growing demand for novel biocatalysts and enzyme production methods, this study aimed to evaluate the potential of Aspergillus tubingensis for producing lipase under submerged culture investigating the influence of culture time and inducer treatment. Moreover, this study also investigated conditions for the immobilization of A. tubingensis lipase by physical adsorption on styrene-divinylbenzene beads (Diaion HP-20), for these conditions to be applied to an alternative immobilization system with a packed-bed reactor. Furthermore, A. tubingensis lipase and its immobilized derivative were characterized in terms of their optimal ranges of pH and temperature. A. tubingensis was shown to be a good producer of lipase, obviating the need for inducer addition. The enzyme extract had a hydrolytic activity of 23 U mL-1 and achieved better performance in the pH range of 7.5 to 9.0 and in the temperature range of 20 to 50 °C. The proposed immobilization system was effective, yielding an immobilized derivative with enhanced hydrolytic activity (35 U g-1), optimum activity over a broader pH range (5.6 to 8.4), and increased tolerance to high temperatures (40 to 60 ℃). This research represents a first step toward lipase production from A. tubingensis under a submerged culture and the development of an alternative immobilization system with a packed-bed reactor. The proposed system holds promise for saving time and resources in future industrial applications.

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细胞外脂肪酶在填料床反应器中的吸附:另一种固定化方法。
鉴于对新型生物催化剂和酶生产方法的需求日益增长,本研究旨在评估管曲霉在浸没培养条件下生产脂肪酶的潜力,调查培养时间和诱导剂处理的影响。此外,本研究还调查了管曲霉脂肪酶在苯乙烯-二乙烯基苯珠(Diaion HP-20)上的物理吸附固定条件,以便将这些条件应用于填料床反应器的替代固定系统。此外,还对管氏脂肪酶及其固定化衍生物的最佳 pH 值和温度范围进行了表征。结果表明,管状芽孢杆菌是一种很好的脂肪酶生产者,无需添加诱导剂。该酶提取物的水解活性为 23 U mL-1,在 pH 值为 7.5 至 9.0 和温度为 20 至 50 ℃ 的范围内性能更佳。拟议的固定化系统是有效的,产生的固定化衍生物具有更高的水解活性(35 U g-1),在更宽的 pH 值范围(5.6 至 8.4)内具有最佳活性,对高温(40 至 60 ℃)的耐受性更强。这项研究标志着管氏酵母在浸没培养条件下生产脂肪酶迈出了第一步,并开发出了一种使用填料床反应器的替代固定化系统。拟议的系统有望在未来的工业应用中节省时间和资源。
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来源期刊
Bioprocess and Biosystems Engineering
Bioprocess and Biosystems Engineering 工程技术-工程:化工
CiteScore
7.90
自引率
2.60%
发文量
147
审稿时长
2.6 months
期刊介绍: Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.
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