The effect of light availability and light wavelength on growth, 2-MIB biosynthesis, and 2-MIB-related gene expression in Pseudanabaena foetida var. intermedia

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-08-06 DOI:10.1007/s00203-024-04099-w

Kaushalya Dayarathne, Toshiki Ishikawa, Aikeranmu Kadeer, Masatoshi Yamaguchi, Maki Kawai-Yamada

{"title":"The effect of light availability and light wavelength on growth, 2-MIB biosynthesis, and 2-MIB-related gene expression in Pseudanabaena foetida var. intermedia","authors":"Kaushalya Dayarathne, Toshiki Ishikawa, Aikeranmu Kadeer, Masatoshi Yamaguchi, Maki Kawai-Yamada","doi":"10.1007/s00203-024-04099-w","DOIUrl":null,"url":null,"abstract":"<div>2-methylisoborneol (2-MIB) is an odiferous metabolite mainly produced by cyanobacteria, contributing to taste and odor problems in drinking water. The mechanisms involved in 2-MIB biosynthesis in cyanobacteria are not yet completely understood. This study investigated the effect of light availability and wavelength on growth, 2-MIB synthesis, and related gene expression in Pseudanabaena foetida var. intermedia. A significantly lower 2-MIB production was observed in P. foetida var. intermedia during the dark period of a 12-h photoperiod. Exposure to green light resulted in a significant decrease in 2-MIB production compared to white light and red light. The relative expression levels of 2-MIB-related genes in P. foetida var. intermedia were significantly lower during the dark period of a 12-h photoperiod and when cultured under green light. The expression of 2-MIB-related genes in cyanobacteria appears to be light-dependent. This study suggests that the demand for photopigment synthesis under unfavorable light conditions affects the 2-MIB synthesis in cyanobacteria.</div>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s00203-024-04099-w","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}

引用次数: 0

Abstract

2-methylisoborneol (2-MIB) is an odiferous metabolite mainly produced by cyanobacteria, contributing to taste and odor problems in drinking water. The mechanisms involved in 2-MIB biosynthesis in cyanobacteria are not yet completely understood. This study investigated the effect of light availability and wavelength on growth, 2-MIB synthesis, and related gene expression in Pseudanabaena foetida var. intermedia. A significantly lower 2-MIB production was observed in P. foetida var. intermedia during the dark period of a 12-h photoperiod. Exposure to green light resulted in a significant decrease in 2-MIB production compared to white light and red light. The relative expression levels of 2-MIB-related genes in P. foetida var. intermedia were significantly lower during the dark period of a 12-h photoperiod and when cultured under green light. The expression of 2-MIB-related genes in cyanobacteria appears to be light-dependent. This study suggests that the demand for photopigment synthesis under unfavorable light conditions affects the 2-MIB synthesis in cyanobacteria.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

光照条件和光波长对福氏拟尾柱虫变种生长、2-MIB 生物合成和 2-MIB 相关基因表达的影响

2- 甲基异龙脑（2-MIB）是一种有异味的代谢物，主要由蓝藻产生，造成饮用水的味道和气味问题。蓝藻中 2-MIB 的生物合成机制尚不完全清楚。本研究调查了光照和波长对 Pseudanabaena foetida var.在 12 小时光周期的黑暗期，P. foetida var. intermedia 的 2-MIB 产量明显较低。与白光和红光相比，绿光导致 2-MIB 生成量明显减少。在 12 小时光周期的黑暗期和绿光下培养时，P. foetida var. intermedia 中 2-MIB 相关基因的相对表达水平明显较低。蓝藻中 2-MIB 相关基因的表达似乎与光照有关。这项研究表明，在不利的光照条件下，对光色素合成的需求会影响蓝藻中 2-MIB 的合成。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊