The effect of light availability and light wavelength on growth, 2-MIB biosynthesis, and 2-MIB-related gene expression in Pseudanabaena foetida var. intermedia

IF 2.6 3区生物学 Q3 MICROBIOLOGY Archives of Microbiology Pub Date : 2024-08-06 DOI:10.1007/s00203-024-04099-w

Kaushalya Dayarathne, Toshiki Ishikawa, Aikeranmu Kadeer, Masatoshi Yamaguchi, Maki Kawai-Yamada

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Abstract

2-methylisoborneol (2-MIB) is an odiferous metabolite mainly produced by cyanobacteria, contributing to taste and odor problems in drinking water. The mechanisms involved in 2-MIB biosynthesis in cyanobacteria are not yet completely understood. This study investigated the effect of light availability and wavelength on growth, 2-MIB synthesis, and related gene expression in Pseudanabaena foetida var. intermedia. A significantly lower 2-MIB production was observed in P. foetida var. intermedia during the dark period of a 12-h photoperiod. Exposure to green light resulted in a significant decrease in 2-MIB production compared to white light and red light. The relative expression levels of 2-MIB-related genes in P. foetida var. intermedia were significantly lower during the dark period of a 12-h photoperiod and when cultured under green light. The expression of 2-MIB-related genes in cyanobacteria appears to be light-dependent. This study suggests that the demand for photopigment synthesis under unfavorable light conditions affects the 2-MIB synthesis in cyanobacteria.

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光照条件和光波长对福氏拟尾柱虫变种生长、2-MIB 生物合成和 2-MIB 相关基因表达的影响

2- 甲基异龙脑（2-MIB）是一种有异味的代谢物，主要由蓝藻产生，造成饮用水的味道和气味问题。蓝藻中 2-MIB 的生物合成机制尚不完全清楚。本研究调查了光照和波长对 Pseudanabaena foetida var.在 12 小时光周期的黑暗期，P. foetida var. intermedia 的 2-MIB 产量明显较低。与白光和红光相比，绿光导致 2-MIB 生成量明显减少。在 12 小时光周期的黑暗期和绿光下培养时，P. foetida var. intermedia 中 2-MIB 相关基因的相对表达水平明显较低。蓝藻中 2-MIB 相关基因的表达似乎与光照有关。这项研究表明，在不利的光照条件下，对光色素合成的需求会影响蓝藻中 2-MIB 的合成。

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来源期刊

Archives of Microbiology 生物-微生物学

CiteScore

4.90

自引率

3.60%

发文量

601

审稿时长

3 months

期刊介绍： Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.