Evaluation of an integrated activated partial thromboplastin time (Cephen LS/Cephen) for the detection of lupus anticoagulant

IF 2.2 4区 医学 Q3 HEMATOLOGY International Journal of Laboratory Hematology Pub Date : 2024-08-05 DOI:10.1111/ijlh.14349
Adrien Thiriet, Vincent Poindron, Laurent Sattler, Jordan Wimmer, Delphine Rolland, Anne-Sophie Korganow, Laurent Mauvieux, Agathe Herb
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Abstract

Introduction

It is recommended to use two chronometric assays of different principles for the diagnosis of lupus anticoagulant (LA), consisting in diluted Russell Viper Venom Time (dRVVT) and activated Partial Thromboplastin Time (aPTT). Yet, there are only a few integrated aPTT assays; this study aims to evaluate one of them: Cephen LS/Cephen (Hyphen Biomed).

Method

249 samples of patients were included in this study. Normal reference ranges were determined with platelet-poor plasma (PPP) from healthy blood donors. Performances were then evaluated by comparing Cephen LS/Cephen test results to the results of the laboratory's reference assay for the diagnosis of LA and to clinical data, both on non-anticoagulated and anticoagulated patients' samples (Unfractioned heparin (UFH), Low Molecular Weight Heparin (LMWH), Vitamin K Antagonists (VKA) and apixaban). Interference of UFH, LMWH and VKA were also evaluated thanks to spiking experiment of increasing heparin concentrations or factor deficiency.

Results

Cephen LS/Cephen test had 48.6% sensitivity towards LA. Although UFH and VKA seemed to interfere with this assay and were likely to cause false negative, LMWH and apixaban did not.

Finally, combination of Cephen LS/ Cephen with dRVVT had 89.0% sensitivity.

Conclusion

Cephen LS/Cephen seems relevant for LA diagnosis, in combination with dRVVT, and might be used in patients undergoing LMWH or apixaban therapy.

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评估用于检测狼疮抗凝物的综合活化部分凝血活酶时间(Cephen LS/Cephen)。
导言:在诊断狼疮抗凝物(LA)时,建议使用两种原理不同的计时测定法,即稀释罗素蝰蛇毒时间(dRVVT)和活化部分凝血活酶时间(aPTT)。然而,目前只有少数几种整合式 aPTT 检测方法;本研究旨在对其中一种方法进行评估:方法:本研究纳入了 249 份患者样本。用健康献血者的贫血小板血浆(PPP)确定正常参考范围。然后将 Cephen LS/Cephen 检测结果与实验室诊断 LA 的参考检测结果以及临床数据(非抗凝和抗凝患者样本(非减量肝素(UFH)、低分子量肝素(LMWH)、维生素 K 拮抗剂(VKA)和阿哌沙班))进行比较,评估其性能。通过增加肝素浓度或因子缺乏的加标实验,还评估了 UFH、LMWH 和 VKA 的干扰:结果:Cephen LS/Cephen 试验对 LA 的灵敏度为 48.6%。尽管 UFH 和 VKA 似乎会干扰该检测,并可能导致假阴性,但 LMWH 和阿哌沙班不会。最后,Cephen LS/ Cephen 与 dRVVT 的组合具有 89.0% 的灵敏度:结论:Cephen LS/Cephen 与 dRVVT 联用似乎与 LA 诊断相关,可用于接受 LMWH 或阿哌沙班治疗的患者。
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来源期刊
CiteScore
4.50
自引率
6.70%
发文量
211
审稿时长
6-12 weeks
期刊介绍: The International Journal of Laboratory Hematology provides a forum for the communication of new developments, research topics and the practice of laboratory haematology. The journal publishes invited reviews, full length original articles, and correspondence. The International Journal of Laboratory Hematology is the official journal of the International Society for Laboratory Hematology, which addresses the following sub-disciplines: cellular analysis, flow cytometry, haemostasis and thrombosis, molecular diagnostics, haematology informatics, haemoglobinopathies, point of care testing, standards and guidelines. The journal was launched in 2006 as the successor to Clinical and Laboratory Hematology, which was first published in 1979. An active and positive editorial policy ensures that work of a high scientific standard is reported, in order to bridge the gap between practical and academic aspects of laboratory haematology.
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